Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,294,115 A→T 7.4% intergenic (+16/+24) pdxH → / ← PP_1130 pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,294,1150AT 7.4% 194.6 / 3.6 66intergenic (+16/+24)pdxH/PP_1130pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/32);  new base T (2/3);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCC  >  NC_002947/1293968‑1294261
                                                                                                                                                   |                                                                                                                                                    
gAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCaa                                                                                                                                                     >  8:44960/1‑149 (MQ=255)
  gCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGc                                                                                                                                                   <  3:288602/149‑1 (MQ=255)
    aTTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCgg                                                                                                                                                 >  2:320721/1‑149 (MQ=255)
     ttGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGGGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGc                                                                                                                                                <  5:352492/149‑1 (MQ=255)
          ggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAg                                                                                                                                           <  1:232542/149‑1 (MQ=255)
          ggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAg                                                                                                                                           <  3:340292/149‑1 (MQ=255)
          ggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAg                                                                                                                                           <  8:42286/149‑1 (MQ=255)
           gggATACCGTTTGTTGCCCGAGCGAATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGCCGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGc                                                                                                                                          <  3:2508/149‑1 (MQ=255)
            ggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGc                                                                                                                                          >  7:203133/1‑148 (MQ=255)
                aCCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                                      >  6:222583/1‑148 (MQ=255)
                 ccGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTg                                                                                                                                     <  4:211013/148‑1 (MQ=255)
                 ccGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGa                                                                                                                                    >  3:13569/1‑149 (MQ=255)
                 ccGTTTGTGGCCCGAGCGCATCGAGTGCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTTCGAGAGCGTCTGGCGCCCTGAGCAACTGGTGCCGCCAAGCGGCCCCAGCCATTGa                                                                                                                                    <  3:164115/149‑1 (MQ=255)
                  cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTg                                                                                                                                     >  8:153122/1‑147 (MQ=255)
                  cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTg                                                                                                                                     <  7:153122/147‑1 (MQ=255)
                  cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGAt                                                                                                                                   >  8:185579/1‑149 (MQ=255)
                       gttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                                      <  5:252426/141‑1 (MQ=255)
                       gttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                                      >  6:252426/1‑141 (MQ=255)
                        ttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGc                                                                                                                              <  5:222583/148‑1 (MQ=255)
                           cccGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCAGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGATAcc                                                                                                                          >  4:56810/1‑149 (MQ=255)
                             cGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTAcccc                                                                                                                        <  7:44960/149‑1 (MQ=255)
                                gcgcATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGa                                                                                                                     <  2:234512/149‑1 (MQ=255)
                                     tCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑cc                                                                                                               <  2:200571/149‑1 (MQ=255)
                                       gAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCaa                                                                                                                                >  5:216412/1‑131 (MQ=255)
                                       gAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCaa                                                                                                                                <  6:216412/131‑1 (MQ=255)
                                        agggcTGGCAGGGGCGGGCAAGTCGTCTGCAGGATCGTTTGAAGTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTgc                                                                                                            <  8:271829/145‑1 (MQ=255)
                                             tGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCt                                                                                                       >  8:243122/1‑149 (MQ=255)
                                                cAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcgc                                                                                                    <  2:128121/149‑1 (MQ=255)
                                                 aGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcgcg                                                                                                   >  3:286128/1‑149 (MQ=255)
                                                    ggcgggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCTGATAA‑CCTGCCGCCTCGCGCTc                                                                                                <  1:356099/149‑1 (MQ=255)
                                                        ggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGACCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAac                                                                                            <  7:243122/149‑1 (MQ=255)
                                                          cAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTTCCCCGGATAA‑CCTGCCGCCTCGCGCTCCAacca                                                                                          <  8:203133/149‑1 (MQ=255)
                                                             gccgtcTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAaccac                                                                                         <  1:320721/145‑1 (MQ=255)
                                                                gtcTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑Gc                                                                                   >  2:280084/1‑149 (MQ=255)
                                                                      aTGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTg                                                                             <  7:185579/149‑1 (MQ=255)
                                                                       tGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑Gc                                                                                   <  4:195560/142‑1 (MQ=255)
                                                                       tGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑Gc                                                                                   >  3:195560/1‑142 (MQ=255)
                                                                           cGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTa                                                                         <  4:13569/148‑1 (MQ=255)
                                                                            gTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACg                                                                       <  1:52723/149‑1 (MQ=255)
                                                                                  aCTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCAc                                                                 >  4:142381/1‑149 (MQ=255)
                                                                                    tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCt                                                               >  7:146194/1‑149 (MQ=255)
                                                                                        ggCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTtc                                                             <  1:280084/148‑1 (MQ=255)
                                                                                         gCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcg                                                                                                     <  4:363049/107‑1 (MQ=255)
                                                                                         gCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTcg                                                                                                     >  3:363049/1‑107 (MQ=255)
                                                                                             gtggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGcc                                                      >  5:155012/1‑149 (MQ=255)
                                                                                                           gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCaa                                         <  1:272834/148‑1 (MQ=255)
                                                                                                           gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAc                                        <  8:146194/149‑1 (MQ=255)
                                                                                                             tGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCt                                      >  8:139368/1‑149 (MQ=255)
                                                                                                                 agagCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTcc                                  <  7:139368/149‑1 (MQ=255)
                                                                                                                 agagCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTc                                   >  1:259706/1‑148 (MQ=255)
                                                                                                                  gagCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGAGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCATGCCGCGCCTGCGCCACCCTTTc                                   >  2:278516/1‑147 (MQ=255)
                                                                                                                      ggCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                             >  5:101938/1‑149 (MQ=255)
                                                                                                                           gcgcCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAc                         <  8:106196/148‑1 (MQ=255)
                                                                                                                             gcCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcg                      >  6:250408/1‑149 (MQ=255)
                                                                                                                             gcCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATAAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcg                      >  8:5479/1‑149 (MQ=255)
                                                                                                                                      aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGa                                                                                                                     <  1:310972/47‑1 (MQ=255)
                                                                                                                                      aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGa                                                                                                                     >  2:310972/1‑47 (MQ=255)
                                                                                                                                      aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcgc                     <  6:232922/141‑1 (MQ=255)
                                                                                                                                      aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcgc                     >  5:232922/1‑141 (MQ=255)
                                                                                                                                        cTGGGGCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg            >  4:312822/1‑148 (MQ=255)
                                                                                                                                         tGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGGCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGa          >  8:1875/1‑149 (MQ=255)
                                                                                                                                          ggggCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGAt         >  4:266741/1‑149 (MQ=255)
                                                                                                                                           gggCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATc        >  5:143492/1‑149 (MQ=255)
                                                                                                                                            ggCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCg       <  3:266741/149‑1 (MQ=255)
                                                                                                                                             gccgccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCgg      >  4:50274/1‑149 (MQ=255)
                                                                                                                                              ccgccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGTCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGc     <  4:92796/149‑1 (MQ=255)
                                                                                                                                                  cAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCCTTCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGcccc  >  1:298192/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAA‑CCTGCCGCCTCGCGCTCCAACCACCCC‑GCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCC  >  NC_002947/1293968‑1294261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑