Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,463,025	A→T	9.9%	intergenic (‑101/+41)	algI ← / ← algL	alginate O‑acetylase AlgI/alginate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,463,025	0	A	T	9.9%	139.8 / 5.6	58	intergenic (‑101/+41)	algI/algL	alginate O‑acetylase AlgI/alginate lyase
Reads supporting (aligned to +/- strand):  ref base A (27/24);  new base T (3/3);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

TCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCGTGTCGCCAA  >  NC_002947/1462882‑1463172                                                                                                                                                |                                                                                                                                                     tCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAACCCCCCCCGTGCAAGGCTGGAAGGCAACGTAGCTCCCTGTGGGAGCGGGCAAGCCCg                                                                                                                                                 >  2:278581/1‑149 (MQ=255)   ggCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGGAAGGCGGGAAGGCAACTTAGATACCAGTGGGGGCGGGCAAGCCCGca                                                                                                                                               >  8:336117/1‑148 (MQ=255)       aGAACAGGAACAGGAACACGTTGGACGAGAAGACCAACCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCACGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCca                                                                                                                                           <  5:206631/149‑1 (MQ=255)             cgaacaggaacaCGTTGGACGACAAGACCAACCCGGTGCTCCTAATTTGTTCACAGCATCCAGCGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGAGCCCTGTGGGAGCGGGCTTGCCCGCTCCCACAggga                                                                                                                                     <  1:344802/148‑3 (MQ=255)                          gTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTCCACAGCATCCAGGGCAAAGCGCCCCCCAACCCCCCCAGTGGCAGGCTGGAAGGCCACTTAGATACCTGTGGGAGGGGGCAAGCCCGCGCCCGCAGGATCCGCCCCCGcc                                                                                                                          >  8:327784/1‑146 (MQ=255)                           ttGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAGCCCCCCCCTGAAAGGCTGGCCGGGCAAGTAGAACCCTGTGGGCGCGGGCAAGCCCGCACCCACAGGTTGCGCCCCCGCAATg                                                                                                                       >  6:23834/1‑149 (MQ=255)                             ggACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATgg                                                                                                                      <  5:134041/148‑1 (MQ=255)                               aCGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCACACCCCCCAGTGCAAGGGTGGAAGGCGACGTAGATCCCTGTGGGAGCGGGGAAGCCCGCGCCCACAGGGTCCGCCCCCCCAAAggggg                                                                                                                   >  2:239767/1‑149 (MQ=255)                                cGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAATCCCCGCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGt                                                                                                                  <  5:255622/149‑1 (MQ=255)                                 gagaAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTc                                                                                                                 <  6:68221/149‑1 (MQ=255)                                           tCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCACGGCTGGAAGGCAACTTAGATCCCTGTGGGGGCGGGCACGCCCGGGCCCCCAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTc                                                                                                       >  8:198991/1‑149 (MQ=255)                                             ccGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCtt                                                                                                     <  3:235786/149‑1 (MQ=255)                                              cGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTg                                                                                                    <  7:61455/149‑1 (MQ=255)                                                      cTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCCAACCCCCCAGTGCAAGGCGGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCCCCGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGGTCTCTg                                                                                             >  8:168191/1‑148 (MQ=255)                                                              ttCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCAGTGGGAGCGGGCAAGCCCGCTCCCCCAGGTTCCGCCCCCCCAATGGGGGTCAATTAATTTCTTGAGGTGTGCACACGGGt                                                                                    >  8:339623/1‑149 (MQ=255)                                                                   aGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGctct                                                                                                <  3:348328/132‑1 (MQ=255)                                                                          aGGGCAAAGCGCCCCCCCAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCCAGCCCGCGCCCCCCGGGTCCGCCCCCGCAATGGGGGGCAATTCATTTGTTGCTGTCTGCACTCGGGTCGTAGCCCcgcg                                                                        >  6:148245/1‑149 (MQ=255)                                                                                        cccAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCgccgcc                                                          >  3:86477/1‑148 (MQ=255)                                                                                            aaCCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGcc                                                     >  7:120554/1‑149 (MQ=255)                                                                                               cccccAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCgg                                                   >  3:17138/1‑148 (MQ=255)                                                                                               cccccAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGa                                                  <  7:168191/149‑1 (MQ=255)                                                                                                   cAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCt                                              <  5:134049/149‑1 (MQ=255)                                                                                                        cAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTg                                          >  5:363919/1‑148 (MQ=255)                                                                                                         aaGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGaa                                        >  7:13033/1‑149 (MQ=255)                                                                                                            gCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCgg                                                                                      >  8:298971/1‑101 (MQ=255)                                                                                                            gCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCgg                                                                                      <  7:298971/101‑1 (MQ=255)                                                                                                             cTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGc                                    <  8:114218/149‑1 (MQ=255)                                                                                                              tGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCt                                   >  1:63938/1‑149 (MQ=255)                                                                                                                 caGGCAACTTATTTCCCTTTTGGAGCGGGCAAGCCCGCTGCCAAATGTTCCGGCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGGCGTAGACCCGGGTCACCATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTg                                  <  1:239767/147‑1 (MQ=255)                                                                                                                 aaGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCa                                >  4:124505/1‑149 (MQ=255)                                                                                                                     cAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTc                            <  7:143341/149‑1 (MQ=255)                                                                                                                      aaCTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCg                           <  4:12848/149‑1 (MQ=255)                                                                                                                       aCTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGCCCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTGGAACGGCTGCATGTCGt                          >  5:348648/1‑149 (MQ=255)                                                                                                                        cTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTg                         <  6:363919/149‑1 (MQ=255)                                                                                                                            ggaaccTGTGGGAGCGGGCTTGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTg                     >  5:98965/5‑149 (MQ=255)                                                                                                                            gATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCtt                      <  8:13033/148‑1 (MQ=255)                                                                                                                            gATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGACAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCtt                      <  5:131256/148‑1 (MQ=255)                                                                                                                             aTCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGaa                                        <  5:61229/129‑1 (MQ=255)                                                                                                                             aTCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGaa                                        >  6:61229/1‑129 (MQ=255)                                                                                                                               cccTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTgcgc                  >  3:127531/1‑149 (MQ=255)                                                                                                                                ccTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGCCCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCt                 >  3:85124/1‑149 (MQ=255)                                                                                                                                ccTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTgcgc                  <  7:339623/148‑1 (MQ=255)                                                                                                                                 cTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGc                                 <  3:156024/132‑1 (MQ=255)                                                                                                                                 cTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGc                                 >  4:156024/1‑132 (MQ=255)                                                                                                                                  tgtgGGAGCGGGCTTGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGt                                                                       <  8:299550/94‑1 (MQ=255)                                                                                                                                  tgtgGGAGCGGGCTTGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGt                                                                       >  7:299550/1‑94 (MQ=255)                                                                                                                                    tgGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCaa              <  5:97875/148‑1 (MQ=255)                                                                                                                                      ggAGCGGGCTTGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCg           >  4:8769/1‑149 (MQ=255)                                                                                                                                       gAGCGGGCAAGCCCGCTCCCACATGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGATCGGCTGCATGTCGTGCTTGCGCTCAAGCgt          <  4:17138/149‑1 (MQ=255)                                                                                                                                        aGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCt                                   >  3:99982/1‑123 (MQ=255)                                                                                                                                        aGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCt                                   <  4:99982/123‑1 (MQ=255)                                                                                                                                        aGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCgtg         >  4:136131/1‑149 (MQ=255)                                                                                                                                         gCGGGCTTGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCgtgt        <  3:8769/149‑1 (MQ=255)                                                                                                                                         gCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCgtgt        <  7:327784/149‑1 (MQ=255)                                                                                                                                          cGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGAACGCGGGTCGTAGACCCGCGTCC‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGATTGCGCTCAAGCGTGTc       >  2:12264/1‑149 (MQ=255)                                                                                                                                           gggCAAGCCCGCTCACAAAGGTTCCGCCACCGCAATGGTGGTCAATTCATTTCTTGCTCTCTGCAATCGGGTCGAATACAAGCTTCA‑CATATCCGCCCAGCCCTAACCTCTTTCAAGGCTGCAAGTCGTGCTTGAGCTAAAGCGTGTCg      >  4:142425/1‑149 (MQ=255)                                                                                                                                             gCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCGTGTCGc     >  5:108034/1‑148 (MQ=255)                                                                                                                                             gCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCGTGTCGc     >  5:115522/1‑148 (MQ=255)                                                                                                                                               aaGCCCGCTCCCACAGGTTCCGCCCCCGCATTGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATAGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCACTCGTGTCGCCaa  >  3:237225/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                     TCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCA‑CATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCGTGTCGCCAA  >  NC_002947/1462882‑1463172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑