Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	150,828	A→C	6.1%	L41R (CTG→CGG) ‡	PP_0142 ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	150,828	0	A	C	6.1%	260.2 / 6.9	82	L41R (CTG→CGG) ‡	PP_0142	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (34/43);  new base C (3/2);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

AGCGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAGG  >  NC_002947/150681‑150976                                                                                                                                                     |                                                                                                                                                     agcGCCTTGCGGGCTTCAGGCAGCGCGTGGGTGCACGGCGTCAGGCGCTCGGAGCCTACGAGTTCGGCCAGCAGCGAAGCGCCGGCAGGTGTCAAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCa                                                                                                                                                      <  1:289180/149‑1 (MQ=255)       tggctggcTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGgcgc                                                                                                                                                 >  1:299818/1‑147 (MQ=255)       tggctggcTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGgcgc                                                                                                                                                 <  2:299818/147‑1 (MQ=255)           tggctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCCTGcgctcgcat                                                                                                                                           <  2:373122/149‑3 (MQ=255)             gctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCCTGcgctcgcatt                                                                                                                                          <  1:340993/148‑4 (MQ=255)             gctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCtctc                                                                                                                                         <  5:224296/149‑1 (MQ=255)             gctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCtctc                                                                                                                                         <  6:169461/149‑1 (MQ=255)               ttCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGctct                                                                                                                                          >  5:47193/1‑146 (MQ=255)               ttCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGctct                                                                                                                                          <  6:47193/146‑1 (MQ=255)               ttCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGCGCAATGCGAGCGCAGGCGCTCGCTCTcgc                                                                                                                                       >  8:298480/1‑149 (MQ=255)                 cAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCgcgc                                                                                                                                     >  8:306188/1‑149 (MQ=255)                  aGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCgcgc                                                                                                                                     <  3:239183/148‑1 (MQ=255)                  aGGCAGCGCGTCGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCt                                                                                                                                    >  7:8109/1‑149 (MQ=255)                     cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGa                                                                                                                                 <  5:183254/149‑1 (MQ=255)                     cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGa                                                                                                                                 <  6:341599/149‑1 (MQ=255)                     cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGa                                                                                                                                 <  8:278756/149‑1 (MQ=255)                            tGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCATGCGCTCGCTCTCGCTCTTGAGGTTgtc                                                                                                                          <  6:171100/149‑3 (MQ=255)                            tGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGc                                                                                                                          <  2:156765/149‑1 (MQ=255)                            tGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGc                                                                                                                          <  7:74407/149‑1 (MQ=255)                               gTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCg                                                                                                                         >  7:117188/1‑147 (MQ=255)                                tGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAg                                                                                                                      >  6:342780/1‑149 (MQ=255)                                tGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGGGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAg                                                                                                                      >  5:80733/1‑149 (MQ=255)                                    gTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGtgtg                                                                                                                  >  8:185941/1‑149 (MQ=255)                                    gTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGtgtg                                                                                                                  >  6:232205/1‑149 (MQ=255)                                     tGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGc                                                                                                                 >  6:112838/1‑149 (MQ=255)                                     tGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGc                                                                                                                 <  4:67828/149‑1 (MQ=255)                                        gACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAg                                                                                                              >  2:68345/1‑149 (MQ=255)                                        gACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTAGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAg                                                                                                              >  6:123293/1‑149 (MQ=255)                                             gcgcTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTc                                                                                                          >  2:129123/1‑148 (MQ=255)                                               gcTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAg                                                                                                       <  1:175918/149‑1 (MQ=255)                                                   ggtgCCTAGGAGTTCGGCCAGGAGCGATGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTTGCTTAGGTCGGCGACGGTGTCGGCAGTGCAATGCTAGCGCAGTCGCTCGCTCTCGCGCTTGATGTTGGCGTAGTGTGGCAGGGTCCAGTCAc                                                                                                   <  1:182347/146‑1 (MQ=255)                                                   ggAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAc                                                                                                   <  8:117188/149‑1 (MQ=255)                                                   ggAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAc                                                                                                   <  1:190260/149‑1 (MQ=255)                                                     aGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCg                                                                                                 <  3:57797/149‑1 (MQ=255)                                                       ccTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCgg                                                                                                >  5:13661/1‑148 (MQ=255)                                                       ccTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTAGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCgg                                                                                                >  8:187646/1‑148 (MQ=255)                                                         tAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTaa                                                                                             >  4:348965/1‑149 (MQ=255)                                                                 cGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcag                                                                                      <  3:61742/148‑1 (MQ=255)                                                                 cGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcag                                                                                      <  2:165869/148‑1 (MQ=255)                                                                  ggCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcaggc                                                                                    <  7:187646/149‑1 (MQ=255)                                                                     cagcagCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGcaggcagg                                                                                 >  7:210241/1‑149 (MQ=255)                                                                     cagcagAGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGTCTGAGGTCGGCGAAGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCTCTCTAGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTACATTCACCGGTAATACGcaggcagg                                                                                 >  6:102531/1‑149 (MQ=255)                                                                      agcagcGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                <  8:8109/149‑1 (MQ=255)                                                                      agcagcGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                >  5:134632/1‑149 (MQ=255)                                                                       gcagcGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGcc                                                                               <  1:68345/149‑1 (MQ=255)                                                                           cGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGtt                                                                           >  3:179022/1‑149 (MQ=255)                                                                                  ccGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgctgct                                                                    >  5:151067/1‑149 (MQ=255)                                                                                   cGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGtt                                                                           >  6:104858/1‑141 (MQ=255)                                                                                   cGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGtt                                                                           <  5:104858/141‑1 (MQ=255)                                                                                   cGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGctgctg                                                                   <  4:212605/149‑1 (MQ=255)                                                                                      cA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGgtg                                                                >  2:105877/1‑149 (MQ=255)                                                                                       a‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                >  7:258329/1‑132 (MQ=255)                                                                                       a‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                <  8:258329/132‑1 (MQ=255)                                                                                          tgtCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTcc                                                             <  1:24873/149‑1 (MQ=255)                                                                                            tCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAg                                                           >  3:16359/1‑149 (MQ=255)                                                                                            tCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAg                                                           >  6:271870/1‑149 (MQ=255)                                                                                              cAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAgg                                                          <  1:147791/148‑1 (MQ=255)                                                                                               aGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGt                                                        <  8:210241/149‑1 (MQ=255)                                                                                               aGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGt                                                        <  5:271870/149‑1 (MQ=255)                                                                                                  cggcCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGGCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGgc                                                     <  1:94413/149‑1 (MQ=255)                                                                                                     cccAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGCGGCGCt                                                  <  5:112838/149‑1 (MQ=255)                                                                                                       cAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTgg                                                <  6:236021/149‑1 (MQ=255)                                                                                                        aGCTGGCTGAGGTCTGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTggg                                               <  5:232205/149‑1 (MQ=255)                                                                                                             gctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          <  3:348965/149‑1 (MQ=255)                                                                                                             gctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          <  4:179022/149‑1 (MQ=255)                                                                                                                 aGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTc                                        <  6:237435/147‑1 (MQ=255)                                                                                                                  ggTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCa                                       >  7:45345/1‑147 (MQ=255)                                                                                                                    tCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          >  4:254548/1‑142 (MQ=255)                                                                                                                    tCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          <  3:254548/142‑1 (MQ=255)                                                                                                                       gCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTc                                >  6:315583/1‑149 (MQ=255)                                                                                                                        cGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTcc                               <  5:342780/149‑1 (MQ=255)                                                                                                                              tgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCaa                         <  6:151067/149‑1 (MQ=255)                                                                                                                                 cGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGgtgt                                                               >  1:14446/1‑108 (MQ=255)                                                                                                                                 cGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGgtgt                                                               <  2:14446/108‑1 (MQ=255)                                                                                                                                  ggCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGt                                                                            >  2:312634/1‑94 (MQ=255)                                                                                                                                     aGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTc                  >  2:284579/1‑149 (MQ=255)                                                                                                                                     aGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTc                  <  5:123293/149‑1 (MQ=255)                                                                                                                                       ggCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCgg                >  3:298013/1‑149 (MQ=255)                                                                                                                                         gagagCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGgcg              >  3:5812/5‑149 (MQ=255)                                                                                                                                           gagCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGgcgc             >  1:27984/3‑148 (MQ=255)                                                                                                                                            agCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCGAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCt            >  2:262627/2‑148 (MQ=255)                                                                                                                                              cGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAg         <  7:298480/149‑1 (MQ=255)                                                                                                                                                    cAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAg   <  4:16359/149‑1 (MQ=255)                                                                                                                                                     aGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAgg  <  8:45345/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     AGCGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCA‑GTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAGG  >  NC_002947/150681‑150976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑