Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,537,757 A→T 6.8% Q255L (CAG→CTG)  PP_1348 → MutT/nudix family protein/thiamine‑phosphate pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,537,7570AT 6.8% 221.6 / 4.8 73Q255L (CAG→CTG) PP_1348MutT/nudix family protein/thiamine‑phosphate pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base A (31/37);  new base T (2/3);  total (33/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

ACCTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCGGGGCACAGGGTG  >  NC_002947/1537610‑1537903
                                                                                                                                                   |                                                                                                                                                  
aCCTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAg                                                                                                                                                   <  4:87706/149‑1 (MQ=255)
 ccTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAgg                                                                                                                                                  <  1:286612/149‑1 (MQ=255)
  cTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGCGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGc                                                                                                                                                 >  3:294474/1‑149 (MQ=255)
    gACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGa                                                                                                                                               >  4:6066/1‑149 (MQ=255)
      cGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGAcc                                                                                                                                             <  6:378185/149‑1 (MQ=255)
       ggCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcag                                                                                                                                            <  6:350502/149‑3 (MQ=255)
            cgcAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACt                                                                                                                                       <  1:96023/149‑1 (MQ=255)
               aGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCAc                                                                                                                                    >  5:135816/1‑149 (MQ=255)
               aGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCAc                                                                                                                                    >  7:2731/1‑149 (MQ=255)
                       aaGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGc                                                                                                                            >  2:227330/1‑149 (MQ=255)
                       aaGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGc                                                                                                                            >  7:302156/1‑149 (MQ=255)
                        aGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCg                                                                                                                           <  4:56052/149‑1 (MQ=255)
                          tACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcagg                                                                                                                                           >  1:41254/1‑128 (MQ=255)
                          tACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcagg                                                                                                                                           <  2:41254/131‑4 (MQ=255)
                            cgccgcCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTAACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGTCGGTGc                                                                                                                       >  2:126765/1‑149 (MQ=255)
                             gccgccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGcc                                                                                                                      >  6:256239/1‑149 (MQ=255)
                               cgccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGt                                                                                                                    <  1:227330/149‑1 (MQ=255)
                                gccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGCCTCACCCAGAGGCGGTGCCGtt                                                                                                                   >  8:58025/1‑149 (MQ=255)
                                gccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGtt                                                                                                                   <  6:347855/149‑1 (MQ=255)
                                gccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGtt                                                                                                                   <  8:306784/149‑1 (MQ=255)
                                 ccAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtg                                                                                                                  <  7:127848/149‑1 (MQ=255)
                                    ggggCCGACCGTTCCCCAAGGAGGGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGTGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtggta                                                                                                               <  7:102012/149‑3 (MQ=255)
                                    ggggCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtggga                                                                                                               <  7:102010/149‑1 (MQ=255)
                                       gCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatgg                                                                                                            <  5:256239/149‑1 (MQ=255)
                                        ccGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggg                                                                                                           <  3:6066/149‑1 (MQ=255)
                                        ccGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggg                                                                                                           <  8:47654/149‑1 (MQ=255)
                                         cGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggga                                                                                                          <  4:363065/149‑1 (MQ=255)
                                         cGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtgggatggga                                                                                                          <  8:117999/149‑1 (MQ=255)
                                          gACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTgggatgggat                                                                                                         <  6:132782/149‑1 (MQ=255)
                                             cGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAg                                                                                                      <  7:298640/149‑1 (MQ=255)
                                              gTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAgg                                                                                                     >  3:118894/1‑149 (MQ=255)
                                                  cccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtg                                                                                                                  <  3:340153/132‑1 (MQ=255)
                                                  cccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTtg                                                                                                                  >  4:340153/1‑132 (MQ=255)
                                                   ccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAg                                                                                                >  8:100517/1‑149 (MQ=255)
                                                   ccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAg                                                                                                <  4:326861/149‑1 (MQ=255)
                                                   ccAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAg                                                                                                >  4:66761/1‑149 (MQ=255)
                                                    cAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGc                                                                                               >  3:322052/1‑149 (MQ=255)
                                                          gcgcTGGTTGGCGGCATCCTGCCACAGTGCGGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcaggg                                                                                                                                          >  1:33356/1‑96 (MQ=255)
                                                          gcgcTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCTGCACTGGCGAcaggg                                                                                                                                          <  2:33356/100‑5 (MQ=255)
                                                          gcgcTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGa                                                                                         >  8:259800/1‑149 (MQ=255)
                                                                gTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCg                                                                                   >  1:46598/1‑149 (MQ=255)
                                                                   ggcggcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGt                                                                                >  7:269929/1‑149 (MQ=255)
                                                                   ggcggcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATCGGATGAGGCGCAGCGGTTGACTGCCggg                                                                                 <  1:209904/148‑1 (MQ=255)
                                                                    gcggcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGt                                                                                <  3:66761/148‑1 (MQ=255)
                                                                       gcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTcaa                                                                            >  4:311310/1‑149 (MQ=255)
                                                                       gcATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTcaa                                                                            >  5:143071/1‑149 (MQ=255)
                                                                        cATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTcaac                                                                           <  4:322052/149‑1 (MQ=255)
                                                                               ccACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCgg                                                                    <  4:294474/149‑1 (MQ=255)
                                                                               ccACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGGGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCgg                                                                    >  6:231001/1‑149 (MQ=255)
                                                                                 acaGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGCCTGCCGGGTTCAACAAGCGGGtt                                                                  >  8:214921/1‑149 (MQ=255)
                                                                                 acaGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGtt                                                                  <  4:7382/149‑1 (MQ=255)
                                                                                  caGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGttt                                                                 <  8:269929/149‑1 (MQ=255)
                                                                                    gTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                               <  6:135816/149‑1 (MQ=255)
                                                                                    gTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                               <  7:100517/149‑1 (MQ=255)
                                                                                    gTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                               <  1:232347/149‑1 (MQ=255)
                                                                                                  gcgctggcTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGt                                                 <  8:302156/149‑1 (MQ=255)
                                                                                                    gctggctgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAg                                               >  4:244406/1‑149 (MQ=255)
                                                                                                        gctgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGcc                                           >  6:114335/1‑149 (MQ=255)
                                                                                                          tgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGcc                                           >  2:22067/1‑147 (MQ=255)
                                                                                                          tgAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGcc                                           >  3:170080/1‑147 (MQ=255)
                                                                                                            aGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGa                                         <  6:143071/147‑1 (MQ=255)
                                                                                                                 aTGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAgcg                                  >  3:170031/1‑149 (MQ=255)
                                                                                                                          gACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGc                         <  4:118894/149‑1 (MQ=255)
                                                                                                                                gacACCCTGTCTCAAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGTATGATGCGCAGGGGTTGACTGCCTGGTTCAACAAGCCGGTTTACATGCTGGGTGGGGTAGGGCCGAGTTAGCGCGAGCAGGCCTtgg                    <  2:290627/146‑1 (MQ=255)
                                                                                                                                gTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                               >  1:368563/1‑105 (MQ=255)
                                                                                                                                gTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTAc                                                               <  2:368563/105‑1 (MQ=255)
                                                                                                                                    cccTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGcc               >  3:104710/1‑149 (MQ=255)
                                                                                                                                      cTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCgg             >  5:340467/1‑149 (MQ=255)
                                                                                                                                      cTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCgg             <  5:231001/149‑1 (MQ=255)
                                                                                                                                       tGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCggg            >  4:232890/1‑149 (MQ=255)
                                                                                                                                        gTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCgggg           <  7:259800/149‑1 (MQ=255)
                                                                                                                                            ccAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCGGGGcaca       <  3:244406/149‑1 (MQ=255)
                                                                                                                                                tGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCC‑GGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCGGGGCACAGGgtg  >  7:76188/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCTGACGGCCGCGCAGCTGCGCAAGTACGCCGCCAGGGGCCGACCGTTCCCCAAGGAGCGCTGGTTGGCGGCATCCTGCCACAGTGCCGAAGAGCTGGCGCTGGCTGAGCAGATGGGCGTGGACTTTGTCACCCTGTCGCCAGTGCAGGCGACCCAGACTCACCCAGAGGCGGTGCCGTTGGGATGGGATGAGGCGCAGCGGTTGACTGCCGGGTTCAACAAGCCGGTTTACCTGCTGGGTGGGGTAGGGCCGAGTGAGCGCGAGCAGGCCTGGGAAGCCGGGGCACAGGGTG  >  NC_002947/1537610‑1537903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑