Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,310,130	A→T	5.6%	L468Q (CTG→CAG)	PP_1144 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,310,130	0	A	T	5.6%	296.6 / 2.6	95	L468Q (CTG→CAG)	PP_1144	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (51/38);  new base T (3/3);  total (54/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGTGCTGCCATCGTCCGG  >  NC_002947/1309983‑1310278                                                                                                                                                    |                                                                                                                                                      ccGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCa                                                                                                                                                       <  3:5308/148‑1 (MQ=255)   gACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGct                                                                                                                                                    >  4:317551/1‑149 (MQ=255)    aCAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGctc                                                                                                                                                   >  1:154857/1‑149 (MQ=255)      aaaaCGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGctct                                                                                                                                                  >  3:357901/1‑148 (MQ=255)      aaaaCGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCtctt                                                                                                                                                 >  4:204773/1‑149 (MQ=255)       aaaCGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCTGCGCCTGGCGCTGctcgaa                                                                                                                                                <  8:197802/149‑4 (MQ=255)       aaaCGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCtcttc                                                                                                                                                <  4:357901/149‑1 (MQ=255)          cGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCttcttc                                                                                                                                             >  6:215705/1‑149 (MQ=255)           ggTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCttcttc                                                                                                                                             >  6:153739/1‑148 (MQ=255)               gggCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGTCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGtt                                                                                                                                        <  2:13525/149‑1 (MQ=255)               gggCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGtt                                                                                                                                        <  5:215705/149‑1 (MQ=255)               gggCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGt                                                                                                                                         <  2:142701/148‑1 (MQ=255)                 gCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTg                                                                                                                                       >  4:102152/1‑148 (MQ=255)                   ccGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGc                                                                                                                                      >  5:41292/1‑147 (MQ=255)                    cGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAg                                                                                                                                   >  8:10892/1‑149 (MQ=255)                         aGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGAt                                                                                                                              <  5:165567/149‑1 (MQ=255)                           gCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATc                                                                                                                             >  8:56020/1‑148 (MQ=255)                                    aGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGa                                                                                                                    <  2:56733/148‑1 (MQ=255)                                        tgctgcCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGATCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCtt                                                                                                              >  3:167478/1‑149 (MQ=255)                                         gctgcCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCtt                                                                                                              >  2:102791/1‑148 (MQ=255)                                          ctgcCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCa                                                                                                            <  2:154857/149‑1 (MQ=255)                                           tgcCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAg                                                                                                           >  4:338455/1‑149 (MQ=255)                                             ccAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAgg                                                                                                          <  3:264974/148‑1 (MQ=255)                                              cAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCt                                                                                                        >  2:199064/1‑149 (MQ=255)                                              cAGCGTACCAGAGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCt                                                                                                        >  8:114530/1‑149 (MQ=255)                                                 cGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTc                                                                                                     <  2:304965/149‑1 (MQ=255)                                                  gTACCAGCGCTTCCAGTTTGTCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCa                                                                                                    <  4:44168/149‑1 (MQ=255)                                                   tACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCAt                                                                                                   <  2:276331/149‑1 (MQ=255)                                                      cAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTg                                                                                                 >  4:368505/1‑148 (MQ=255)                                                      cAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAACATCGGCAGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGAGCCAGGCGCAGCTCTTCTTCCAATTGCAAGCCGATCGACGCCCGAG‑CCTACACGCTGTCAttt                                                                                                 >  7:142865/1‑147 (MQ=255)                                                      cAGCGCTTCCAGTATCGCGACTTGCCCGCTGCGCAGGTCGAAGCTCGGCTGGTAGAGCACACACAGCGCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTGCAGTTGCAAGGCCATCGACGCGCGAG‑CCTTGAGGCTCTCAtag                                                                                                 >  6:33462/1‑146 (MQ=255)                                                          gcTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTgaagaa                                                                                            <  7:10892/149‑1 (MQ=255)                                                            ttCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAAt                                                                                          >  5:250272/1‑149 (MQ=255)                                                                aGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAg                                                                                      >  8:292472/1‑149 (MQ=255)                                                                  tttGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGc                                                                                    <  3:317551/149‑1 (MQ=255)                                                                  tttGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGc                                                                                    >  4:199365/1‑149 (MQ=255)                                                                      gCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGtt                                                                                <  3:199365/149‑1 (MQ=255)                                                                       ccACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCAt                                                                                                   >  2:128714/1‑129 (MQ=255)                                                                       ccACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCAt                                                                                                   <  1:128714/129‑1 (MQ=255)                                                                       ccACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTg                                                                               >  3:303186/1‑149 (MQ=255)                                                                        cACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGtt                                                                                >  3:49888/1‑147 (MQ=255)                                                                           ttGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCt                                                                                                        <  4:6524/120‑1 (MQ=255)                                                                           ttGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCt                                                                                                        >  3:6524/1‑120 (MQ=255)                                                                              cccGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGt                                                                        >  1:168601/1‑149 (MQ=255)                                                                                cGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTg                                                                      >  6:234053/1‑149 (MQ=255)                                                                                 gCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTAGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATAGAAGAAATTCAGGCTGTTGCGCCCGTTGt                                                                     >  6:196923/1‑149 (MQ=255)                                                                                      gcAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTg                                                                <  3:102152/149‑1 (MQ=255)                                                                                      gcAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTg                                                                <  7:292472/149‑1 (MQ=255)                                                                                      gcAGATCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTg                                                                <  4:167478/149‑1 (MQ=255)                                                                                       cAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTgg                                                               >  7:210236/1‑149 (MQ=255)                                                                                       cAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTgg                                                               <  8:149675/149‑1 (MQ=255)                                                                                        aGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGa                                                              >  4:17873/1‑149 (MQ=255)                                                                                          gTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGAtt                                                            >  3:305727/1‑149 (MQ=255)                                                                                           tCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTg                                                           <  6:250272/149‑1 (MQ=255)                                                                                            cGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTgg                                                          >  3:344379/1‑149 (MQ=255)                                                                                             gAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTgg                                                          <  1:102791/149‑1 (MQ=255)                                                                                             gAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTgg                                                          >  4:187916/1‑148 (MQ=255)                                                                                             gAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGt                                                         <  3:204773/149‑1 (MQ=255)                                                                                             gAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGt                                                         <  4:303186/149‑1 (MQ=255)                                                                                                 aTCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTagag                                                     >  2:362882/1‑149 (MQ=255)                                                                                                  tCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAgg                                                    <  3:17873/149‑1 (MQ=255)                                                                                                     gCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGcc                                                 <  7:114530/149‑1 (MQ=255)                                                                                                       tGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAg                                               <  8:193600/149‑1 (MQ=255)                                                                                                        ggTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAgg                                              >  6:33513/1‑149 (MQ=255)                                                                                                         gTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAgg                                                    >  1:281764/1‑142 (MQ=255)                                                                                                         gTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAgg                                                    <  2:281764/142‑1 (MQ=255)                                                                                                         gTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGt                                             >  5:369046/1‑149 (MQ=255)                                                                                                            gTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCgg                                          <  4:344379/149‑1 (MQ=255)                                                                                                               cacacacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCAt                                       >  7:300340/1‑149 (MQ=255)                                                                                                               cacacacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCAt                                       >  3:251353/1‑149 (MQ=255)                                                                                                                  acacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCt                                    >  7:336804/1‑149 (MQ=255)                                                                                                                  acacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCt                                    >  8:259158/1‑149 (MQ=255)                                                                                                                  acacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCt                                    >  2:55288/1‑149 (MQ=255)                                                                                                                  acacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGGTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCt                                    >  7:312947/1‑149 (MQ=255)                                                                                                                  acacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGACATTGAAGAAATTCAGGCTGTTGCGCCAGTTGTCCTTGGATTGGTAGAGGGcc                                                 >  8:184946/1‑136 (MQ=255)                                                                                                                    acaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCtt                                   >  5:151802/1‑148 (MQ=255)                                                                                                                      aGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGag                                <  8:336804/149‑1 (MQ=255)                                                                                                                       gCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGagc                               <  5:196923/149‑1 (MQ=255)                                                                                                                        cccGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGag                                >  2:292374/1‑147 (MQ=255)                                                                                                                          cgcgCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCGTTGGGTTGGTAGAGGGCCAGGTCGGCATGCTTGagcagc                            >  5:24274/1‑149 (MQ=255)                                                                                                                          cgcgCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATACAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGa                                 >  8:46170/1‑144 (MQ=255)                                                                                                                          cgcgCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATACAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGa                                 <  7:46170/144‑1 (MQ=255)                                                                                                                              ctctTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCtt                        <  5:153739/149‑1 (MQ=255)                                                                                                                                 ttCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTc                       <  6:41292/147‑1 (MQ=255)                                                                                                                                 ttCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCgg                     <  4:49888/149‑1 (MQ=255)                                                                                                                                   cGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCAGCATGCTTGAGCAGCTCTTCgg                     <  7:56020/147‑1 (MQ=255)                                                                                                                                    gAGCTGCGCCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCtg                  >  2:127715/1‑149 (MQ=255)                                                                                                                                    gAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCt                   <  1:199064/148‑1 (MQ=255)                                                                                                                                       ctgcgcCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGagc                               <  7:156269/131‑1 (MQ=255)                                                                                                                                       ctgcgcCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGagc                               >  8:156269/3‑133 (MQ=255)                                                                                                                                        agcGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGtgct              >  4:193149/1‑149 (MQ=255)                                                                                                                                         gcgcCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGtgctg             <  5:33513/149‑1 (MQ=255)                                                                                                                                              aGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGTGCTGCCATc        <  8:210236/149‑1 (MQ=255)                                                                                                                                                gcgcAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGAATGCTTGAGCAGCTCTTCGGCTGTGCTGCCATCGt      >  8:240585/1‑149 (MQ=255)                                                                                                                                                  gcAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGTGCTGCCATCGTcc    >  3:198730/1‑149 (MQ=255)                                                                                                                                                   cAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCTGCTGTGCTGCCATCGTCCg   >  7:179555/1‑149 (MQ=255)                                                                                                                                                    aGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGTGCTGCCATCGTCCgg  <  3:368505/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      CCGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAG‑CCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGTGCTGCCATCGTCCGG  >  NC_002947/1309983‑1310278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑