Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 155,546 A→C 8.3% intergenic (‑251/+32) yjbB ← / ← ygdQ Na+/Pi‑cotransporter/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947155,5460AC 8.3% 210.2 / 9.1 72intergenic (‑251/+32)yjbB/ygdQNa+/Pi‑cotransporter/transport protein
Reads supporting (aligned to +/- strand):  ref base A (34/32);  new base C (2/4);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

TCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGGCCA  >  NC_002947/155401‑155693
                                                                                                                                                 |                                                                                                                                                   
tCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAgg                                                                                                                                                  >  6:12236/1‑149 (MQ=255)
  gCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGCTCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAggg                                                                                                                                                 <  8:337058/148‑1 (MQ=255)
  gCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGc                                                                                                                                                <  3:365527/149‑1 (MQ=255)
  gCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAggg                                                                                                                                                 <  6:9893/148‑1 (MQ=255)
  gCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGc                                                                                                                                                <  8:77822/149‑1 (MQ=255)
       cTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGTGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCttt                                                                                                                                           >  5:360922/1‑148 (MQ=255)
       cTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttg                                                                                                                                           <  3:90627/149‑1 (MQ=255)
          cGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGTGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCttttt                                                                                                                                         <  6:360922/148‑1 (MQ=255)
          cGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCttttt                                                                                                                                         <  5:68712/148‑1 (MQ=255)
          cGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttgtt                                                                                                                                         <  3:39498/148‑1 (MQ=255)
          cGCATGCCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGTGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGTCATTAAAAAGGGCCCAGCGAAGGGCCAttgttg                                                                                                                                        >  8:270832/1‑149 (MQ=255)
           gCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCtttttaa                                                                                                                                       <  8:121685/149‑3 (MQ=255)
            cATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAggg                                                                                                                                                 <  5:98870/138‑1 (MQ=255)
            cATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAggg                                                                                                                                                 >  6:98870/1‑138 (MQ=255)
             aTGGCAGTCATTTATTTGAAACATATCTTGAAACGAATGACTTCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgc                                                                                                                                     <  7:270832/149‑1 (MQ=255)
              tGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgct                                                                                                                                    <  6:374542/149‑1 (MQ=255)
                gCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgctgc                                                                                                                                  <  2:85747/149‑1 (MQ=255)
                gCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgctg                                                                                                                                   <  3:289864/148‑1 (MQ=255)
                  aGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCAc                                                                                                                                <  4:265839/149‑1 (MQ=255)
                          atttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTgg                                                                                                                        >  5:210592/1‑149 (MQ=255)
                              gAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCt                                                                                                                     >  6:346325/1‑148 (MQ=255)
                               aaaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAACCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTGGTGCTGCACGTCCTTGGCATTa                                                                                                                   >  6:273894/1‑149 (MQ=255)
                                   aGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGa                                                                                                               >  7:213896/1‑149 (MQ=255)
                                    gATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGAc                                                                                                              >  3:52729/1‑149 (MQ=255)
                                        ttGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGCCAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCg                                                                                                           >  2:162192/1‑148 (MQ=255)
                                        ttGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCg                                                                                                           >  7:147617/1‑148 (MQ=255)
                                          gAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGAccc                                                                                                            <  3:301837/145‑1 (MQ=255)
                                            aaCGAATGACTGCCCGTGTGGGCTTCTTCGCGGGCAAGCAACCACAGTTACAACGCGCATGAAAAAACTCACAGAAAAAAGGCATTAAAAAGGTCCATGCGAAGTGCCCTTGTTGTTCTGCACTTCCTTGGCCTTATTTACCCGGAAt                                                                                                       <  8:172209/148‑1 (MQ=255)
                                            aaCGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATg                                                                                                      >  8:54441/1‑149 (MQ=255)
                                              cGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGAGCCGGAATGTc                                                                                                    >  4:121552/1‑149 (MQ=255)
                                              cGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTc                                                                                                    >  6:249608/1‑149 (MQ=255)
                                              cGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTc                                                                                                    <  5:346325/149‑1 (MQ=255)
                                               gAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTcc                                                                                                   >  7:177052/1‑149 (MQ=255)
                                                 aTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCtt                                                                                                 <  7:54441/149‑1 (MQ=255)
                                                 aTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCtt                                                                                                 <  8:155438/149‑1 (MQ=255)
                                                     cTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTgcgc                                                                                             <  1:5275/149‑1 (MQ=255)
                                                     cTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTgcg                                                                                              >  1:169651/1‑148 (MQ=255)
                                                      tGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAtt                                                                                                                 <  8:97550/128‑1 (MQ=255)
                                                       gCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAg                                                                                           >  5:90165/1‑149 (MQ=255)
                                                        cccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGt                                                                                          >  1:100999/1‑149 (MQ=255)
                                                         ccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGtt                                                                                         >  7:241557/1‑149 (MQ=255)
                                                                  ctgctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGtt                                                                                <  8:229805/149‑1 (MQ=255)
                                                                     ctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGt                                                                                          >  1:12962/1‑136 (MQ=255)
                                                                     ctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGt                                                                                          <  2:12962/136‑1 (MQ=255)
                                                                            ggCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCtt                                                                      <  5:12236/149‑1 (MQ=255)
                                                                                 gCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTg                                                                     >  6:142807/1‑145 (MQ=255)
                                                                                 gCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTg                                                                     <  5:142807/145‑1 (MQ=255)
                                                                                  ccaccaCAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCAt                                                                 >  1:376232/1‑148 (MQ=255)
                                                                                    accacAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCt                                                              >  5:172741/1‑149 (MQ=255)
                                                                                         aGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGTCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                         <  8:241557/149‑1 (MQ=255)
                                                                                          gTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCggg                                                        >  8:119606/1‑149 (MQ=255)
                                                                                               aaCGCGCAGGAAAAAACTCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTTCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATg                                                   <  2:17452/149‑1 (MQ=255)
                                                                                                aCGCGCAGGAAAAACCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATgg                                                  <  3:227469/149‑1 (MQ=255)
                                                                                                 cgcgCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGc                                                 >  5:258004/1‑149 (MQ=255)
                                                                                                    gcAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGt                                              <  1:162192/149‑1 (MQ=255)
                                                                                                          aaaaaCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCAt                                        >  1:222013/1‑149 (MQ=255)
                                                                                                                gCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGAt                                  <  2:100999/149‑1 (MQ=255)
                                                                                                                  acaGAAAAAAGGCATTAAAATGGGCCCTGCGAAGGGCCCTTGTTGTTCTGCACTTCCTTGGCCTTATTGACCCGGACTGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGt                                <  7:139861/149‑1 (MQ=255)
                                                                                                                    aGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                         >  3:260195/1‑122 (MQ=255)
                                                                                                                     gAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGa                             >  1:309303/1‑149 (MQ=255)
                                                                                                                      aaaaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                            <  6:18355/149‑1 (MQ=255)
                                                                                                                        aaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATgg                          >  2:221127/1‑149 (MQ=255)
                                                                                                                        aaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATgg                          >  7:325575/1‑149 (MQ=255)
                                                                                                                          aaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCt                        <  4:52729/149‑1 (MQ=255)
                                                                                                                             gCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTc                      >  6:254094/1‑148 (MQ=255)
                                                                                                                               aTTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCAc                   >  1:309046/1‑149 (MQ=255)
                                                                                                                                ttAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCAcc                  >  1:369168/1‑149 (MQ=255)
                                                                                                                                   aaaaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGcc               <  8:147617/149‑1 (MQ=255)
                                                                                                                                    caaGGGCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGc                >  6:46025/2‑147 (MQ=255)
                                                                                                                                         gCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGc            >  4:110768/1‑146 (MQ=255)
                                                                                                                                          cccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCgag         >  5:294728/1‑148 (MQ=255)
                                                                                                                                                gAACGGCCCTTGTTGTGCTGCACTTCCTTGGCCGAATTTACCCGGAATGTCCTTGCGCAGGTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGGCCa  <  4:228503/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGGCCA  >  NC_002947/155401‑155693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑