Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,294,114 C→T 7.7% intergenic (+15/+25) pdxH → / ← PP_1130 pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,294,1140CT 7.7% 187.5 / 3.6 64intergenic (+15/+25)pdxH/PP_1130pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/31);  new base T (2/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

TGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCC  >  NC_002947/1293967‑1294261
                                                                                                                                                   |                                                                                                                                                     
tGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGgccgcc                                                                                                                                                       >  3:211013/1‑148 (MQ=255)
 gAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCaa                                                                                                                                                     >  8:44960/1‑149 (MQ=255)
   gCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGc                                                                                                                                                   <  3:288602/149‑1 (MQ=255)
     aTTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCgg                                                                                                                                                 >  2:320721/1‑149 (MQ=255)
      ttGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGGGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGc                                                                                                                                                <  5:352492/149‑1 (MQ=255)
           ggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAg                                                                                                                                           <  1:232542/149‑1 (MQ=255)
           ggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAg                                                                                                                                           <  3:340292/149‑1 (MQ=255)
           ggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAg                                                                                                                                           <  8:42286/149‑1 (MQ=255)
            gggATACCGTTTGTTGCCCGAGCGAATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGCCGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGc                                                                                                                                          <  3:2508/149‑1 (MQ=255)
             ggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGc                                                                                                                                          >  7:203133/1‑148 (MQ=255)
                 aCCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                                      >  6:222583/1‑148 (MQ=255)
                  ccGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTg                                                                                                                                     <  4:211013/148‑1 (MQ=255)
                  ccGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGa                                                                                                                                    >  3:13569/1‑149 (MQ=255)
                  ccGTTTGTGGCCCGAGCGCATCGAGTGCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTTCGAGAGCGTCTGGCGCCCTGAGCAACTGGTGCCGCCAAGCGGCCCCAGCCATTGa                                                                                                                                    <  3:164115/149‑1 (MQ=255)
                   cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTg                                                                                                                                     >  8:153122/1‑147 (MQ=255)
                   cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTg                                                                                                                                     <  7:153122/147‑1 (MQ=255)
                   cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGAt                                                                                                                                   >  8:185579/1‑149 (MQ=255)
                        gttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                                      <  5:252426/141‑1 (MQ=255)
                        gttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                                      >  6:252426/1‑141 (MQ=255)
                         ttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGc                                                                                                                              <  5:222583/148‑1 (MQ=255)
                            cccGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCAGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGATAcc                                                                                                                          >  4:56810/1‑149 (MQ=255)
                              cGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTAcccc                                                                                                                        <  7:44960/149‑1 (MQ=255)
                                 gcgcATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGa                                                                                                                     <  2:234512/149‑1 (MQ=255)
                                      tCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑cc                                                                                                               <  2:200571/149‑1 (MQ=255)
                                        gAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCaa                                                                                                                                <  6:216412/131‑1 (MQ=255)
                                        gAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCaa                                                                                                                                >  5:216412/1‑131 (MQ=255)
                                         agggcTGGCAGGGGCGGGCAAGTCGTCTGCAGGATCGTTTGAAGTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTgc                                                                                                            <  8:271829/145‑1 (MQ=255)
                                              tGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCt                                                                                                       >  8:243122/1‑149 (MQ=255)
                                                 cAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcgc                                                                                                    <  2:128121/149‑1 (MQ=255)
                                                  aGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcgcg                                                                                                   >  3:286128/1‑149 (MQ=255)
                                                     ggcgggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCTGATAA‑CCTGCCGCCTCGCGCTc                                                                                                <  1:356099/149‑1 (MQ=255)
                                                         ggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGACCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAac                                                                                            <  7:243122/149‑1 (MQ=255)
                                                           cAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTTCCCCGGATAA‑CCTGCCGCCTCGCGCTCCAacca                                                                                          <  8:203133/149‑1 (MQ=255)
                                                              gccgtcTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAaccac                                                                                         <  1:320721/145‑1 (MQ=255)
                                                                 gtcTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑Gc                                                                                   >  2:280084/1‑149 (MQ=255)
                                                                       aTGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTg                                                                             <  7:185579/149‑1 (MQ=255)
                                                                        tGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑Gc                                                                                   <  4:195560/142‑1 (MQ=255)
                                                                        tGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑Gc                                                                                   >  3:195560/1‑142 (MQ=255)
                                                                            cGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTa                                                                         <  4:13569/148‑1 (MQ=255)
                                                                             gTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACg                                                                       <  1:52723/149‑1 (MQ=255)
                                                                                   aCTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCAc                                                                 >  4:142381/1‑149 (MQ=255)
                                                                                     tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCt                                                               >  7:146194/1‑149 (MQ=255)
                                                                                         ggCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTtc                                                             <  1:280084/148‑1 (MQ=255)
                                                                                          gCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcg                                                                                                     <  4:363049/107‑1 (MQ=255)
                                                                                          gCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcg                                                                                                     >  3:363049/1‑107 (MQ=255)
                                                                                              gtggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGcc                                                      >  5:155012/1‑149 (MQ=255)
                                                                                                            gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCaa                                         <  1:272834/148‑1 (MQ=255)
                                                                                                            gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAc                                        <  8:146194/149‑1 (MQ=255)
                                                                                                              tGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCt                                      >  8:139368/1‑149 (MQ=255)
                                                                                                                  agagCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTcc                                  <  7:139368/149‑1 (MQ=255)
                                                                                                                  agagCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTc                                   >  1:259706/1‑148 (MQ=255)
                                                                                                                   gagCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGAGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCATGCCGCGCCTGCGCCACCCTTTc                                   >  2:278516/1‑147 (MQ=255)
                                                                                                                       ggCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                             >  5:101938/1‑149 (MQ=255)
                                                                                                                            gcgcCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAc                         <  8:106196/148‑1 (MQ=255)
                                                                                                                              gcCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcg                      >  6:250408/1‑149 (MQ=255)
                                                                                                                              gcCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATAAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcg                      >  8:5479/1‑149 (MQ=255)
                                                                                                                                       aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGa                                                                                                                     <  1:310972/47‑1 (MQ=255)
                                                                                                                                       aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGa                                                                                                                     >  2:310972/1‑47 (MQ=255)
                                                                                                                                       aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcgc                     <  6:232922/141‑1 (MQ=255)
                                                                                                                                       aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcgc                     >  5:232922/1‑141 (MQ=255)
                                                                                                                                         cTGGGGCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg            >  4:312822/1‑148 (MQ=255)
                                                                                                                                          tGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGGCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGa          >  8:1875/1‑149 (MQ=255)
                                                                                                                                           ggggCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGAt         >  4:266741/1‑149 (MQ=255)
                                                                                                                                            gggCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATc        >  5:143492/1‑149 (MQ=255)
                                                                                                                                             ggCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCg       <  3:266741/149‑1 (MQ=255)
                                                                                                                                              gccgccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCgg      >  4:50274/1‑149 (MQ=255)
                                                                                                                                               ccgccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGTCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGc     <  4:92796/149‑1 (MQ=255)
                                                                                                                                                   cAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCCTTCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGcccc  >  1:298192/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCC  >  NC_002947/1293967‑1294261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑