Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,496,383 C→A 8.8% A271D (GCC→GAC) ‡ mdeA → methionine gamma‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,496,3830CA 8.8% 150.9 / 2.7 52A271D (GCC→GAC) ‡mdeAmethionine gamma‑lyase
Reads supporting (aligned to +/- strand):  ref base C (27/20);  new base A (2/3);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAGGGT  >  NC_002947/1496235‑1496531
                                                                                                                                                    |                                                                                                                                                    
gggTTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGc                                                                                                                                                      <  5:110577/149‑1 (MQ=255)
  gTTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGcc                                                                                                                                                     >  6:191922/1‑148 (MQ=255)
       tggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGcc                                                                                                                                               <  2:48489/149‑1 (MQ=255)
        ggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccg                                                                                                                                              <  8:94607/149‑2 (MQ=255)
        gggggggCGCAAGCGGATGGGCGATCCCATTCGCCGCGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccg                                                                                                                                              <  3:199229/146‑2 (MQ=255)
         gtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGcc                                                                                                                                               >  6:137064/1‑147 (MQ=255)
           ggggCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccg                                                                                                                                              <  5:85053/146‑2 (MQ=255)
           ggggCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccg                                                                                                                                              >  6:85053/1‑145 (MQ=255)
                  aaGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCcagca                                                                                                                                          <  8:92040/143‑1 (MQ=255)
                  aaGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCcagca                                                                                                                                          >  7:92040/1‑143 (MQ=255)
                        ttGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGtt                                                                                                                              >  5:137313/1‑149 (MQ=255)
                          ggTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTtc                                                                                                                            >  7:247710/1‑149 (MQ=255)
                           gTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTtc                                                                                                                            <  3:211469/148‑1 (MQ=255)
                            tCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCTCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTtctc                                                                                                                          <  7:252044/149‑1 (MQ=255)
                            tCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTtctc                                                                                                                          <  1:150517/149‑1 (MQ=255)
                              gATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                        >  1:19295/1‑149 (MQ=255)
                                   cATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCgg                                                                                                                    >  2:109478/1‑148 (MQ=255)
                                     ttCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAg                                                                                                                 >  5:153220/1‑149 (MQ=255)
                                      tCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGc                                                                                                                >  2:173811/1‑149 (MQ=255)
                                          cTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCg                                                                                                              >  8:283307/1‑147 (MQ=255)
                                              aaGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTg                                                                                                        <  7:283307/149‑1 (MQ=255)
                                                  gCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAAt                                                                                                    <  1:173811/149‑1 (MQ=255)
                                                      aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGAt                                                                                                <  3:91773/149‑1 (MQ=255)
                                                      aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCAGTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGAt                                                                                                >  7:160478/1‑149 (MQ=255)
                                                        ggATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGAtt                                                                                               >  7:39814/1‑148 (MQ=255)
                                                           tatGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCAtt                                                                                           <  6:137313/149‑1 (MQ=255)
                                                              gACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGGCGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGTGTTTATCGTCCGGCAGCCGCAGGTGGAATTGATTCATTAcc                                                                                        >  1:164675/1‑149 (MQ=255)
                                                                 cGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCgg                                                                                     >  7:24728/1‑149 (MQ=255)
                                                                  ggggCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCggg                                                                                    <  6:348380/149‑1 (MQ=255)
                                                                           cTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTc                                                                           <  1:109478/149‑1 (MQ=255)
                                                                             gTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGAAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCggatt                                                                         >  4:29871/1‑145 (MQ=255)
                                                                               cGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCtttt                                                                       <  5:191922/149‑1 (MQ=255)
                                                                                gCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTg                                                                      >  4:76679/1‑149 (MQ=255)
                                                                                  cccATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGcc                                                                    >  4:276189/1‑149 (MQ=255)
                                                                                  cccATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGATACAGTCTTTTGcc                                                                    >  6:291563/1‑149 (MQ=255)
                                                                                     aTGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGccc                                                                   >  2:114318/1‑147 (MQ=255)
                                                                                     aTGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGccc                                                                   <  1:114318/147‑1 (MQ=255)
                                                                                       gACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                              >  8:220105/1‑118 (MQ=255)
                                                                                       gACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                              <  7:220105/118‑1 (MQ=255)
                                                                                                   ctgATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCa                                                   <  3:359699/149‑1 (MQ=255)
                                                                                                    tgATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAg                                                  >  3:204451/1‑149 (MQ=255)
                                                                                                              gATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGc                                        >  1:124570/1‑149 (MQ=255)
                                                                                                                  aaGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGttt                                    <  5:137064/149‑1 (MQ=255)
                                                                                                                  aaGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGGCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGgtt                                    >  8:275224/1‑149 (MQ=255)
                                                                                                                   aGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTg                                   >  4:196335/1‑149 (MQ=255)
                                                                                                                               tgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCggg                          >  3:309811/1‑146 (MQ=255)
                                                                                                                               tgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCggg                          <  4:309811/146‑1 (MQ=255)
                                                                                                                                    aTGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGc                  >  8:290001/1‑149 (MQ=255)
                                                                                                                                      ggACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCt                >  3:269786/1‑149 (MQ=255)
                                                                                                                                        aCCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCttt              <  2:111720/149‑1 (MQ=255)
                                                                                                                                        aCCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTGTTGCCCAGTACGCGTTGGCGCCGCGACAGATGCGGTTGCCTGGCGGGCTGATTGCCttg              >  4:208718/1‑148 (MQ=255)
                                                                                                                                         tgggCATTGGCACAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGACTGGCGGGATGATTGCCttt              >  8:288060/3‑148 (MQ=255)
                                                                                                                                          cGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGa            <  3:76679/149‑1 (MQ=255)
                                                                                                                                          cGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGa            <  8:39814/149‑1 (MQ=255)
                                                                                                                                                    ccAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAGGGt  >  4:285266/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGTTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAGGGT  >  NC_002947/1496235‑1496531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑