| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,433,030 | T→A | 12.9% | intergenic (+18/+106) | xenA → / ← PP_1255 | protein XenA/cis‑4‑hydroxy‑D‑proline oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,433,030 | 0 | T | A | 12.9% | 138.1 / 11.2 | 54 | intergenic (+18/+106) | xenA/PP_1255 | protein XenA/cis‑4‑hydroxy‑D‑proline oxidase |
| Reads supporting (aligned to +/- strand): ref base T (20/27); new base A (3/4); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCC > NC_002947/1432884‑1433163 | gTTTCGGTAGGGCGCGCGCACCTGGCCGACGCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTg < 6:323727/149‑1 (MQ=255)gTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTg > 6:279068/1‑149 (MQ=255)gTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTg < 2:110790/149‑1 (MQ=255) tCGGTAGGGCGCGCGCACCTGGCGGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCa < 3:111035/148‑1 (MQ=255) tCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCa < 1:84357/148‑1 (MQ=255) ggTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGc < 1:108750/148‑1 (MQ=255) tAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCAAAGCAGcccc < 3:72712/149‑2 (MQ=255) gcgcgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTcg > 6:100639/1‑149 (MQ=255) cgcgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCAAAGCAGcccctccaa < 2:293580/149‑7 (MQ=255) gcgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTcgcg < 4:178622/149‑1 (MQ=255) cgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCgg < 4:168122/149‑1 (MQ=255) cgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCgg < 4:334029/149‑1 (MQ=255) cgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGc < 7:224378/149‑1 (MQ=255) cACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCaa < 5:100639/149‑1 (MQ=255) ccGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCca > 7:98815/1‑148 (MQ=255) ccGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGCAAAAAGCCTCCTGGACATTGCCGGCGCCTTATGCACACTGGCTCGAGCGATATCGCTGAGGCTTGGAGCGCCTGATTTGCAGCCCAATCGCGGGCAAGCCCGCACCca > 7:320257/1‑148 (MQ=255) gCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCAc < 3:161230/136‑1 (MQ=255) gCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCAc > 4:161230/1‑136 (MQ=255) cTTACTTTGCTGTCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCg > 5:122997/1‑149 (MQ=255) aCTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGGCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCa < 8:98815/148‑1 (MQ=255) tgctgcCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCa < 3:357895/104‑1 (MQ=255) tgctgcCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCa > 4:357895/1‑104 (MQ=255) ctgcCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCa < 3:357435/149‑1 (MQ=255) tgcCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGttt > 7:260991/1‑146 (MQ=255) aaGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGGTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATAc > 8:112153/1‑149 (MQ=255) aaGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGa < 3:291326/146‑1 (MQ=255) aaGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGa > 4:291326/1‑146 (MQ=255) aaGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATAc > 4:104387/1‑149 (MQ=255) aGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCa < 2:308059/149‑1 (MQ=255) cTGGGGGTGTAAAAAGCCTCCTGGACCTGGCCGGAGCCTTATGCACACTGGATCGAGATTTATCTCTGAGGCTTGGAGGGGCTGCTTTGCAGCCAATTCTCTTGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCAcc < 1:76257/149‑1 (MQ=255) ggggTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACagag < 3:76508/120‑1 (MQ=255) ggggTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACagag > 4:76508/1‑120 (MQ=255) ggTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCctctc > 8:59400/1‑149 (MQ=255) gTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCctctct < 5:279068/149‑1 (MQ=255) gAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgtg > 6:152882/1‑149 (MQ=255) gAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgt < 6:122997/148‑1 (MQ=255) aaaaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACt > 4:351585/1‑134 (MQ=255) aaaaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACt < 3:351585/134‑1 (MQ=255) aaaaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgtg > 8:33414/1‑148 (MQ=255) gACCTTGCCGGCGCGTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGTAGGGGCTGCTTTTCAGCACATTCGCGGGCAAGCCCGCAACCACAGAGACACCGCAGGTTTAATATACTGCACCCTCTCTGTGGAAGTGGGCTTGc < 8:320257/148‑1 (MQ=255) gACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGc < 3:104387/148‑1 (MQ=255) cGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATTCTGCACCCACTCTCTGGAAGTGGGCTTGCCCGCGAATg > 2:198476/1‑149 (MQ=255) cacaCTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAggg < 8:260991/148‑1 (MQ=255) acacTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGc > 7:1248/1‑149 (MQ=255) aTCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATAGGGCCCCCTCTCTGTGGAAGTGCGCTTGCACGCGAAAGGGCCGCTACGGGCGAATACCGGTTCAGTCg > 7:333858/1‑149 (MQ=38) aGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATgc < 5:152882/148‑1 (MQ=255) aGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCGAAAACCGGTTCAGTCGGTATgc < 7:59400/148‑1 (MQ=255) gCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCgg < 4:281405/141‑1 (MQ=255) gCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCgg > 3:281405/1‑141 (MQ=255) gcgaatgggCTGCAAAGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGCCACCGCAGGTTTCAGATACTGCCCCCGCTCTGAGGCACTGGGCTAGACCGCGAATGGTCCGGTAGGGGCTAAAACCGGTGCAGGCGGTATGCGccc > 1:285782/7‑148 (MQ=255) gaatgggCTGCAAAGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGt > 8:287283/5‑138 (MQ=255) gaatgggCTGCAAAGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGt < 7:287283/134‑1 (MQ=255) gaatgggCTGCAAAGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGccc < 2:139539/142‑1 (MQ=255) gaatgggCTGCAAAGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGccc > 1:139539/5‑146 (MQ=255) | GTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCC > NC_002947/1432884‑1433163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |