Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,285,047 T→C 5.6% I93T (ATT→ACT)  PP_1122 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,285,0470TC 5.6% 220.3 / 2.1 72I93T (ATT→ACT) PP_1122OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (29/38);  new base C (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCGGCCGCGCCAC  >  NC_002947/1284899‑1285193
                                                                                                                                                     |                                                                                                                                                  
aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAt                                                                                                                                                    >  7:210102/1‑149 (MQ=255)
aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAt                                                                                                                                                    <  3:224222/149‑1 (MQ=255)
 cGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCAtt                                                                                                                                                   <  3:186344/149‑1 (MQ=255)
  gggCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCGTTGGTGCCATCGAGAGTTCTTCATGGGCCGCCGGTGGTGGCGCC‑GTGGGCGCCATTg                                                                                                                                                  >  8:135020/1‑149 (MQ=255)
         gcTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCcggcgg                                                                                                                                           <  8:210102/149‑1 (MQ=255)
           tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGGGGAATATTACATGGCCCCCGGGTGTTGCGCCGTTGGCGGCCATTGCcgccggc                                                                                                                                          >  1:308335/1‑148 (MQ=255)
             cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTg                                                                                                                                       <  2:111056/149‑1 (MQ=255)
             cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGCCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggccca                                                                                                                                       <  2:130655/149‑5 (MQ=37)
               cacaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCCCC‑TTGGGCTCCATTTCCCGCCGGCAGAt                                                                                                                                     >  8:305464/1‑149 (MQ=255)
                acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                                    <  4:27740/149‑1 (MQ=255)
                acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                                    >  5:26575/1‑149 (MQ=255)
                acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                                    >  1:5024/1‑149 (MQ=255)
                acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggcccagga                                                                                                                                    <  8:297639/149‑8 (MQ=37)
                acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCC‑CAAGGCGCCACCACcggcggcccagg                                                                                                                                     >  7:297639/1‑142 (MQ=37)
                 caGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                                   >  3:104679/1‑149 (MQ=255)
                     gATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTa                                                                                                                               >  3:173619/1‑149 (MQ=255)
                         gCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGcc                                                                                                                           <  4:161034/149‑1 (MQ=255)
                            gcgcgcCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAg                                                                                                                        <  4:6783/149‑1 (MQ=255)
                                gcCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTGGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACg                                                                                                                    <  3:34348/149‑1 (MQ=255)
                                 ccccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACgg                                                                                                                   <  5:339607/149‑1 (MQ=255)
                                 ccccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACgg                                                                                                                   >  6:317714/1‑149 (MQ=255)
                                  cccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGt                                                                                                                  <  3:55148/149‑1 (MQ=255)
                                    cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                                >  5:165181/1‑149 (MQ=255)
                                    cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                                >  8:227144/1‑149 (MQ=255)
                                       aatcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACg                                                                                                              >  1:347923/1‑148 (MQ=255)
                                         tcaaAGGACCTGGCCCATCTGCAGCCTGCTCGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑AGGGGCCCCAATTCCGGCCGGCTGAATTGGTTCGCCCACGCGGGTGACg                                                                                                              >  4:698/1‑146 (MQ=255)
                                            aaGGACCTGGCCCATCTGCAGGCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                                        <  4:104679/149‑1 (MQ=255)
                                            aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                                        <  3:156606/149‑1 (MQ=255)
                                            aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                                        <  7:316007/149‑1 (MQ=255)
                                             aGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                                       <  6:26575/149‑1 (MQ=255)
                                             aGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                                       <  8:228542/149‑1 (MQ=255)
                                               gACCTGGCCCATCTGCAGCCTGCTGGGGGGCTTTGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTg                                                                                                     <  7:305464/149‑1 (MQ=255)
                                                aCCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGa                                                                                                    <  5:317714/149‑1 (MQ=255)
                                                       ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                                   >  2:122975/1‑143 (MQ=255)
                                                       ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                                   <  1:122975/143‑1 (MQ=255)
                                                        cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                                            <  2:347923/149‑1 (MQ=255)
                                                           cTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTc                                                                                          >  7:200119/1‑148 (MQ=255)
                                                            tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGGGCCATTGCCGGCGGGGGGATTTGTTACGCCCAGGACGGGGACGAAGAGTGTGACGGGATTTTCGa                                                                                        >  8:212374/1‑149 (MQ=255)
                                                            tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                                             <  7:93984/128‑1 (MQ=255)
                                                            tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                                             >  8:93984/1‑128 (MQ=255)
                                                             gCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAc                                                                                       >  6:374773/1‑149 (MQ=255)
                                                              cAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAcc                                                                                      >  7:334458/1‑149 (MQ=255)
                                                              cAGCCGGCTGGGCGGCTTGGTCGGGGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAc                                                                                       <  2:308335/148‑1 (MQ=255)
                                                                       gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                                   <  7:258960/127‑1 (MQ=255)
                                                                       gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGAc                                                                                                   >  8:258960/1‑127 (MQ=255)
                                                                        ggcggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                            <  8:200119/149‑1 (MQ=255)
                                                                        ggcggcTTGGGCGGTGGTGGCCATGGGGCCATCGAGAGGTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                            <  7:135020/149‑1 (MQ=255)
                                                                              ttGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGccc                                                                      >  2:75265/1‑149 (MQ=255)
                                                                                  tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCgag                                                                  <  5:374773/149‑1 (MQ=255)
                                                                                           gCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGc                                                          <  8:334458/148‑1 (MQ=255)
                                                                                                 gTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcg                                                   >  2:214931/1‑149 (MQ=255)
                                                                                                    ccATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcggcg                                                <  6:165181/149‑1 (MQ=255)
                                                                                                        cGAGAGTTCTTCCGGGGGCGGCGGTGGTGGCGGC‑TTGGGCGCCTTTGCCGGCGGGCTGATTTGTTTCGCGGAGGACGGTGACGAAGATGGTGACGGCTTTTTCGACCGGGGCGATCAGTGCCCCGAGACCCCTGCCTACACGGCGGt                                              <  4:35508/147‑1 (MQ=255)
                                                                                                         gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCgg                                                           >  7:321316/1‑133 (MQ=255)
                                                                                                         gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCgg                                                           <  8:321316/133‑1 (MQ=255)
                                                                                                          agagTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTgcc                                          >  5:91206/1‑147 (MQ=255)
                                                                                                           gagTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAcc                                         <  1:70136/149‑1 (MQ=255)
                                                                                                                cttcCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGAGGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                                     <  4:173619/148‑1 (MQ=255)
                                                                                                                cttcCTGGGCCGCCGGTGGCGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATg                                    >  6:361334/1‑149 (MQ=255)
                                                                                                                   ccTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATggg                                  <  2:5024/148‑1 (MQ=255)
                                                                                                                       ggCCGCCGGTGGCGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTg                               <  5:361334/147‑1 (MQ=255)
                                                                                                                         ccgccgGCAATGGCGCC‑CAAGGCGCCACCACCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa                           >  7:58479/1‑149 (MQ=38)
                                                                                                                          cgccgGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCAc                          >  5:276489/1‑149 (MQ=255)
                                                                                                                           gccgGTGGTGGCGCC‑TTGGGGGCCATTGCCGGCGGGGTGATTTGTTTCGCCCAGGACGGGGACGAATATGGTGCCGGCATTTTAGACCCGGGCGCTCACAGCCCCGCGACCCCGGCCAACACGGGGGTTTACCACATTGGGTGgccccg                         >  6:42109/1‑146 (MQ=255)
                                                                                                                           gccgGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACt                         >  7:78524/1‑149 (MQ=255)
                                                                                                                              ggtggtGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaa                      <  6:276489/149‑1 (MQ=255)
                                                                                                                               gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCCCTGaaa                     >  1:44943/1‑149 (MQ=255)
                                                                                                                               gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa                     <  5:42109/149‑1 (MQ=255)
                                                                                                                               gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa                     <  2:55367/149‑1 (MQ=255)
                                                                                                                               gtggtgGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa                     <  1:75265/149‑1 (MQ=255)
                                                                                                                                       gcC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa                     <  6:144943/141‑1 (MQ=255)
                                                                                                                                       gcC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa                     >  5:144943/1‑141 (MQ=255)
                                                                                                                                         c‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCgg           >  5:141757/1‑149 (MQ=255)
                                                                                                                                             gggCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCAGATGGGCTGCCCACTGAAACAGTACCCGGcc         <  1:30729/148‑1 (MQ=255)
                                                                                                                                                   cATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCGGCCGCGCCAc  <  7:227144/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCGGCCGCGCCAC  >  NC_002947/1284899‑1285193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑