Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,416,862	G→A	7.6%	R37Q (CGG→CAG)	PP_1239 →	metallo‑beta‑lactamase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,416,862	0	G	A	7.6%	195.8 / 4.5	69	R37Q (CGG→CAG)	PP_1239	metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base A (3/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

CGTGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCAT  >  NC_002947/1416719‑1417009                                                                                                                                                |                                                                                                                                                     cgTGCTGAGCGCTATTCAGGACCACCTTGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGcc                                                                                                                                                 <  2:120440/149‑1 (MQ=255) cgTGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGcc                                                                                                                                                 <  2:72355/149‑1 (MQ=255)      tGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGgcg                                                                                                                                            >  2:72380/1‑149 (MQ=255)        aGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCgct                                                                                                                                          <  4:309297/149‑1 (MQ=255)        aGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGgcgc                                                                                                                                           <  5:191697/148‑1 (MQ=255)          cgcTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAGGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCgctgc                                                                                                                                        >  8:312021/1‑149 (MQ=255)          cgcTATGCAGGACCACCTGGGCGAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCgctgc                                                                                                                                        <  8:163266/149‑1 (MQ=255)             tATTCAGGACCCCCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCg                                                                                                                                     >  4:283950/1‑149 (MQ=255)             tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTc                                                                                                                                      >  8:324987/1‑148 (MQ=255)               ttCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTc                                                                                                                                      <  7:324987/146‑1 (MQ=255)               ttCAGGACCACCTGCGCTAAGTGCGCTTCACGGTACTCGGAAGCGGCAGCCAAGGGAACGCCACGATGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTCCTCggc                                                                                                                                   >  2:168534/1‑148 (MQ=255)                   ggACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGttt                                                                                                                               >  3:196908/1‑149 (MQ=255)                    gACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTc                                                                                                                              >  5:256116/1‑149 (MQ=255)                     accaccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCt                                                                                                                             <  5:341179/149‑1 (MQ=255)                      ccaccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTg                                                                                                                            <  3:75600/149‑1 (MQ=255)                          cTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCATCCAAGGGAACGCCACGCTGATCGCAAGTGGTAACACGGTCATCCTCGTAGATTGCGGCTTTTCACTGAGTGAAACCTAGAGGCGCATGGCACTGCTCGGGATTTCTGcagc                                                                                                                        >  6:290430/1‑149 (MQ=255)                          cTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagc                                                                                                                        >  4:121159/1‑149 (MQ=255)                                  aGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctcagct                                                                                                                  <  6:294025/147‑1 (MQ=255)                                   gTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAAAGGCACGCTGATCGCCAGTGGTGACACGTTCATCATGGTCGAGTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGctcagctcag                                                                                                               >  6:123707/1‑149 (MQ=255)                                   gTGCGCTTCGCGGGACGCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCGGTGGGGACACGTTCATCCGGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAACGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGctcagctcag                                                                                                               <  7:179908/149‑1 (MQ=255)                                    tGCGCTTCGCGGTACTCGGAAGCGGCAGCCAGGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagt                                                                                                              <  7:312021/149‑1 (MQ=255)                                    tGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagt                                                                                                              <  2:279175/149‑1 (MQ=255)                                        cTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcag                                                                                                          >  7:198771/1‑149 (MQ=255)                                             cgGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                     <  2:359913/149‑1 (MQ=255)                                                  cTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCa                                                                                                 <  2:160375/148‑1 (MQ=255)                                                   tCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCAcc                                                                                               >  2:267695/1‑149 (MQ=255)                                                     ggAAGCGGCAGCCAAGGGAACGGCAAGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCAcc                                                                                               <  6:174297/147‑1 (MQ=255)                                                      gAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCAc                                                                                            <  3:121159/149‑1 (MQ=255)                                                      gAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCAc                                                                                            >  3:298198/1‑149 (MQ=255)                                                               gCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGcc                                                                                   <  4:196908/149‑1 (MQ=255)                                                                 cAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAGCATGCCGa                                                                                 >  8:159553/1‑149 (MQ=255)                                                                     ggAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCAc                                                                             >  3:87787/1‑149 (MQ=255)                                                                       aaCGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGGCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGt                                                                           <  2:21910/149‑1 (MQ=255)                                                                             aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATgg                                                                    <  6:256116/149‑1 (MQ=255)                                                                                  gATCGCCAGTGGTGACACGTTCATCCTGGTCGATAGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCCCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGC‑ACGTGGTCg                                                               >  1:50849/1‑149 (MQ=255)                                                                                   aTCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCgg                                                              >  2:3104/1‑149 (MQ=255)                                                                                    tCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGC‑ACGTGGTCGGc                                                             <  8:38398/149‑1 (MQ=255)                                                                                      gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTc                                                                                                  <  2:129650/111‑1 (MQ=255)                                                                                      gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGATTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTCCAGTGCTGGTc                                                                                                  >  1:129650/1‑111 (MQ=255)                                                                                       ccAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTg                                                          >  2:48684/1‑149 (MQ=255)                                                                                         aGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCt                                                        <  7:159553/149‑1 (MQ=255)                                                                                         aGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCt                                                        <  4:298198/149‑1 (MQ=255)                                                                                             gtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGt                                                      <  8:338396/147‑1 (MQ=255)                                                                                             gtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGt                                                      >  7:338396/1‑147 (MQ=255)                                                                                             gtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTc                                                     <  1:205204/148‑1 (MQ=255)                                                                                             gtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCa                                                    >  3:102432/1‑149 (MQ=255)                                                                                                  acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCa                                                    <  4:368428/144‑1 (MQ=255)                                                                                                  acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCa                                                    >  3:368428/1‑144 (MQ=255)                                                                                                    gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCt                                                        >  8:213940/1‑138 (MQ=255)                                                                                                    gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCt                                                        <  7:213940/138‑1 (MQ=255)                                                                                                    gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGgcgc                                              >  2:327807/1‑148 (MQ=255)                                                                                                         tCCTGGTGGATTGCGGCTTTTCCCTGCGTGAAAGCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAAt                                        <  1:168534/149‑1 (MQ=255)                                                                                                         tCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAAt                                        <  4:196754/149‑1 (MQ=255)                                                                                                              gTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACTAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAcc                                   <  3:283950/149‑1 (MQ=255)                                                                                                                   ttGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTc                                                                <  8:151495/115‑1 (MQ=255)                                                                                                                   ttGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTc                                                                >  7:151495/1‑115 (MQ=255)                                                                                                                     gCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAc                            >  2:342736/1‑149 (MQ=255)                                                                                                                      cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCAATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAc                            <  1:72380/149‑1 (MQ=255)                                                                                                                      cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAcc                           >  3:330806/1‑149 (MQ=255)                                                                                                                      cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAc                            <  1:3104/148‑1 (MQ=255)                                                                                                                        gCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCt                          >  2:287686/1‑148 (MQ=255)                                                                                                                            ttCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGc                      <  8:198771/148‑1 (MQ=255)                                                                                                                              cccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGcc                     >  5:135608/1‑147 (MQ=255)                                                                                                                               ccTGCGTGAAACCGAGCCGCGCCTGGCGCTGCTCGGTGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGAACACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAg                  <  5:290430/149‑1 (MQ=255)                                                                                                                                    gaaaccccGAGCCGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCCCCCACGCACATGCCGCCCACGTGC‑ATGGGGTCGGCTGGCTGTCACGGCGCTACAATGTACCGGTCTACCTCCGCCAAGGGACt             >  5:41686/7‑149 (MQ=255)                                                                                                                                      gAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACttt           >  4:309481/1‑149 (MQ=255)                                                                                                                                         cccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg        >  7:282419/2‑149 (MQ=255)                                                                                                                                         cccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg        >  4:340783/2‑149 (MQ=255)                                                                                                                                           cGAGCGGCGCCTGGCGCTGCGCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGGCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGcgcg      <  5:123707/149‑1 (MQ=255)                                                                                                                                               cggcgCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCAt  <  1:48684/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CGTGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGC‑ATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCAT  >  NC_002947/1416719‑1417009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑