Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,381,496 G→T 6.8% E83* (GAG→TAG)  dinB → DNA polymerase IV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,381,4960GT 6.8% 230.2 / 3.8 71E83* (GAG→TAG) dinBDNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base G (32/34);  new base T (3/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCG  >  NC_002947/1381350‑1381641
                                                                                                                                                  |                                                                                                                                                   
ggggCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAg                                                                                                                                                   <  3:367256/149‑1 (MQ=255)
   gCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGcc                                                                                                                                                <  2:360128/149‑1 (MQ=255)
    cTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTCACGGTAGGCCt                                                                                                                                               >  5:114592/1‑149 (MQ=255)
     tCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCACGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTc                                                                                                                                              >  2:109738/1‑149 (MQ=255)
         ccGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTCACGGTAGGCCTcgaag                                                                                                                                          <  6:114592/149‑4 (MQ=255)
         ccGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCgg                                                                                                                                          >  2:65207/1‑149 (MQ=255)
         ccGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCgg                                                                                                                                          >  2:314672/1‑149 (MQ=255)
          cGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTCACGGTAGGCCTcgaagc                                                                                                                                         <  1:327421/149‑5 (MQ=255)
             ccATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGaaa                                                                                                                                      >  5:261927/1‑149 (MQ=255)
             ccATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGaaa                                                                                                                                      >  8:203809/1‑149 (MQ=255)
                  gcgGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCcac                                                                                                                                 <  3:207533/149‑1 (MQ=255)
                        tAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATc                                                                                                                           <  8:273359/149‑1 (MQ=255)
                           tCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGAGCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCttt                                                                                                                        >  6:314970/1‑149 (MQ=255)
                            cGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTc                                                                                                                       >  8:201441/1‑149 (MQ=255)
                             gCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTcg                                                                                                                      <  5:200774/149‑1 (MQ=255)
                             gCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTc                                                                                                                       <  5:341127/148‑1 (MQ=255)
                                   tGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTa                                                                                                                >  6:384257/1‑149 (MQ=255)
                                        cTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACg                                                                                                            >  8:232449/1‑148 (MQ=255)
                                         tATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACg                                                                                                            >  4:168331/1‑147 (MQ=255)
                                         tATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACg                                                                                                            >  4:72722/1‑147 (MQ=255)
                                           tGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGc                                                                                                        >  5:165743/1‑149 (MQ=255)
                                                cgcgTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATc                                                                                                   <  7:203809/149‑1 (MQ=255)
                                                   gTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAg                                                                                                >  8:266743/1‑149 (MQ=255)
                                                       cTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCtt                                                                                            >  1:69090/1‑149 (MQ=255)
                                                        tATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                           >  7:79416/1‑149 (MQ=255)
                                                        tATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                           >  5:217732/1‑149 (MQ=255)
                                                         aTGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTg                                                                                          >  7:261097/1‑149 (MQ=255)
                                                          tGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                           <  1:65207/147‑1 (MQ=255)
                                                               tgCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCt                                                                                     <  6:217732/148‑1 (MQ=255)
                                                                 cgcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGa                                                                                  >  6:319599/1‑149 (MQ=255)
                                                                 cgcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGAGCTGTTGATCGTCAAGCCGCTCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGa                                                                                  <  3:59418/149‑1 (MQ=255)
                                                                  gcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCt                                                                                                                          >  3:125584/1‑108 (MQ=255)
                                                                  gcTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCt                                                                                                                          <  4:125584/108‑1 (MQ=255)
                                                                      ggCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAg                                                                           <  1:109738/149‑1 (MQ=255)
                                                                      ggCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAg                                                                           <  7:327464/149‑1 (MQ=255)
                                                                         cATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCtt                                                                        <  3:168331/149‑1 (MQ=255)
                                                                               aTCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTg                                                                   >  7:41759/1‑148 (MQ=255)
                                                                               aTCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTg                                                                   >  5:33036/1‑148 (MQ=255)
                                                                               aTCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTg                                                                   <  3:153382/148‑1 (MQ=255)
                                                                                tCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGa                                                                 <  5:296307/149‑1 (MQ=255)
                                                                                tCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGa                                                                 >  4:63198/1‑149 (MQ=255)
                                                                                 cgcgGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGAt                                                                <  4:359464/149‑1 (MQ=255)
                                                                                  gcgGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGAt                                                                <  3:109031/148‑1 (MQ=255)
                                                                                       aTGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGc                                                          <  4:77723/149‑1 (MQ=255)
                                                                                        tGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGGAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGc                                                          >  4:10460/1‑148 (MQ=255)
                                                                                           gcTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACa                                                      <  4:316582/149‑1 (MQ=255)
                                                                                           gcTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACa                                                      <  1:314672/149‑1 (MQ=255)
                                                                                               aaGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGcc                                                   <  7:266743/148‑1 (MQ=255)
                                                                                               aaGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGc                                                    >  3:200031/1‑147 (MQ=255)
                                                                                                  cTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTg                                               <  3:72722/149‑1 (MQ=255)
                                                                                                  cTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTg                                               <  6:261927/149‑1 (MQ=255)
                                                                                                   tttGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                           >  6:344967/1‑106 (MQ=255)
                                                                                                   tttGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCttt                                                                                           <  5:344967/106‑1 (MQ=255)
                                                                                                      gCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTa                                            <  8:79416/148‑1 (MQ=255)
                                                                                                         ccGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCCCACGATCTTTCGCGATTCCACGCAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCg                                        <  6:165743/149‑1 (MQ=255)
                                                                                                            aTCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCggg                                      >  1:348804/1‑148 (MQ=255)
                                                                                                                gTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAgcg                                 >  2:191298/1‑149 (MQ=255)
                                                                                                                gTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGAACCTTGAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAg                                   >  1:126017/1‑149 (MQ=255)
                                                                                                                    aTCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCAc                             <  3:63198/149‑1 (MQ=255)
                                                                                                                           aGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCAtt                      <  5:384257/149‑1 (MQ=255)
                                                                                                                            gCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCAGCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTg                     <  5:319599/149‑1 (MQ=255)
                                                                                                                              cgcgCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGcc                   >  1:62799/1‑149 (MQ=255)
                                                                                                                                cgcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGa                 <  5:166439/149‑1 (MQ=255)
                                                                                                                                 gcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg                <  5:314970/149‑1 (MQ=255)
                                                                                                                                  ccgcGAGGCCTCACGGTAGGCCTCGCGGGAAATCCCCACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg                >  6:268517/4‑148 (MQ=255)
                                                                                                                                  ccgcGAGGCCTCACGGTAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg                >  2:20382/4‑148 (MQ=255)
                                                                                                                                  cTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAg                >  8:355035/1‑148 (MQ=255)
                                                                                                                                       aGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCgg                                       >  4:243199/1‑120 (MQ=255)
                                                                                                                                       aGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCgg                                       <  3:243199/120‑1 (MQ=255)
                                                                                                                                       aGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATAtt          >  1:166669/1‑149 (MQ=255)
                                                                                                                                               cGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCg  <  2:166669/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGGGCTCGCCCGACCATCGCGGGGTAATCGCCACCTGCAACTATGAAGCGCGTGCCTATGGTGTGCGCTCGGCCATGTCATCGCGGCATGCGCTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGAC‑‑GAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCG  >  NC_002947/1381350‑1381641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑