Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,049 G→A 8.5% intergenic (+316/‑76) PP_1180 → / → PP_1181 hypothetical protein/two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,0490GA 8.5% 170.7 / 5.1 59intergenic (+316/‑76)PP_1180/PP_1181hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base A (2/3);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

TGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGAA  >  NC_002947/1355901‑1356193
                                                                                                                                                    |                                                                                                                                                  
tGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGTCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCgg                                                                                                                                                    >  2:299183/1‑149 (MQ=255)
        ttttCTGCACCGTGACGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTGCAGTCGAGGGGGCGGTAGGGCGTGGCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCAg                                                                                                                                            <  5:377197/149‑1 (MQ=25)
        ttttCAGCACCGTGATGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGTGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCa                                                                                                                                             <  8:231527/148‑1 (MQ=25)
           tCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCGGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAACATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAggg                                                                                                                                          <  4:344125/148‑1 (MQ=255)
               cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGcccc                                                                                                                                      <  4:116068/148‑1 (MQ=255)
                aCCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccccc                                                                                                                                     >  3:177538/1‑148 (MQ=255)
                  cGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑t                                                                                                                                 <  5:362375/149‑1 (MQ=255)
                   gTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑Tc                                                                                                                                >  8:242742/1‑149 (MQ=255)
                    tGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑Tc                                                                                                                                >  1:37475/1‑148 (MQ=255)
                    tGAAGCCCTTGGGCAGTTTATTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCt                                                                                                                               <  1:124080/149‑1 (MQ=255)
                      aaGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTa                                                                                                                              <  7:242742/148‑1 (MQ=255)
                          ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtc                                                                                                                                                  >  4:63316/1‑125 (MQ=37)
                          ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtc                                                                                                                                                  <  3:63316/125‑1 (MQ=37)
                           cTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCa                                                                                                                        >  7:80361/1‑149 (MQ=255)
                           cTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCa                                                                                                                        >  3:251926/1‑149 (MQ=255)
                            ttGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCa                                                                                                                        >  3:239972/1‑148 (MQ=255)
                                 cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGGCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TActct                                                                                                                 <  2:279773/149‑1 (MQ=255)
                                  aGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACtctc                                                                                                                <  6:136018/149‑1 (MQ=255)
                                   gTTTTTTTTTGGCGCGAAGCCTGTCACTAAAAACGGTTCAGTCTAGGGGGCGGTAGGGCTTGTCTGAAGATCTGGTGTGCCTGGGATATCGAGCACCGCCCTCGGGGCGCTCGGTCTAGCAGGGCCCCCCA‑TCTACATTAA‑TACTCTCa                                                                                                               <  5:212591/149‑1 (MQ=255)
                                             ggCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGa                                                                                                     <  8:87840/149‑1 (MQ=255)
                                                     gCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATcc                                                                                             <  2:37475/149‑1 (MQ=255)
                                                      ccTGTCAGAAATAAGGGTTCTGTCGAGGGGGCGGTAGGGCGTGTCTGATGATCTGGGGTGCCTGTGATATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCT‑TACTCTCAGCTGCCCTGACACGATccc                                                                                            <  2:250264/149‑1 (MQ=255)
                                                       cTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATccc                                                                                            >  4:245758/1‑148 (MQ=255)
                                                         gTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCaa                                                                                          >  7:328565/1‑148 (MQ=255)
                                                            acaAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGTTGCCTGTTATATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGaa                                                                                       <  1:299183/148‑1 (MQ=255)
                                                                aaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACtctc                                                                                                                <  6:346115/119‑1 (MQ=255)
                                                                aaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACtctc                                                                                                                >  5:346115/1‑119 (MQ=255)
                                                                  aaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGGCTCGCAGGGCCCCCCACTCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAAcaacaa                                                                                 <  1:274341/149‑1 (MQ=255)
                                                                   aCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAgg                                                                               <  4:251926/149‑1 (MQ=255)
                                                                              gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                                    <  8:98378/149‑1 (MQ=255)
                                                                              gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                                    >  8:231204/1‑149 (MQ=255)
                                                                              gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                                    <  2:36958/149‑1 (MQ=255)
                                                                                      ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                                            >  2:358966/1‑149 (MQ=255)
                                                                                           ggCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGac                                                                 >  3:182290/1‑139 (MQ=255)
                                                                                           ggCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGac                                                                 <  4:182290/139‑1 (MQ=255)
                                                                                                 tCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGc                                                  <  4:239972/148‑1 (MQ=255)
                                                                                                   tGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                               >  7:27796/1‑149 (MQ=255)
                                                                                                   tGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                               >  6:57451/1‑149 (MQ=255)
                                                                                                    gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCAGTACTCTCAGCTGCCCTGACACGATCCCAAGa                                                                                        <  1:39486/108‑1 (MQ=255)
                                                                                                    gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGaacaac                                                                                   <  5:246860/112‑1 (MQ=255)
                                                                                                    gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGaacaac                                                                                   >  6:246860/1‑112 (MQ=255)
                                                                                                    gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATg                                                                    <  6:339690/127‑1 (MQ=255)
                                                                                                    gAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATcc                                              >  5:302744/1‑149 (MQ=255)
                                                                                                    gAAGATCTGGGGTGCCTCGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACAACCCCATACCCCGCATc                                               >  4:149791/1‑148 (MQ=255)
                                                                                                     aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCt                                             >  7:115443/1‑149 (MQ=255)
                                                                                                      aGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCt                                             >  6:19839/1‑148 (MQ=255)
                                                                                                       gATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTc                                            >  8:31017/1‑148 (MQ=255)
                                                                                                         tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCCTCCTCAtc                                         >  4:175962/1‑149 (MQ=255)
                                                                                                              gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtcg                                     >  6:312170/5‑147 (MQ=21)
                                                                                                              ggTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGa                                    <  1:358966/149‑1 (MQ=255)
                                                                                                                    tGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacga                              >  2:314589/1‑149 (MQ=255)
                                                                                                                      ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac                             <  7:31017/148‑1 (MQ=255)
                                                                                                                             gAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCt                     <  8:115443/149‑1 (MQ=255)
                                                                                                                              aGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTc                    <  3:149791/149‑1 (MQ=255)
                                                                                                                                   cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCGACATTCA‑TACTCTCCGCTGCCCTGACACGATCCCAAGACCAACAAGGGCACAGCCATGGACCCCCCCATACCCCGCCTCCTCACCGTCGAAGACGACCAGCGCCTCGCCg                >  7:270901/1‑148 (MQ=255)
                                                                                                                                             gcgcTCGGTCTCGCAGGGCCCGCCA‑TCTACATTCA‑TACTCTCAGCAGCCCTGACACGAGACCAAGAACAACAAGGCCAACGCCATGGACCAGCCCAAACACCGCAGCCTCAACGGCGAAGACGACCACCGCCTCGCCGCACTCAccgcc     >  4:41268/1‑149 (MQ=255)
                                                                                                                                               gcTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGa   <  8:80361/149‑1 (MQ=255)
                                                                                                                                                cTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGaa  <  3:245758/149‑1 (MQ=255)
                                                                                                                                                cTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGaa  <  5:19839/149‑1 (MQ=255)
                                                                                                                                                 tCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGaa  >  7:269913/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCA‑TCTACATTCA‑TACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGAA  >  NC_002947/1355901‑1356193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑