Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,285,038 T→A 7.4% L90* (TTG→TAG) ‡ PP_1122 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,285,0380TA 7.4% 231.8 / 5.6 80L90* (TTG→TAG) ‡PP_1122OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (33/40);  new base A (3/3);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCGG  >  NC_002947/1284891‑1285184
                                                                                                                                                    |                                                                                                                                                  
gCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTg                                                                                                                                                   >  4:34348/1‑149 (MQ=255)
     cTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGGTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGgc                                                                                                                                                <  3:291449/147‑1 (MQ=255)
      tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGgcgc                                                                                                                                              >  7:228542/1‑148 (MQ=255)
        aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAt                                                                                                                                           <  3:224222/149‑1 (MQ=255)
        aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAt                                                                                                                                           >  7:210102/1‑149 (MQ=255)
         cGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAtt                                                                                                                                          <  3:186344/149‑1 (MQ=255)
          gggCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCGTTGGTGCCATCGAGAGTTCTTCATGGGCCGCCGGTGGTGGCGCC‑GTGGGCGCCATTg                                                                                                                                         >  8:135020/1‑149 (MQ=255)
           ggCTGCGCTACGCACAGCTATGGCTGCGCGCCCCTCAATCAAAGGCCCTGGCCCTTCTGCCGCCTTCTGGGGGGATTGGTGGGTGTTGGCCTTGTTGCAATGGATATTTCTTCCTGGGCCGCCTGTGGTGGCGCC‑CAAGGCGCCaccac                                                                                                                                        <  4:185316/149‑5 (MQ=255)
              tGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGCCCGCCGGCAATGGCGCC‑CAAGGCGCCaccaccgg                                                                                                                                     >  1:130655/1‑142 (MQ=38)
                 gcTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCcggcgg                                                                                                                                  <  8:210102/149‑1 (MQ=255)
                  cTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGcc                                                                                                                                             <  6:368326/137‑1 (MQ=255)
                  cTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGcc                                                                                                                                             >  5:368326/1‑137 (MQ=255)
                   tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGGGGAATATTACATGGCCCCCGGGTGTTGCGCCGTTGGCGGCCATTGCcgccggc                                                                                                                                 >  1:308335/1‑148 (MQ=255)
                     cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTg                                                                                                                              <  2:111056/149‑1 (MQ=255)
                     cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGCCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggccca                                                                                                                              <  2:130655/149‑5 (MQ=37)
                       cacaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCCCC‑TTGGGCTCCATTTCCCGCCGGCAGAt                                                                                                                            >  8:305464/1‑149 (MQ=255)
                        acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                           >  5:26575/1‑149 (MQ=255)
                        acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                           >  1:5024/1‑149 (MQ=255)
                        acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                           <  4:27740/149‑1 (MQ=255)
                        acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggcccagga                                                                                                                           <  8:297639/149‑8 (MQ=37)
                        acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggcccagg                                                                                                                            >  7:297639/1‑142 (MQ=37)
                         caGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                          >  3:104679/1‑149 (MQ=255)
                             gATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTa                                                                                                                      >  3:173619/1‑149 (MQ=255)
                                 gCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGcc                                                                                                                  <  4:161034/149‑1 (MQ=255)
                                    gcgcgcCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAg                                                                                                               <  4:6783/149‑1 (MQ=255)
                                        gcCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTGGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACg                                                                                                           <  3:34348/149‑1 (MQ=255)
                                         ccccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACgg                                                                                                          >  6:317714/1‑149 (MQ=255)
                                         ccccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACgg                                                                                                          <  5:339607/149‑1 (MQ=255)
                                          cccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGt                                                                                                         <  3:55148/149‑1 (MQ=255)
                                            cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                       >  8:227144/1‑149 (MQ=255)
                                            cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                       >  5:165181/1‑149 (MQ=255)
                                               aatcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACg                                                                                                     >  1:347923/1‑148 (MQ=255)
                                                 tcaaAGGACCTGGCCCATCTGCAGCCTGCTCGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑AGGGGCCCCAATTCCGGCCGGCTGAATTGGTTCGCCCACGCGGGTGACg                                                                                                     >  4:698/1‑146 (MQ=255)
                                                    aaGGACCTGGCCCATCTGCAGGCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                               <  4:104679/149‑1 (MQ=255)
                                                    aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                               <  3:156606/149‑1 (MQ=255)
                                                    aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                               <  7:316007/149‑1 (MQ=255)
                                                     aGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                              <  6:26575/149‑1 (MQ=255)
                                                     aGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                              <  8:228542/149‑1 (MQ=255)
                                                       gACCTGGCCCATCTGCAGCCTGCTGGGGGGCTTTGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTg                                                                                            <  7:305464/149‑1 (MQ=255)
                                                        aCCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGa                                                                                           <  5:317714/149‑1 (MQ=255)
                                                               ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          <  1:122975/143‑1 (MQ=255)
                                                               ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          >  2:122975/1‑143 (MQ=255)
                                                                cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                                   <  2:347923/149‑1 (MQ=255)
                                                                   cTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTc                                                                                 >  7:200119/1‑148 (MQ=255)
                                                                    tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGGGCCATTGCCGGCGGGGGGATTTGTTACGCCCAGGACGGGGACGAAGAGTGTGACGGGATTTTCGa                                                                               >  8:212374/1‑149 (MQ=255)
                                                                    tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                                    <  7:93984/128‑1 (MQ=255)
                                                                    tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                                    >  8:93984/1‑128 (MQ=255)
                                                                     gCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAc                                                                              >  6:374773/1‑149 (MQ=255)
                                                                      cAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAcc                                                                             >  7:334458/1‑149 (MQ=255)
                                                                      cAGCCGGCTGGGCGGCTTGGTCGGGGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAc                                                                              <  2:308335/148‑1 (MQ=255)
                                                                               gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          <  7:258960/127‑1 (MQ=255)
                                                                               gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                          >  8:258960/1‑127 (MQ=255)
                                                                                ggcggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                   <  8:200119/149‑1 (MQ=255)
                                                                                ggcggcTTGGGCGGTGGTGGCCATGGGGCCATCGAGAGGTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                   <  7:135020/149‑1 (MQ=255)
                                                                                      ttGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGccc                                                             >  2:75265/1‑149 (MQ=255)
                                                                                          tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCgag                                                         <  5:374773/149‑1 (MQ=255)
                                                                                                   gCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGc                                                 <  8:334458/148‑1 (MQ=255)
                                                                                                         gTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcg                                          >  2:214931/1‑149 (MQ=255)
                                                                                                            ccATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcggcg                                       <  6:165181/149‑1 (MQ=255)
                                                                                                                cGAGAGTTCTTCCGGGGGCGGCGGTGGTGGCGGC‑TTGGGCGCCTTTGCCGGCGGGCTGATTTGTTTCGCGGAGGACGGTGACGAAGATGGTGACGGCTTTTTCGACCGGGGCGATCAGTGCCCCGAGACCCCTGCCTACACGGCGGt                                     <  4:35508/147‑1 (MQ=255)
                                                                                                                 gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCgg                                                  <  8:321316/133‑1 (MQ=255)
                                                                                                                 gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCgg                                                  >  7:321316/1‑133 (MQ=255)
                                                                                                                  agagTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTgcc                                 >  5:91206/1‑147 (MQ=255)
                                                                                                                   gagTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAcc                                <  1:70136/149‑1 (MQ=255)
                                                                                                                        cttcCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGAGGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                            <  4:173619/148‑1 (MQ=255)
                                                                                                                        cttcCTGGGCCGCCGGTGGCGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATg                           >  6:361334/1‑149 (MQ=255)
                                                                                                                           ccTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATggg                         <  2:5024/148‑1 (MQ=255)
                                                                                                                               ggCCGCCGGTGGCGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTg                      <  5:361334/147‑1 (MQ=255)
                                                                                                                                 ccgccgGCAATGGCGCC‑CAAGGCGCCACCACCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa                  >  7:58479/1‑149 (MQ=38)
                                                                                                                                  cgccgGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCAc                 >  5:276489/1‑149 (MQ=255)
                                                                                                                                   gccgGTGGTGGCGCC‑TTGGGGGCCATTGCCGGCGGGGTGATTTGTTTCGCCCAGGACGGGGACGAATATGGTGCCGGCATTTTAGACCCGGGCGCTCACAGCCCCGCGACCCCGGCCAACACGGGGGTTTACCACATTGGGTGgccccg                >  6:42109/1‑146 (MQ=255)
                                                                                                                                   gccgGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACt                >  7:78524/1‑149 (MQ=255)
                                                                                                                                      ggtggtGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaa             <  6:276489/149‑1 (MQ=255)
                                                                                                                                       gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCCCTGaaa            >  1:44943/1‑149 (MQ=255)
                                                                                                                                       gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  1:75265/149‑1 (MQ=255)
                                                                                                                                       gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  2:55367/149‑1 (MQ=255)
                                                                                                                                       gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  5:42109/149‑1 (MQ=255)
                                                                                                                                               gcC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            >  5:144943/1‑141 (MQ=255)
                                                                                                                                               gcC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa            <  6:144943/141‑1 (MQ=255)
                                                                                                                                                 c‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCgg  >  5:141757/1‑149 (MQ=255)
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GCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCGG  >  NC_002947/1284891‑1285184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑