Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,272,745 G→T 6.2% E147* (GAA→TAA)  PP_1113 → pyridoxal‑phosphate dependent enzyme family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,272,7450GT 6.2% 199.2 / 3.2 64E147* (GAA→TAA) PP_1113pyridoxal‑phosphate dependent enzyme family protein
Reads supporting (aligned to +/- strand):  ref base G (34/26);  new base T (2/2);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

CAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACGGTACTGGTCGATC  >  NC_002947/1272597‑1272886
                                                                                                                                                    |                                                                                                                                             
cAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCGGGATAACCACGCCGCGCTGGGCAAGGCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGAGCGCCAGTg                                                                                                                                               <  2:265715/149‑1 (MQ=255)
       aTGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCCGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCAGCGAAGCCCATCGTTATCAGACCGCCCGTGAAATGGc                                                                                                                                        >  8:201714/1‑149 (MQ=255)
          cTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGCCCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGt                                                                                                                                     >  5:168953/1‑149 (MQ=255)
             aTGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGc                                                                                                                                   <  2:288752/148‑1 (MQ=255)
                gCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAAc                                                                                                                                <  7:75465/148‑1 (MQ=255)
                  cGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACAGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGa                                                                                                                             <  2:196263/149‑1 (MQ=255)
                    gTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGAcc                                                                                                                           <  1:236084/149‑1 (MQ=255)
                       ccACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAAc                                                                                                                        >  5:353440/1‑149 (MQ=255)
                           acCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCg                                                                                                                    >  8:237861/1‑149 (MQ=255)
                             cgaaGGACGGGGTGATGCATCACCTGGATAACCAGGCCGCGCTTGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCggg                                                                                                                  <  8:211289/147‑1 (MQ=255)
                             ccAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTTCACTGGCGGt                                                                                                                                     >  5:336127/1‑130 (MQ=255)
                             ccAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTTCACTGGCGGt                                                                                                                                     <  6:336127/130‑1 (MQ=255)
                             ccAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCggg                                                                                                                  <  3:97710/149‑1 (MQ=255)
                                   aCGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAggg                                                                                                            <  8:162169/149‑1 (MQ=255)
                                      tggtgATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGggcgg                                                                                                         <  2:119860/149‑1 (MQ=255)
                                          gATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGggcggc                                                                                                        >  5:23578/1‑146 (MQ=255)
                                          gATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGggcggc                                                                                                        <  6:23578/146‑1 (MQ=255)
                                            tGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATc                                                                                                   <  6:353440/149‑1 (MQ=255)
                                             gCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATcc                                                                                                  >  7:191225/1‑149 (MQ=255)
                                                  aCCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTc                                                                                             >  3:300733/1‑149 (MQ=255)
                                                  aCCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTc                                                                                             >  4:152949/1‑149 (MQ=255)
                                                             cAGCCCGCGCCGCGCAAGCCCCACTTCTCCCCCGCCCCGCGCACCACCCCGGTGGGCATCGACGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGc                                                                                  <  4:300733/149‑1 (MQ=255)
                                                               ggCCGCTCTGGGCAAGTCATACTTCTAGACCGACCAGTTCAACAACCCGGGTGGCATCGAAGCCCATCTTTATCAGACCGCCAGTGAACTGGCCGTTCAACTGACCAACGCCGGTGCAGGGCGGCAGATCCTTTTCGTGGGCTGTGCgg                                                                                <  6:168953/149‑1 (MQ=255)
                                                                    cgcTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACt                                                                           >  1:218039/1‑149 (MQ=255)
                                                                               tCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGttt                                                                >  3:334213/1‑149 (MQ=255)
                                                                                 aTACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCTGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCa                                                              >  5:322474/1‑149 (MQ=255)
                                                                                    cTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACgg                                                           <  2:218039/149‑1 (MQ=255)
                                                                                       cTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCtg                                                                                     <  4:65993/120‑1 (MQ=255)
                                                                                       cTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCtg                                                                                     >  3:65993/1‑120 (MQ=255)
                                                                                         acacCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTAt                                                       >  6:320789/1‑148 (MQ=255)
                                                                                          cacCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGCTCCTGTTCCTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCa                                                     >  5:127957/1‑149 (MQ=255)
                                                                                          cacCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCa                                                     >  4:244592/1‑149 (MQ=255)
                                                                                             cGACCAGATCAACAACCCGGTGGGCATCGAAGCCCAACGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGCGGCAGGGCGGCACATCCTGTACGTGCGCTGTGCGGGAACTGGCGCCAGTTTCACGGGTATCAccc                                                  >  8:184068/1‑149 (MQ=255)
                                                                                                ccAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTgg                                               <  6:153654/149‑1 (MQ=255)
                                                                                                   gTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATc                                                      >  4:16238/1‑139 (MQ=255)
                                                                                                   gTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATc                                                      <  3:16238/139‑1 (MQ=255)
                                                                                                    ttCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCt                                           >  7:173276/1‑149 (MQ=255)
                                                                                                       aacaacCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCt                                           >  7:308796/1‑146 (MQ=255)
                                                                                                       aacaacCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCt                                           <  8:308796/146‑1 (MQ=255)
                                                                                                        acaacCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAg                                       <  3:152949/149‑1 (MQ=255)
                                                                                                        acaacCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAg                                       >  6:31274/1‑149 (MQ=255)
                                                                                                        acaacCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGACAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTTGGGCTGAAg                                       >  7:94144/1‑149 (MQ=255)
                                                                                                         caacCCGGTGGGCATCGATGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTgggg                                             <  8:77092/142‑1 (MQ=255)
                                                                                                         caacCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGAGAACTCGCCGTGCAACTGACCAACGCAGGGGCAGGGCCGCAGATACTGTTCGTGGGCTGTGCGGGTACTGCAGCCAGATTCACGGGTATCACCCTGGGGcacatcg                                      >  7:77092/1‑143 (MQ=255)
                                                                                                           taacgatGGGCTTCGATGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGAc                                    >  3:266915/7‑149 (MQ=255)
                                                                                                              cgatGGGCTTCGATGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAg                                       <  1:270745/140‑1 (MQ=255)
                                                                                                              cgatGGGCTTCGATGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAg                                       >  2:270745/4‑143 (MQ=255)
                                                                                                              cGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCAt                                 <  4:334213/149‑1 (MQ=255)
                                                                                                               ggTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATg                                >  6:305649/1‑149 (MQ=255)
                                                                                                                    gCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTc                           >  5:73796/1‑149 (MQ=255)
                                                                                                                           aGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACgtgt                    >  3:62023/1‑149 (MQ=255)
                                                                                                                               cATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACgg                                                           <  8:271859/106‑1 (MQ=255)
                                                                                                                               cATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACgg                                                           >  7:271859/1‑106 (MQ=255)
                                                                                                                               cATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGAc                >  6:163177/1‑149 (MQ=255)
                                                                                                                                aTCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACg               >  5:181901/1‑149 (MQ=255)
                                                                                                                                    ttATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACGGTAc           <  6:322474/149‑1 (MQ=255)
                                                                                                                                    ttATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACGGTAc           >  1:88690/1‑149 (MQ=255)
                                                                                                                                     tATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGgc                                                                        >  1:148150/1‑87 (MQ=255)
                                                                                                                                     tATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGgc                                                                        <  2:148150/87‑1 (MQ=255)
                                                                                                                                         aGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACGGTACTGGt       <  6:181901/148‑1 (MQ=255)
                                                                                                                                          cacggCCAGTTCACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACGGTACTGGTc      >  5:8727/5‑148 (MQ=255)
                                                                                                                                           acggCCAGTTCACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACGGTACTGGTCGa    <  6:8727/146‑1 (MQ=255)
                                                                                                                                           aCCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACgg              >  2:182071/1‑139 (MQ=255)
                                                                                                                                           aCCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACgg              <  1:182071/139‑1 (MQ=255)
                                                                                                                                             cGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTAGTGGGCTGTGCGGGTACTGGAGCCAGTTTCACGGGTATCAACCTGGGGCTGAAGGACCATGCATTCGACGTGTCGACGGTACTGGTCGATc  >  5:206862/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAACCCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATCCTGTTCGTGGGCTGTGCGGGTACTGGCGCCAGTTTCACGGGTATCACCCTGGGGCTGAAGGACCATGGCTTCGACGTGTCGACGGTACTGGTCGATC  >  NC_002947/1272597‑1272886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑