Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,555,388 A→C 7.6% intergenic (+26/+48) sbcB → / ← PP_1366 exodeoxyribonuclease I/transcriptional regulator MvaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,555,3880AC 7.6% 145.2 / 2.4 50intergenic (+26/+48)sbcB/PP_1366exodeoxyribonuclease I/transcriptional regulator MvaT
Reads supporting (aligned to +/- strand):  ref base A (27/19);  new base C (2/2);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGGCGACTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTC  >  NC_002947/1555242‑1555528
                                                                                                                                                   |                                                                                                                                            
gggCGACTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCaa                                                                                                                                              <  6:84563/147‑1 (MQ=255)
     aCTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCg                                                                                                                                       <  6:287307/149‑1 (MQ=255)
      cTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTAATCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCgg                                                                                                                                      >  5:66203/1‑149 (MQ=255)
          gAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCAGGGCAACGGCAGGCTCGACAATTGCAGGACCGGTATACAATCGGCTGATGGAAGATAACCAAAAACCCCGCAAATGTGCGGGCGt                                                                                                                                   >  1:299422/1‑149 (MQ=255)
          gAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGtt                                                                                                                                  >  6:211082/1‑149 (MQ=255)
           aaaaGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGttt                                                                                                                                 >  4:263447/1‑149 (MQ=255)
            aaaGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGtttt                                                                                                                                <  6:151443/149‑1 (MQ=255)
              aGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTttg                                                                                                                              >  3:279113/1‑149 (MQ=255)
               ggCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTttgt                                                                                                                             <  3:263447/149‑1 (MQ=255)
                  gcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAACCCGCCGGCAATGTGCCGGCGTTTTtgttgt                                                                                                                          <  3:232372/149‑1 (MQ=255)
                                           gacgaGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGa                                                                                                  >  8:187677/1‑148 (MQ=255)
                                                ggcaggcaggcGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAAt                                                                                              >  5:356433/1‑147 (MQ=255)
                                                 gcaggcaggcGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAg                                                                                           >  4:333400/1‑149 (MQ=255)
                                                  caggcaggcGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGt                                                                                          <  7:99583/149‑1 (MQ=255)
                                                     gcaggcGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGttt                                                                                                                                 >  5:263120/1‑107 (MQ=255)
                                                     gcaggcGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGttt                                                                                                                                 <  6:263120/107‑1 (MQ=255)
                                                                ttGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGc                                                                             <  4:279113/148‑1 (MQ=255)
                                                                ttGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGAGAATCAAGAGGGCGTATGCGGATAATAAGTACAGCAGGGTTTcc                                                                            >  5:294510/1‑149 (MQ=255)
                                                                   gAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATc                                                                                             >  4:248520/1‑129 (MQ=255)
                                                                   gAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATc                                                                                             <  3:248520/129‑1 (MQ=255)
                                                                      gCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCtt                                                                      >  2:71587/1‑149 (MQ=255)
                                                                            gCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCa                                                                   <  5:13275/147‑1 (MQ=255)
                                                                            gCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAAc                                                                 >  3:156110/1‑149 (MQ=255)
                                                                              aaCTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCa                                                               >  8:2197/1‑149 (MQ=255)
                                                                                   cATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTc                                                           <  6:66203/148‑1 (MQ=255)
                                                                                    aTGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTcgc                                                         >  7:88471/1‑149 (MQ=255)
                                                                                            cAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCaa                                                 <  5:21332/149‑1 (MQ=255)
                                                                                              aTTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGt                                               <  7:9869/149‑1 (MQ=255)
                                                                                              aTTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTGTTTGTTGTGCGAAGCAAGAGGGCGGCTGCGGTGAATCAGTCCAGCAGGGTTGCCCCGCTTTCAACCACGTCGCCGCCCCAAGt                                               >  8:265143/1‑149 (MQ=255)
                                                                                                 gCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTgg                                             <  7:99173/148‑1 (MQ=255)
                                                                                                 gCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGc                                            >  7:323898/1‑149 (MQ=255)
                                                                                                   aGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCtt                                          >  6:116638/1‑149 (MQ=255)
                                                                                                     gAAAGCTCTTCAATCGGCTGATGGCAGATAAACAAACACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTc                                        <  4:31082/149‑1 (MQ=255)
                                                                                                      aaCGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTcc                                       <  8:324766/149‑1 (MQ=255)
                                                                                                         gCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCAc                                     >  7:221211/1‑148 (MQ=255)
                                                                                                             ttCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCtt                                 >  7:193875/1‑148 (MQ=255)
                                                                                                                agtCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAg                             >  6:369648/3‑149 (MQ=255)
                                                                                                                aaTCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGAAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAg                             >  6:168855/1‑149 (MQ=255)
                                                                                                                 aTCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCNCCGCCCCAAGTGGCTTTCCACTCTTTCAgg                            <  1:32087/149‑1 (MQ=255)
                                                                                                                     gCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACTTCGCCGCCCCAAGTGGCGTTCAACTCTTTCAGGGTCt                        >  4:256884/1‑149 (MQ=255)
                                                                                                                         aTGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtg                      <  3:333400/147‑1 (MQ=255)
                                                                                                                              aGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgc               <  5:369648/149‑1 (MQ=255)
                                                                                                                               gATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgcc              >  6:312188/1‑149 (MQ=255)
                                                                                                                                  aaaCAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgccgcc           >  5:44007/1‑149 (MQ=255)
                                                                                                                                    aCAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCCCGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGGCTTGTGGTTGCCGCCtt         >  3:63581/1‑149 (MQ=255)
                                                                                                                                    aCAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCtt         >  8:85865/1‑149 (MQ=255)
                                                                                                                                       aaaaCGCCGGCACATTGCCGGCGTCTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCAGCCTTTgg      >  7:47394/1‑149 (MQ=255)
                                                                                                                                         aaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGtt    <  8:323898/149‑1 (MQ=255)
                                                                                                                                          aCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGttt   >  8:177583/1‑149 (MQ=255)
                                                                                                                                           cGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTc  <  7:187677/149‑1 (MQ=255)
                                                                                                                                           cGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTc  <  2:299422/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GGGCGACTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCC‑TGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTC  >  NC_002947/1555242‑1555528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑