Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	150,829	G→T	6.8%	L41M (CTG→ATG) ‡	PP_0142 ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	150,829	0	G	T	6.8%	264.0 / 6.6	83	L41M (CTG→ATG) ‡	PP_0142	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (34/43);  new base T (3/3);  total (37/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

TGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAGG  >  NC_002947/150687‑150976                                                                                                                                               |                                                                                                                                                    tggctggcTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGgcgc                                                                                                                                                 >  1:299818/1‑147 (MQ=255) tggctggcTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGgcgc                                                                                                                                                 <  2:299818/147‑1 (MQ=255)     tggctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCCTGcgctcgcat                                                                                                                                           <  2:373122/149‑3 (MQ=255)       gctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCCTGcgctcgcatt                                                                                                                                          <  1:340993/148‑4 (MQ=255)       gctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCtctc                                                                                                                                         <  5:224296/149‑1 (MQ=255)       gctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCtctc                                                                                                                                         <  6:169461/149‑1 (MQ=255)         ttCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGctct                                                                                                                                          >  5:47193/1‑146 (MQ=255)         ttCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGctct                                                                                                                                          <  6:47193/146‑1 (MQ=255)         ttCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGCGCAATGCGAGCGCAGGCGCTCGCTCTcgc                                                                                                                                       >  8:298480/1‑149 (MQ=255)           cAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCgcgc                                                                                                                                     >  8:306188/1‑149 (MQ=255)            aGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCgcgc                                                                                                                                     <  3:239183/148‑1 (MQ=255)            aGGCAGCGCGTCGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCt                                                                                                                                    >  7:8109/1‑149 (MQ=255)               cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGa                                                                                                                                 <  6:341599/149‑1 (MQ=255)               cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGa                                                                                                                                 <  8:278756/149‑1 (MQ=255)               cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGa                                                                                                                                 <  5:183254/149‑1 (MQ=255)                      tGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCATGCGCTCGCTCTCGCTCTTGAGGTTgtc                                                                                                                          <  6:171100/149‑3 (MQ=255)                      tGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGc                                                                                                                          <  2:156765/149‑1 (MQ=255)                      tGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGc                                                                                                                          <  7:74407/149‑1 (MQ=255)                         gTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCg                                                                                                                         >  7:117188/1‑147 (MQ=255)                          tGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAg                                                                                                                      >  6:342780/1‑149 (MQ=255)                          tGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGGGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAg                                                                                                                      >  5:80733/1‑149 (MQ=255)                              gTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGtgtg                                                                                                                  >  8:185941/1‑149 (MQ=255)                              gTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGtgtg                                                                                                                  >  6:232205/1‑149 (MQ=255)                               tGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGc                                                                                                                 >  6:112838/1‑149 (MQ=255)                               tGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGc                                                                                                                 <  4:67828/149‑1 (MQ=255)                                  gACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAg                                                                                                              >  2:68345/1‑149 (MQ=255)                                  gACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTAGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAg                                                                                                              >  6:123293/1‑149 (MQ=255)                                       gcgcTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTc                                                                                                          >  2:129123/1‑148 (MQ=255)                                         gcTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAg                                                                                                       <  1:175918/149‑1 (MQ=255)                                             ggtgCCTAGGAGTTCGGCCAGGAGCGATGCGCCGGCAGTGTCCAGGCGGCCCAGCTTGCTTAGGTCGGCGACGGTGTCGGCAGTGCAATGCTAGCGCAGTCGCTCGCTCTCGCGCTTGATGTTGGCGTAGTGTGGCAGGGTCCAGTCAc                                                                                                   <  1:182347/146‑1 (MQ=255)                                             ggAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAc                                                                                                   <  8:117188/149‑1 (MQ=255)                                             ggAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAc                                                                                                   <  1:190260/149‑1 (MQ=255)                                               aGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCg                                                                                                 <  3:57797/149‑1 (MQ=255)                                                 ccTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCgg                                                                                                >  5:13661/1‑148 (MQ=255)                                                 ccTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTAGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCgg                                                                                                >  8:187646/1‑148 (MQ=255)                                                   tAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTaa                                                                                             >  4:348965/1‑149 (MQ=255)                                                           cGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcag                                                                                      <  3:61742/148‑1 (MQ=255)                                                           cGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcag                                                                                      <  2:165869/148‑1 (MQ=255)                                                            ggCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcaggc                                                                                    <  7:187646/149‑1 (MQ=255)                                                               cagcagCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGcaggcagg                                                                                 >  7:210241/1‑149 (MQ=255)                                                               cagcagAGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGTCTGAGGTCGGCGAAGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCTCTCTAGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTACATTCACCGGTAATACGcaggcagg                                                                                 >  6:102531/1‑149 (MQ=255)                                                                agcagcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                <  8:8109/149‑1 (MQ=255)                                                                agcagcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                >  5:134632/1‑149 (MQ=255)                                                                 gcagcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGcc                                                                               <  1:68345/149‑1 (MQ=255)                                                                     cGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGtt                                                                           >  3:179022/1‑149 (MQ=255)                                                                            ccGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgctgct                                                                    >  5:151067/1‑149 (MQ=255)                                                                             cGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGtt                                                                           >  6:104858/1‑141 (MQ=255)                                                                             cGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGtt                                                                           <  5:104858/141‑1 (MQ=255)                                                                             cGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGctgctg                                                                   <  4:212605/149‑1 (MQ=255)                                                                                cAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGgtg                                                                >  2:105877/1‑149 (MQ=255)                                                                                 aGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                >  7:258329/1‑132 (MQ=255)                                                                                 aGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                                <  8:258329/132‑1 (MQ=255)                                                                                   tgtCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTcc                                                             <  1:24873/149‑1 (MQ=255)                                                                                     tCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAg                                                           >  3:16359/1‑149 (MQ=255)                                                                                     tCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAg                                                           >  6:271870/1‑149 (MQ=255)                                                                                       cAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAgg                                                          <  1:147791/148‑1 (MQ=255)                                                                                        aGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGt                                                        <  8:210241/149‑1 (MQ=255)                                                                                        aGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGt                                                        <  5:271870/149‑1 (MQ=255)                                                                                           cggcCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGGCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGgc                                                     <  1:94413/149‑1 (MQ=255)                                                                                              cccAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGCGGCGCt                                                  <  5:112838/149‑1 (MQ=255)                                                                                                cAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTgg                                                <  6:236021/149‑1 (MQ=255)                                                                                                 aGCTGGCTGAGGTCTGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTggg                                               <  5:232205/149‑1 (MQ=255)                                                                                                      gctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          <  3:348965/149‑1 (MQ=255)                                                                                                      gctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          <  4:179022/149‑1 (MQ=255)                                                                                                          aGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTc                                        <  6:237435/147‑1 (MQ=255)                                                                                                           ggTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCa                                       >  7:45345/1‑147 (MQ=255)                                                                                                             tCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          >  4:254548/1‑142 (MQ=255)                                                                                                             tCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                                          <  3:254548/142‑1 (MQ=255)                                                                                                                gCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTc                                >  6:315583/1‑149 (MQ=255)                                                                                                                 cGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTcc                               <  5:342780/149‑1 (MQ=255)                                                                                                                       tgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCaa                         <  6:151067/149‑1 (MQ=255)                                                                                                                          cGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGgtgt                                                               >  1:14446/1‑108 (MQ=255)                                                                                                                          cGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGgtgt                                                               <  2:14446/108‑1 (MQ=255)                                                                                                                           ggCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGt                                                                            >  2:312634/1‑94 (MQ=255)                                                                                                                              aGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTc                  >  2:284579/1‑149 (MQ=255)                                                                                                                              aGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTc                  <  5:123293/149‑1 (MQ=255)                                                                                                                                ggCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCgg                >  3:298013/1‑149 (MQ=255)                                                                                                                                  gagagCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGgcg              >  3:5812/5‑149 (MQ=255)                                                                                                                                    gagCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGgcgc             >  1:27984/3‑148 (MQ=255)                                                                                                                                     agCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCGAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCt            >  2:262627/2‑148 (MQ=255)                                                                                                                                       cGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAg         <  7:298480/149‑1 (MQ=255)                                                                                                                                             cAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAg   <  4:16359/149‑1 (MQ=255)                                                                                                                                              aGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAgg  <  8:45345/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    TGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTAGG  >  NC_002947/150687‑150976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑