Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,547,033 T→A 9.3% W124R (TGG→AGG)  PP_1357 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,547,0330TA 9.3% 112.3 / 3.6 43W124R (TGG→AGG) PP_1357hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/22);  new base A (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

GGACGCCTACAGCGGAGAGAAAGCCAAGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGTACAACCGCGAGACCAAGGACGACG  >  NC_002947/1546885‑1547180
                                                                                                                                                    |                                                                                                                                                   
ggACGCCTACAGCGGAGAGAAAGCCAAGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCgtgt                                                                                                                                                     <  8:132039/149‑1 (MQ=255)
 gACGCCTACAGCGGAGAGAAAGCCAAGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGtgtg                                                                                                                                                    >  2:41223/1‑149 (MQ=255)
   cGCCTACAGCGGAGAGAAAGCCAAGTCGCTCAAGGCGGCAGAGGCAGGCAAGATGACCTTGGCCAATGGCGAATCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATTAAGCCCAAGCCGCTGGTCGAGGATTTCgtgttg                                                                                                                                                   >  8:297392/1‑146 (MQ=255)
      cTACAGCGGAGAGAAAGCCAAGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAg                                                                                                                                               <  8:102715/149‑1 (MQ=255)
                      gCCAAGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGgc                                                                                                                               >  1:84082/1‑149 (MQ=255)
                         aaGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCt                                                                                                                            <  5:99717/149‑1 (MQ=255)
                         aaGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCt                                                                                                                            <  5:174723/149‑1 (MQ=255)
                         aaGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCt                                                                                                                            <  4:184131/149‑1 (MQ=255)
                               cTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTa                                                                                                                      >  2:297955/1‑149 (MQ=255)
                                  aaGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTAc                                                                                                                     >  3:275713/1‑147 (MQ=255)
                                  aaGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACaa                                                                                                                   <  2:221534/149‑1 (MQ=255)
                                  aaGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACaa                                                                                                                   <  2:216045/149‑1 (MQ=255)
                                    ggcggcAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGAGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAgc                                                                                                                 >  3:364409/1‑149 (MQ=255)
                                    ggcggcAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGAGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAgc                                                                                                                 >  3:358524/1‑149 (MQ=255)
                                     gcggcAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAgcg                                                                                                                >  8:531/1‑149 (MQ=255)
                                       ggcAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCt                                                                                                                            <  2:360105/135‑1 (MQ=255)
                                       ggcAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCt                                                                                                                            >  1:360105/1‑135 (MQ=255)
                                         cAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGAGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGcc                                                                                                            <  4:364409/149‑1 (MQ=255)
                                         cAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGAGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGcc                                                                                                            <  4:358524/149‑1 (MQ=255)
                                          agagGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGcc                                                                                                            >  4:188118/1‑148 (MQ=255)
                                              gcaggcaAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGaa                                                                                                       >  7:96717/1‑149 (MQ=255)
                                               caggcaAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAAc                                                                                                      <  3:228870/149‑1 (MQ=255)
                                                aggcaAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACg                                                                                                     <  1:41223/149‑1 (MQ=255)
                                                   caAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGaca                                                                                                  <  1:59923/149‑1 (MQ=255)
                                                    aaGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGacac                                                                                                 >  5:144210/1‑149 (MQ=255)
                                                            cTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGAtt                                                                                         <  3:188118/149‑1 (MQ=255)
                                                            cTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGAt                                                                                          >  1:114271/1‑148 (MQ=255)
                                                                    aTGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTg                                                                                 <  7:531/149‑1 (MQ=255)
                                                                    aTGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTg                                                                                 <  4:369697/149‑1 (MQ=255)
                                                                        cGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCgag                                                                                                         <  7:223014/121‑1 (MQ=255)
                                                                        cGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCgag                                                                                                         >  8:223014/1‑121 (MQ=255)
                                                                              aCCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCaa                                                                         >  6:274403/1‑147 (MQ=255)
                                                                              aCCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCaa                                                                         <  5:274403/147‑1 (MQ=255)
                                                                              aCCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGa                                                                       <  5:208466/149‑1 (MQ=255)
                                                                                       ggTGGCGCTGGCCACCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACCAGCGCGGCGAGAACGACACCGACGGTTATGACGTGGGCTTCAAGCCCACTGccc                                                              >  8:160378/1‑149 (MQ=255)
                                                                                             gcTGGCCAGCATAGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGAAATCGTGTGGAAAGGTAATCTCGACCTGCCGCTGGACTACAAGCGCGCCGAGAACGACACAGACGATTAAGACGTGGGCTTCAAGACCACTGCCCGCCATg                                                        >  5:86090/1‑149 (MQ=255)
                                                                                                   cAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCt                                                  >  1:1862/1‑149 (MQ=255)
                                                                                                      cATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTggc                                               <  8:334844/149‑1 (MQ=255)
                                                                                                           aGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGca                                           >  4:175447/1‑148 (MQ=255)
                                                                                                                    tGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACcga                                                                                              >  4:268461/1‑88 (MQ=255)
                                                                                                                    tGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACcga                                                                                              <  3:268461/88‑1 (MQ=255)
                                                                                                                       aGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGt                              <  2:114271/149‑1 (MQ=255)
                                                                                                                           cAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGt                          <  8:96717/149‑1 (MQ=255)
                                                                                                                                 gCTGGTCGAGGACCTCCTGTGGAAAGGTAAGGCCTCCCTGGCCCCGGACAACAAGCGCGCCGCCAACCACACCGACGATTATGACGCTGGCTTCAAGTCCACTGCCCGCCATGGCCGCTCGCGGCACAACGCCGAGGGTGAGTACAccc                    <  4:275713/149‑1 (MQ=255)
                                                                                                                                      tCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGTACAACcgc                  >  7:232982/1‑146 (MQ=255)
                                                                                                                                            aTTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGTACAACCGCGAGACCAAg         <  7:297392/149‑1 (MQ=255)
                                                                                                                                                  tgtgGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGTACAACCGCGAGACCAAGgacgac   >  4:149752/1‑149 (MQ=255)
                                                                                                                                                  tgtgGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGTACAACCGCGAGACCAAGgacgac   <  3:249697/149‑1 (MQ=255)
                                                                                                                                                   gtgGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGTACAACCGCGAGACCAAGGacgacg  >  2:353832/1‑149 (MQ=255)
                                                                                                                                                    tgGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAAc                                       >  2:368779/1‑111 (MQ=255)
                                                                                                                                                    tgGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAAc                                       <  1:368779/111‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGACGCCTACAGCGGAGAGAAAGCCAAGTCGCTCAAGGCGGCAGAGGCAGGCAAGGTGACCTTGGCCAATGGCGATTCACCGAAGACGGTGGCGCTGGCCAGCATCGAGCAGATCATGAAGCCCAAGCCGCTGGTCGAGGATTTCGTGTGGAAAGGTAATGTCGACGTGGCGCTGGACTACAAGCGCGCCGAGAACGACACCGACGATTATGACGTGGGCTTCAAGACCACTGCCCGCCATGGCCGCTGGCGGCACAACGCCGAGGGTGAGTACAACCGCGAGACCAAGGACGACG  >  NC_002947/1546885‑1547180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑