Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,469,817 C→T 9.5% R109H (CGC→CAC)  algK ← alginate biosynthesis protein AlgK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,469,8170CT 9.5% 175.6 / 5.9 63R109H (CGC→CAC) algKalginate biosynthesis protein AlgK
Reads supporting (aligned to +/- strand):  ref base C (32/25);  new base T (3/3);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGCCAGCGC  >  NC_002947/1469675‑1469965
                                                                                                                                              |                                                                                                                                                       
ggggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTGTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑cc                                                                                                                                                    >  6:139645/1‑147 (MQ=255)
 gggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCaa                                                                                                                                                  <  6:125335/148‑1 (MQ=255)
  ggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAgg                                                                                                                                                <  3:70378/149‑1 (MQ=255)
  ggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGCCTTGGCCGCCTGCTGGTGG‑CCAAgc                                                                                                                                                >  3:277601/1‑148 (MQ=255)
  ggAAACTCTGCGGGTAGCTGAGGTAGAGCAGTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCGCCTGCTGGTGG‑CCAAgc                                                                                                                                                <  5:215988/149‑2 (MQ=255)
   gAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGCCTTGGCCGCCTGCTGGTGG‑CCAAgcc                                                                                                                                               <  4:277601/149‑3 (MQ=255)
   gAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGCCTTGGCCGCCTGCTGGTGG‑CCAAgcc                                                                                                                                               <  3:47967/149‑3 (MQ=255)
                  gCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGt                                                                                                                               <  5:139645/148‑1 (MQ=255)
                   cTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTgg                                                                                                                             >  7:186647/1‑149 (MQ=255)
                    tGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGc                                                                                                                            >  7:184357/1‑149 (MQ=255)
                    tGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGc                                                                                                                            >  5:199412/1‑149 (MQ=255)
                      aGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGc                                                                                                                            <  4:297393/147‑1 (MQ=255)
                              cATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTggcgg                                                                                                                  >  3:273072/1‑149 (MQ=255)
                              cATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTACTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTggcgg                                                                                                                  >  7:144076/1‑149 (MQ=255)
                                ttGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGcc                                                                                                                <  3:198197/149‑1 (MQ=255)
                                   ccAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCg                                                                                                               >  3:254573/1‑147 (MQ=255)
                                   ccAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCa                                                                                                             <  6:199412/149‑1 (MQ=255)
                                      gCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAgcg                                                                                                          >  5:118416/1‑149 (MQ=255)
                                           aTCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATa                                                                                                     <  4:254573/149‑1 (MQ=255)
                                               gggTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTg                                                                                                 >  5:327117/1‑149 (MQ=255)
                                               gggTATTGCTTTGGCCCTGTTTCGCGGCCTACTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTg                                                                                                 <  8:144076/149‑1 (MQ=255)
                                                 gTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGc                                                                                               >  8:53555/1‑149 (MQ=255)
                                                    ttGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTc                                                                                            <  8:186647/149‑1 (MQ=255)
                                                      gCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCg                                                                                           <  7:53555/148‑1 (MQ=255)
                                                            gCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCt                                                                                    <  6:327117/149‑1 (MQ=255)
                                                            gCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCt                                                                                    >  7:2517/1‑149 (MQ=255)
                                                                    tCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCg                                                                                                               <  3:46037/114‑1 (MQ=255)
                                                                    tCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCg                                                                                                               >  4:46037/1‑114 (MQ=255)
                                                                     cgcgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGc                                                                            <  8:2517/148‑1 (MQ=255)
                                                                       cgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCgg                                                                                          <  7:284674/132‑1 (MQ=255)
                                                                       cgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCgg                                                                                          >  8:284674/1‑132 (MQ=255)
                                                                         gCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAggg                                                                       <  6:118416/149‑1 (MQ=255)
                                                                          ccTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAgggg                                                                      >  2:345658/1‑149 (MQ=255)
                                                                              cTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTcgc                                                                  >  1:241027/1‑149 (MQ=255)
                                                                                   gCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTg                                                             <  4:273072/149‑1 (MQ=255)
                                                                                    cAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTgg                                                            >  2:362226/1‑149 (MQ=255)
                                                                                     aGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGc                                                           >  1:358627/1‑149 (MQ=255)
                                                                                      gggTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCgg                                                                >  5:2240/1‑143 (MQ=255)
                                                                                      gggTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCgg                                                                <  6:2240/143‑1 (MQ=255)
                                                                                         tttCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCGGTGTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCa                                                         >  1:298765/1‑149 (MQ=255)
                                                                                             ggCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTg                                                    <  4:365125/148‑1 (MQ=255)
                                                                                              gCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGAt                                                  >  5:154273/1‑149 (MQ=255)
                                                                                              gCTTCCTCAAGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGTCGGCCCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGAGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTAGCGGGTGGCCACCTTGa                                                   >  1:284698/1‑149 (MQ=255)
                                                                                                   cTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTcc                                              <  3:79130/148‑1 (MQ=255)
                                                                                                   cTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCg                                             >  2:13365/1‑149 (MQ=255)
                                                                                                    tCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGTCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCCTGATGTCCGc                                            >  5:256340/1‑149 (MQ=255)
                                                                                                    tCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGc                                            >  3:208415/1‑149 (MQ=255)
                                                                                                     cACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGa                                                                          >  7:89274/1‑118 (MQ=255)
                                                                                                     cACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGa                                                                          <  8:89274/118‑1 (MQ=255)
                                                                                                     cACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGc                                            >  1:163003/1‑148 (MQ=255)
                                                                                                         ttCGGCCTGGGGGCTGTCGGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGAGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGc                                       <  2:290594/149‑1 (MQ=255)
                                                                                                             gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGAc                                   >  3:120498/1‑149 (MQ=255)
                                                                                                             gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGC‑G‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTg                                                                             >  7:233434/1‑106 (MQ=255)
                                                                                                             gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGC‑G‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTg                                                                             <  8:233434/106‑1 (MQ=255)
                                                                                                                     gCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCtt                           >  6:206706/1‑149 (MQ=255)
                                                                                                                         tCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCt                       <  8:184357/149‑1 (MQ=255)
                                                                                                                           cGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTg                      >  4:50473/1‑148 (MQ=255)
                                                                                                                            ggCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTa                    <  2:298765/149‑1 (MQ=255)
                                                                                                                             ccTTGGCCGCCTGCTGGTGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAg                   >  1:78733/2‑149 (MQ=255)
                                                                                                                              cTTGGCCGCCTGCTGGTGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGc                  >  3:315389/1‑149 (MQ=255)
                                                                                                                                   ccaccaGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAgg             >  4:164361/1‑149 (MQ=255)
                                                                                                                                      ccaGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCg          <  1:362226/149‑1 (MQ=255)
                                                                                                                                           agGCGG‑CCAAGGCGCG‑‑CTTGGGTGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGCCAg     >  4:265014/1‑149 (MQ=255)
                                                                                                                                             gcgg‑cCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACCTGGGCTACAGCGAAGCGCAAGTCGGcctggc   <  1:345658/149‑5 (MQ=37)
                                                                                                                                              cgg‑cCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTTGCTGTAGCCCAGGTCGGCCAgcgc  <  7:233091/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                       
GGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGG‑CCAAGGCGCG‑‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGCCAGCGC  >  NC_002947/1469675‑1469965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑