Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,381,492 C→A 6.4% Y81* (TAC→TAA) ‡ dinB → DNA polymerase IV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,381,4920CA 6.4% 233.2 / 2.6 72Y81* (TAC→TAA) ‡dinBDNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base C (34/33);  new base A (3/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGGCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATT  >  NC_002947/1381349‑1381633
                                                                                                                                               |                                                                                                                                               
gggggCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGt                                                                                                                                              >  4:109031/1‑147 (MQ=255)
gggggCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGt                                                                                                                                              >  6:166439/1‑147 (MQ=255)
 ggggCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAg                                                                                                                                           <  3:367256/149‑1 (MQ=255)
    gCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGcc                                                                                                                                        <  2:360128/149‑1 (MQ=255)
     cTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTCACGGTAGGCCt                                                                                                                                       >  5:114592/1‑149 (MQ=255)
      tCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCACGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTc                                                                                                                                      >  2:109738/1‑149 (MQ=255)
          ccGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTCACGGTAGGCCTcgaag                                                                                                                                  <  6:114592/149‑4 (MQ=255)
          ccGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCgg                                                                                                                                  >  2:314672/1‑149 (MQ=255)
          ccGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCgg                                                                                                                                  >  2:65207/1‑149 (MQ=255)
           cGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTCACGGTAGGCCTcgaagc                                                                                                                                 <  1:327421/149‑5 (MQ=255)
              ccATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGaaa                                                                                                                              >  5:261927/1‑149 (MQ=255)
              ccATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGaaa                                                                                                                              >  8:203809/1‑149 (MQ=255)
                   gcgGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCcac                                                                                                                         <  3:207533/149‑1 (MQ=255)
                         tAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATc                                                                                                                   <  8:273359/149‑1 (MQ=255)
                            tCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGAGCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCttt                                                                                                                >  6:314970/1‑149 (MQ=255)
                             cGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTc                                                                                                               >  8:201441/1‑149 (MQ=255)
                              gCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTcg                                                                                                              <  5:200774/149‑1 (MQ=255)
                              gCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTc                                                                                                               <  5:341127/148‑1 (MQ=255)
                                    tGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTa                                                                                                        >  6:384257/1‑149 (MQ=255)
                                         cTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACg                                                                                                    >  8:232449/1‑148 (MQ=255)
                                          tATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACg                                                                                                    >  4:72722/1‑147 (MQ=255)
                                          tATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACg                                                                                                    >  4:168331/1‑147 (MQ=255)
                                            tGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGc                                                                                                >  5:165743/1‑149 (MQ=255)
                                                 cgcgTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATc                                                                                           <  7:203809/149‑1 (MQ=255)
                                                    gTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAg                                                                                        >  8:266743/1‑149 (MQ=255)
                                                        cTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCtt                                                                                    >  1:69090/1‑149 (MQ=255)
                                                         tATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                   >  7:79416/1‑149 (MQ=255)
                                                         tATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                   >  5:217732/1‑149 (MQ=255)
                                                          aTGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTg                                                                                  >  7:261097/1‑149 (MQ=255)
                                                           tGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                   <  1:65207/147‑1 (MQ=255)
                                                                tgCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCt                                                                             <  6:217732/148‑1 (MQ=255)
                                                                  cgcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGa                                                                          >  6:319599/1‑149 (MQ=255)
                                                                  cgcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGAGCTGTTGATCGTCAAGCCGCTCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGa                                                                          <  3:59418/149‑1 (MQ=255)
                                                                   gcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCt                                                                                                                  <  4:125584/108‑1 (MQ=255)
                                                                   gcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCt                                                                                                                  >  3:125584/1‑108 (MQ=255)
                                                                       ggCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAg                                                                   <  1:109738/149‑1 (MQ=255)
                                                                       ggCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAg                                                                   <  7:327464/149‑1 (MQ=255)
                                                                          cATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCtt                                                                <  3:168331/149‑1 (MQ=255)
                                                                                aTCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTg                                                           >  5:33036/1‑148 (MQ=255)
                                                                                aTCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTg                                                           <  3:153382/148‑1 (MQ=255)
                                                                                aTCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTg                                                           >  7:41759/1‑148 (MQ=255)
                                                                                 tCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGa                                                         <  5:296307/149‑1 (MQ=255)
                                                                                 tCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGa                                                         >  4:63198/1‑149 (MQ=255)
                                                                                  cgcgGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGAt                                                        <  4:359464/149‑1 (MQ=255)
                                                                                   gcgGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGAt                                                        <  3:109031/148‑1 (MQ=255)
                                                                                        aTGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGc                                                  <  4:77723/149‑1 (MQ=255)
                                                                                         tGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGGAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGc                                                  >  4:10460/1‑148 (MQ=255)
                                                                                            gcTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACa                                              <  4:316582/149‑1 (MQ=255)
                                                                                            gcTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACa                                              <  1:314672/149‑1 (MQ=255)
                                                                                                aaGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGcc                                           <  7:266743/148‑1 (MQ=255)
                                                                                                aaGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGc                                            >  3:200031/1‑147 (MQ=255)
                                                                                                   cTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTg                                       <  3:72722/149‑1 (MQ=255)
                                                                                                   cTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTg                                       <  6:261927/149‑1 (MQ=255)
                                                                                                    tttGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                   <  5:344967/106‑1 (MQ=255)
                                                                                                    tttGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                   >  6:344967/1‑106 (MQ=255)
                                                                                                       gCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTa                                    <  8:79416/148‑1 (MQ=255)
                                                                                                          ccGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCCCACGATCTTTCGCGATTCCACGCAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCg                                <  6:165743/149‑1 (MQ=255)
                                                                                                             aTCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCggg                              >  1:348804/1‑148 (MQ=255)
                                                                                                                 gTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAgcg                         >  2:191298/1‑149 (MQ=255)
                                                                                                                 gTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGAACCTTGAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAg                           >  1:126017/1‑149 (MQ=255)
                                                                                                                     aTCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCAc                     <  3:63198/149‑1 (MQ=255)
                                                                                                                            aGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCAtt              <  5:384257/149‑1 (MQ=255)
                                                                                                                             gCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCAGCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTg             <  5:319599/149‑1 (MQ=255)
                                                                                                                               cgcgCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGcc           >  1:62799/1‑149 (MQ=255)
                                                                                                                                 cgcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGa         <  5:166439/149‑1 (MQ=255)
                                                                                                                                  gcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg        <  5:314970/149‑1 (MQ=255)
                                                                                                                                   ccgcGAGGCCTCACGGTAGGCCTCGCGGGAAATCCCCACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg        >  6:268517/4‑148 (MQ=255)
                                                                                                                                   ccgcGAGGCCTCACGGTAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg        >  2:20382/4‑148 (MQ=255)
                                                                                                                                   cTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg        >  8:355035/1‑148 (MQ=255)
                                                                                                                                        aGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCgg                               >  4:243199/1‑120 (MQ=255)
                                                                                                                                        aGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCgg                               <  3:243199/120‑1 (MQ=255)
                                                                                                                                        aGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATAtt  >  1:166669/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GGGGGCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATT  >  NC_002947/1381349‑1381633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑