Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,040 G→C 8.2% intergenic (+307/‑85) PP_1180 → / → PP_1181 hypothetical protein/two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,0400GC 8.2% 186.0 / 6.9 61intergenic (+307/‑85)PP_1180/PP_1181hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base C (2/3);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCG  >  NC_002947/1355895‑1356179
                                                                                                                                                 |                                                                                                                                             
gTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGgc                                                                                                                                            <  8:323862/149‑1 (MQ=255)
    tttGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGTGCACCGCCCGCGGGGCGCTc                                                                                                                                        <  6:384858/149‑1 (MQ=255)
      tGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGTCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCgg                                                                                                                                      >  2:299183/1‑149 (MQ=255)
              ttttCTGCACCGTGACGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTGCAGTCGAGGGGGCGGTAGGGCGTGGCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCAg                                                                                                                              <  5:377197/149‑1 (MQ=25)
              ttttCAGCACCGTGATGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGTGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCa                                                                                                                               <  8:231527/148‑1 (MQ=25)
                 tCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                              <  5:345643/130‑1 (MQ=255)
                 tCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                              >  6:345643/1‑130 (MQ=255)
                 tCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCGGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAACATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAggg                                                                                                                            <  4:344125/148‑1 (MQ=255)
                     cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGcccc                                                                                                                        <  4:116068/148‑1 (MQ=255)
                      aCCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccccc                                                                                                                       >  3:177538/1‑148 (MQ=255)
                        cGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑t                                                                                                                   <  5:362375/149‑1 (MQ=255)
                         gTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑Tc                                                                                                                  >  8:242742/1‑149 (MQ=255)
                          tGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑Tc                                                                                                                  >  1:37475/1‑148 (MQ=255)
                          tGAAGCCCTTGGGCAGTTTATTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCt                                                                                                                 <  1:124080/149‑1 (MQ=255)
                            aaGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTa                                                                                                                <  7:242742/148‑1 (MQ=255)
                                ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtc                                                                                                                                    >  4:63316/1‑125 (MQ=37)
                                ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtc                                                                                                                                    <  3:63316/125‑1 (MQ=37)
                                 cTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCa                                                                                                          >  7:80361/1‑149 (MQ=255)
                                 cTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCa                                                                                                          >  3:251926/1‑149 (MQ=255)
                                  ttGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCa                                                                                                          >  3:239972/1‑148 (MQ=255)
                                       cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                                         <  1:347445/113‑1 (MQ=255)
                                       cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                                         >  2:347445/1‑113 (MQ=255)
                                       cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGGCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TActct                                                                                                   <  2:279773/149‑1 (MQ=255)
                                        aGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACtctc                                                                                                  <  6:136018/149‑1 (MQ=255)
                                         gTTTTTTTTTGGCGCGAAGCCTGTCACTAAAAACGGTTCAGTCTAGGGGGCGGTAGGGCTTGTCTGAAGATCTGGTGTGCCTGGGATATCGAGCACCGCCCTCGGGGCGCTCGGTCTAGCAGGGCCCCCCA‑TCTACATTAA‑TACTCTCa                                                                                                 <  5:212591/149‑1 (MQ=255)
                                          ttttttttGGGCGCGAAGCCTTTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                                         <  4:24997/110‑1 (MQ=255)
                                          ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                                         >  3:24997/1‑110 (MQ=255)
                                                   ggCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGa                                                                                       <  8:87840/149‑1 (MQ=255)
                                                           gCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATcc                                                                               <  2:37475/149‑1 (MQ=255)
                                                            ccTGTCAGAAATAAGGGTTCTGTCGAGGGGGCGGTAGGGCGTGTCTGATGATCTGGGGTGCCTGTGATATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCT‑TACTCTCAGCTGCCCTGACACGATccc                                                                              <  2:250264/149‑1 (MQ=255)
                                                             cTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATccc                                                                              >  4:245758/1‑148 (MQ=255)
                                                               gTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCaa                                                                            >  7:328565/1‑148 (MQ=255)
                                                                  acaAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGTTGCCTGTTATATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGaa                                                                         <  1:299183/148‑1 (MQ=255)
                                                                      aaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACtctc                                                                                                  <  6:346115/119‑1 (MQ=255)
                                                                      aaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACtctc                                                                                                  >  5:346115/1‑119 (MQ=255)
                                                                        aaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGGCTCGCAGGGCCCCCCACTCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAAcaacaa                                                                   <  1:274341/149‑1 (MQ=255)
                                                                         aCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAgg                                                                 <  4:251926/149‑1 (MQ=255)
                                                                                    gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                      <  8:98378/149‑1 (MQ=255)
                                                                                    gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                      >  8:231204/1‑149 (MQ=255)
                                                                                    gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                      <  2:36958/149‑1 (MQ=255)
                                                                                            ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                              >  2:358966/1‑149 (MQ=255)
                                                                                                 ggCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGac                                                   >  3:182290/1‑139 (MQ=255)
                                                                                                 ggCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGac                                                   <  4:182290/139‑1 (MQ=255)
                                                                                                       tCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGc                                    <  4:239972/148‑1 (MQ=255)
                                                                                                         tGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                 >  6:57451/1‑149 (MQ=255)
                                                                                                         tGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                 >  7:27796/1‑149 (MQ=255)
                                                                                                          gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCAGTACTCTCAGCTGCCCTGACACGATCCCAAGa                                                                          <  1:39486/108‑1 (MQ=255)
                                                                                                          gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGaacaac                                                                     <  5:246860/112‑1 (MQ=255)
                                                                                                          gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGaacaac                                                                     >  6:246860/1‑112 (MQ=255)
                                                                                                          gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                      <  6:339690/127‑1 (MQ=255)
                                                                                                          gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATcc                                >  5:302744/1‑149 (MQ=255)
                                                                                                          gAAGATCTGGGGTGCCTCGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACAACCCCATACCCCGCATc                                 >  4:149791/1‑148 (MQ=255)
                                                                                                           aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCt                               >  7:115443/1‑149 (MQ=255)
                                                                                                            aGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCt                               >  6:19839/1‑148 (MQ=255)
                                                                                                             gATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTc                              >  8:31017/1‑148 (MQ=255)
                                                                                                               tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCCTCCTCAtc                           >  4:175962/1‑149 (MQ=255)
                                                                                                                    gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtcg                       >  6:312170/5‑147 (MQ=21)
                                                                                                                    ggTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGa                      <  1:358966/149‑1 (MQ=255)
                                                                                                                          tGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacga                >  2:314589/1‑149 (MQ=255)
                                                                                                                            ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac               <  7:31017/148‑1 (MQ=255)
                                                                                                                                   gAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCt       <  8:115443/149‑1 (MQ=255)
                                                                                                                                    aGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTc      <  3:149791/149‑1 (MQ=255)
                                                                                                                                         cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCGACATTCA‑TACTCTCCGCTGCCCTGACACGATCCCAAGACCAACAAGGGCACAGCCATGGACCCCCCCATACCCCGCCTCCTCACCGTCGAAGACGACCAGCGCCTCGCCg  >  7:270901/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCG  >  NC_002947/1355895‑1356179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑