Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,285,037 T→C 7.5% L90L (TTG→CTG) ‡ PP_1122 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,285,0370TC 7.5% 225.9 / 5.6 80L90L (TTG→CTG) ‡PP_1122OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (32/40);  new base C (3/3);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCGG  >  NC_002947/1284889‑1285184
                                                                                                                                                     |                                                                                                                                                   
cAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTAGCCCATCTGCAGCCTGGTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑t                                                                                                                                                     >  1:111056/1‑149 (MQ=255)
  gCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTg                                                                                                                                                   >  4:34348/1‑149 (MQ=255)
       cTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGGTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGgc                                                                                                                                                <  3:291449/147‑1 (MQ=255)
        tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGgcgc                                                                                                                                              >  7:228542/1‑148 (MQ=255)
          aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAt                                                                                                                                           >  7:210102/1‑149 (MQ=255)
          aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAt                                                                                                                                           <  3:224222/149‑1 (MQ=255)
           cGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAtt                                                                                                                                          <  3:186344/149‑1 (MQ=255)
            gggCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCGTTGGTGCCATCGAGAGTTCTTCATGGGCCGCCGGTGGTGGCGCC‑GTGGGCGCCATTg                                                                                                                                         >  8:135020/1‑149 (MQ=255)
             ggCTGCGCTACGCACAGCTATGGCTGCGCGCCCCTCAATCAAAGGCCCTGGCCCTTCTGCCGCCTTCTGGGGGGATTGGTGGGTGTTGGCCTTGTTGCAATGGATATTTCTTCCTGGGCCGCCTGTGGTGGCGCC‑CAAGGCGCCaccac                                                                                                                                        <  4:185316/149‑5 (MQ=255)
                tGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGCCCGCCGGCAATGGCGCC‑CAAGGCGCCaccaccgg                                                                                                                                     >  1:130655/1‑142 (MQ=38)
                   gcTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCcggcgg                                                                                                                                  <  8:210102/149‑1 (MQ=255)
                    cTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGcc                                                                                                                                             >  5:368326/1‑137 (MQ=255)
                    cTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGcc                                                                                                                                             <  6:368326/137‑1 (MQ=255)
                     tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGGGGAATATTACATGGCCCCCGGGTGTTGCGCCGTTGGCGGCCATTGCcgccggc                                                                                                                                 >  1:308335/1‑148 (MQ=255)
                       cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTg                                                                                                                              <  2:111056/149‑1 (MQ=255)
                       cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGCCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggccca                                                                                                                              <  2:130655/149‑5 (MQ=37)
                         cacaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCCCC‑TTGGGCTCCATTTCCCGCCGGCAGAt                                                                                                                            >  8:305464/1‑149 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                           >  5:26575/1‑149 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                           <  4:27740/149‑1 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                           >  1:5024/1‑149 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggcccagga                                                                                                                           <  8:297639/149‑8 (MQ=37)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggcccagg                                                                                                                            >  7:297639/1‑142 (MQ=37)
                           caGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                          >  3:104679/1‑149 (MQ=255)
                               gATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTa                                                                                                                      >  3:173619/1‑149 (MQ=255)
                                   gCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGcc                                                                                                                  <  4:161034/149‑1 (MQ=255)
                                      gcgcgcCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAg                                                                                                               <  4:6783/149‑1 (MQ=255)
                                          gcCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTGGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACg                                                                                                           <  3:34348/149‑1 (MQ=255)
                                           ccccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACgg                                                                                                          >  6:317714/1‑149 (MQ=255)
                                           ccccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACgg                                                                                                          <  5:339607/149‑1 (MQ=255)
                                            cccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGt                                                                                                         <  3:55148/149‑1 (MQ=255)
                                              cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                       >  5:165181/1‑149 (MQ=255)
                                              cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                       >  8:227144/1‑149 (MQ=255)
                                                 aatcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACg                                                                                                     >  1:347923/1‑148 (MQ=255)
                                                   tcaaAGGACCTGGCCCATCTGCAGCCTGCTCGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑AGGGGCCCCAATTCCGGCCGGCTGAATTGGTTCGCCCACGCGGGTGACg                                                                                                     >  4:698/1‑146 (MQ=255)
                                                      aaGGACCTGGCCCATCTGCAGGCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                               <  4:104679/149‑1 (MQ=255)
                                                      aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                               <  3:156606/149‑1 (MQ=255)
                                                      aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                               <  7:316007/149‑1 (MQ=255)
                                                       aGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                              <  6:26575/149‑1 (MQ=255)
                                                       aGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                              <  8:228542/149‑1 (MQ=255)
                                                         gACCTGGCCCATCTGCAGCCTGCTGGGGGGCTTTGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTg                                                                                            <  7:305464/149‑1 (MQ=255)
                                                          aCCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGa                                                                                           <  5:317714/149‑1 (MQ=255)
                                                                 ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          <  1:122975/143‑1 (MQ=255)
                                                                 ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          >  2:122975/1‑143 (MQ=255)
                                                                  cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                                   <  2:347923/149‑1 (MQ=255)
                                                                     cTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTc                                                                                 >  7:200119/1‑148 (MQ=255)
                                                                      tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGGGCCATTGCCGGCGGGGGGATTTGTTACGCCCAGGACGGGGACGAAGAGTGTGACGGGATTTTCGa                                                                               >  8:212374/1‑149 (MQ=255)
                                                                      tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                                    >  8:93984/1‑128 (MQ=255)
                                                                      tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                                    <  7:93984/128‑1 (MQ=255)
                                                                       gCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAc                                                                              >  6:374773/1‑149 (MQ=255)
                                                                        cAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAcc                                                                             >  7:334458/1‑149 (MQ=255)
                                                                        cAGCCGGCTGGGCGGCTTGGTCGGGGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAc                                                                              <  2:308335/148‑1 (MQ=255)
                                                                                 gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          <  7:258960/127‑1 (MQ=255)
                                                                                 gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          >  8:258960/1‑127 (MQ=255)
                                                                                  ggcggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                   <  8:200119/149‑1 (MQ=255)
                                                                                  ggcggcTTGGGCGGTGGTGGCCATGGGGCCATCGAGAGGTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                   <  7:135020/149‑1 (MQ=255)
                                                                                        ttGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGccc                                                             >  2:75265/1‑149 (MQ=255)
                                                                                            tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCgag                                                         <  5:374773/149‑1 (MQ=255)
                                                                                                     gCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGc                                                 <  8:334458/148‑1 (MQ=255)
                                                                                                           gTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcg                                          >  2:214931/1‑149 (MQ=255)
                                                                                                              ccATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcggcg                                       <  6:165181/149‑1 (MQ=255)
                                                                                                                  cGAGAGTTCTTCCGGGGGCGGCGGTGGTGGCGGC‑TTGGGCGCCTTTGCCGGCGGGCTGATTTGTTTCGCGGAGGACGGTGACGAAGATGGTGACGGCTTTTTCGACCGGGGCGATCAGTGCCCCGAGACCCCTGCCTACACGGCGGt                                     <  4:35508/147‑1 (MQ=255)
                                                                                                                   gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCgg                                                  <  8:321316/133‑1 (MQ=255)
                                                                                                                   gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCgg                                                  >  7:321316/1‑133 (MQ=255)
                                                                                                                    agagTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTgcc                                 >  5:91206/1‑147 (MQ=255)
                                                                                                                     gagTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAcc                                <  1:70136/149‑1 (MQ=255)
                                                                                                                          cttcCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGAGGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                            <  4:173619/148‑1 (MQ=255)
                                                                                                                          cttcCTGGGCCGCCGGTGGCGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATg                           >  6:361334/1‑149 (MQ=255)
                                                                                                                             ccTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATggg                         <  2:5024/148‑1 (MQ=255)
                                                                                                                                 ggCCGCCGGTGGCGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTg                      <  5:361334/147‑1 (MQ=255)
                                                                                                                                   ccgccgGCAATGGCGCC‑CAAGGCGCCACCACCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa                  >  7:58479/1‑149 (MQ=38)
                                                                                                                                    cgccgGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCAc                 >  5:276489/1‑149 (MQ=255)
                                                                                                                                     gccgGTGGTGGCGCC‑TTGGGGGCCATTGCCGGCGGGGTGATTTGTTTCGCCCAGGACGGGGACGAATATGGTGCCGGCATTTTAGACCCGGGCGCTCACAGCCCCGCGACCCCGGCCAACACGGGGGTTTACCACATTGGGTGgccccg                >  6:42109/1‑146 (MQ=255)
                                                                                                                                     gccgGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACt                >  7:78524/1‑149 (MQ=255)
                                                                                                                                        ggtggtGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaa             <  6:276489/149‑1 (MQ=255)
                                                                                                                                         gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCCCTGaaa            >  1:44943/1‑149 (MQ=255)
                                                                                                                                         gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  2:55367/149‑1 (MQ=255)
                                                                                                                                         gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  1:75265/149‑1 (MQ=255)
                                                                                                                                         gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  5:42109/149‑1 (MQ=255)
                                                                                                                                                 gcC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            >  5:144943/1‑141 (MQ=255)
                                                                                                                                                 gcC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  6:144943/141‑1 (MQ=255)
                                                                                                                                                   c‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCgg  >  5:141757/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCGG  >  NC_002947/1284889‑1285184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑