Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,537,752 A→T 6.8% P253P (CCA→CCT)  PP_1348 → MutT/nudix family protein/thiamine‑phosphate pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,537,7520AT 6.8% 220.6 / 4.8 73P253P (CCA→CCT) PP_1348MutT/nudix family protein/thiamine‑phosphate pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base A (30/38);  new base T (2/3);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

TTGGCACCTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCGGGGCACA  >  NC_002947/1537605‑1537898
                                                                                                                                                   |                                                                                                                                                  
ttGGCACCTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAg                                                                                                                                                   <  1:147539/149‑1 (MQ=255)
     aCCTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAg                                                                                                                                              <  4:87706/149‑1 (MQ=255)
      ccTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAgg                                                                                                                                             <  1:286612/149‑1 (MQ=255)
       cTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGCGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGc                                                                                                                                            >  3:294474/1‑149 (MQ=255)
         gACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGa                                                                                                                                          >  4:6066/1‑149 (MQ=255)
           cGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGAcc                                                                                                                                        <  6:378185/149‑1 (MQ=255)
            ggCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcag                                                                                                                                       <  6:350502/149‑3 (MQ=255)
                 cgcAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACt                                                                                                                                  <  1:96023/149‑1 (MQ=255)
                    aGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCAc                                                                                                                               >  7:2731/1‑149 (MQ=255)
                    aGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCAc                                                                                                                               >  5:135816/1‑149 (MQ=255)
                            aaGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGc                                                                                                                       >  7:302156/1‑149 (MQ=255)
                            aaGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGc                                                                                                                       >  2:227330/1‑149 (MQ=255)
                             aGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCg                                                                                                                      <  4:56052/149‑1 (MQ=255)
                               tACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcagg                                                                                                                                      >  1:41254/1‑128 (MQ=255)
                               tACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcagg                                                                                                                                      <  2:41254/131‑4 (MQ=255)
                                 cgccgcCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTAACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGTCGGTGc                                                                                                                  >  2:126765/1‑149 (MQ=255)
                                  gccgccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGcc                                                                                                                 >  6:256239/1‑149 (MQ=255)
                                    cgccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGt                                                                                                               <  1:227330/149‑1 (MQ=255)
                                     gccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGCCTCACCCAGAGGCGGTGCCGtt                                                                                                              >  8:58025/1‑149 (MQ=255)
                                     gccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGtt                                                                                                              <  6:347855/149‑1 (MQ=255)
                                     gccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGtt                                                                                                              <  8:306784/149‑1 (MQ=255)
                                      ccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtg                                                                                                             <  7:127848/149‑1 (MQ=255)
                                         ggggCCGACCGTTCCCCAAGGAGGGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGTGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtggta                                                                                                          <  7:102012/149‑3 (MQ=255)
                                         ggggCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtggga                                                                                                          <  7:102010/149‑1 (MQ=255)
                                            gCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatgg                                                                                                       <  5:256239/149‑1 (MQ=255)
                                             ccGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggg                                                                                                      <  3:6066/149‑1 (MQ=255)
                                             ccGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggg                                                                                                      <  8:47654/149‑1 (MQ=255)
                                              cGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggga                                                                                                     <  8:117999/149‑1 (MQ=255)
                                              cGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggga                                                                                                     <  4:363065/149‑1 (MQ=255)
                                               gACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTgggatgggat                                                                                                    <  6:132782/149‑1 (MQ=255)
                                                  cGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAg                                                                                                 <  7:298640/149‑1 (MQ=255)
                                                   gTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAgg                                                                                                >  3:118894/1‑149 (MQ=255)
                                                       cccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtg                                                                                                             <  3:340153/132‑1 (MQ=255)
                                                       cccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtg                                                                                                             >  4:340153/1‑132 (MQ=255)
                                                        ccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAg                                                                                           <  4:326861/149‑1 (MQ=255)
                                                        ccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAg                                                                                           >  8:100517/1‑149 (MQ=255)
                                                        ccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAg                                                                                           >  4:66761/1‑149 (MQ=255)
                                                         cAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGc                                                                                          >  3:322052/1‑149 (MQ=255)
                                                               gcgcTGGTTGGCGGCATCCTGCCACAGTGCGGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcaggg                                                                                                                                     >  1:33356/1‑96 (MQ=255)
                                                               gcgcTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcaggg                                                                                                                                     <  2:33356/100‑5 (MQ=255)
                                                               gcgcTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGa                                                                                    >  8:259800/1‑149 (MQ=255)
                                                                     gTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCg                                                                              >  1:46598/1‑149 (MQ=255)
                                                                        ggcggcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGt                                                                           >  7:269929/1‑149 (MQ=255)
                                                                        ggcggcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATCGGATGAGGCGCAGCGGTTGACTGCCggg                                                                            <  1:209904/148‑1 (MQ=255)
                                                                         gcggcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGt                                                                           <  3:66761/148‑1 (MQ=255)
                                                                            gcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTcaa                                                                       >  5:143071/1‑149 (MQ=255)
                                                                            gcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTcaa                                                                       >  4:311310/1‑149 (MQ=255)
                                                                             cATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTcaac                                                                      <  4:322052/149‑1 (MQ=255)
                                                                                    ccACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCgg                                                               <  4:294474/149‑1 (MQ=255)
                                                                                    ccACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGGGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCgg                                                               >  6:231001/1‑149 (MQ=255)
                                                                                      acaGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGCCTGCCGGGTTCAACAAGCGGGtt                                                             >  8:214921/1‑149 (MQ=255)
                                                                                      acaGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGtt                                                             <  4:7382/149‑1 (MQ=255)
                                                                                       caGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGttt                                                            <  8:269929/149‑1 (MQ=255)
                                                                                         gTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                          <  1:232347/149‑1 (MQ=255)
                                                                                         gTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                          <  6:135816/149‑1 (MQ=255)
                                                                                         gTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                          <  7:100517/149‑1 (MQ=255)
                                                                                                       gcgctggcTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGt                                            <  8:302156/149‑1 (MQ=255)
                                                                                                         gctggctgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAg                                          >  4:244406/1‑149 (MQ=255)
                                                                                                             gctgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGcc                                      >  6:114335/1‑149 (MQ=255)
                                                                                                               tgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGcc                                      >  2:22067/1‑147 (MQ=255)
                                                                                                               tgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGcc                                      >  3:170080/1‑147 (MQ=255)
                                                                                                                 aGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGa                                    <  6:143071/147‑1 (MQ=255)
                                                                                                                      aTGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAgcg                             >  3:170031/1‑149 (MQ=255)
                                                                                                                               gACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGc                    <  4:118894/149‑1 (MQ=255)
                                                                                                                                     gacACCCTGTCTCAAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGTATGATGCGCAGGGGTTGACTGCCTGGTTCAACAAGCCGGTTTACATGCTGGGTGGGGTAGGGCCGAGTTAGCGCGAGCAGGCCTtgg               <  2:290627/146‑1 (MQ=255)
                                                                                                                                     gTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                          >  1:368563/1‑105 (MQ=255)
                                                                                                                                     gTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                          <  2:368563/105‑1 (MQ=255)
                                                                                                                                         cccTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGcc          >  3:104710/1‑149 (MQ=255)
                                                                                                                                           cTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCgg        >  5:340467/1‑149 (MQ=255)
                                                                                                                                           cTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCgg        <  5:231001/149‑1 (MQ=255)
                                                                                                                                            tGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCggg       >  4:232890/1‑149 (MQ=255)
                                                                                                                                             gTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCgggg      <  7:259800/149‑1 (MQ=255)
                                                                                                                                                 ccAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCGGGGcaca  <  3:244406/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGGCACCTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCGGGGCACA  >  NC_002947/1537605‑1537898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑