Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,579,674 G→C 72.6% Q575E (CAG→GAG)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,579,6740GC72.6% 80.7 / 32.5 51Q575E (CAG→GAG) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base G (8/6);  new base C (19/18);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGGCCTGCGAAGTTGAAACCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGGCGCCTACACTGCGCTCGTAG  >  NC_002947/1579536‑1579819
                                                                                                                                          |                                                                                                                                                 
cgcgGCCTGCGAAGTTGAAACCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAg                                                                                                                                          >  3:68783/1‑148 (MQ=255)
cgcgGCCTGCGAAGTTGAAACCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGc                                                                                                                                                <  1:361297/142‑1 (MQ=255)
cgcgGCCTGCGAAGTTGAAACCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGc                                                                                                                                                >  2:361297/1‑142 (MQ=255)
      cTGCGAAGTTGAAACCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGccc                                                                                                                                   <  4:68783/149‑1 (MQ=255)
              ttGAAACCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTCCGCCCTGGtcttc                                                                                                                           >  6:109247/1‑149 (MQ=255)
                  aaacGTTGATGGTGAACACCGAGGATAGGGTATCGGATTGGGCCTTGTGAAGGTATTGTCGCTTGTGGTCTACCACGACCTGCGTGCGTTCGGCACTGGAGCGGGCCGGGGTATGCTCGTCATCTAACTGTACGGCCTGGtgttcttgt                                                                                                                       <  2:72000/146‑3 (MQ=255)
                  aaCCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGtcttcttct                                                                                                                       <  6:332158/149‑1 (MQ=255)
                      gTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCa                                                                                                                   <  5:130674/149‑1 (MQ=255)
                                  cacCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAg                                                                                                       <  8:42285/149‑1 (MQ=255)
                                  cacCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAg                                                                                                       >  1:272705/1‑149 (MQ=255)
                                   acCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAgg                                                                                                      >  1:349085/1‑149 (MQ=255)
                                    ccGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAggg                                                                                                     <  1:15716/149‑1 (MQ=255)
                                    ccGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAggg                                                                                                     <  2:182709/149‑1 (MQ=255)
                                     cgacgaTACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTa                                                                                                        <  5:343717/145‑1 (MQ=255)
                                     cgacgaTACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTa                                                                                                        >  6:343717/1‑145 (MQ=255)
                                           tACGGTATCGGCGTCGTCCTTGAGCAGGTAGTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCggg                                                                                              <  8:339810/149‑1 (MQ=255)
                                              ggTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCAGGCg                                                                                            >  6:341494/1‑148 (MQ=255)
                                              ggTATCGGATTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCg                                                                                            >  1:230774/1‑148 (MQ=255)
                                               gTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCg                                                                                                <  7:79183/143‑1 (MQ=255)
                                               gTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCg                                                                                                >  8:79183/1‑143 (MQ=255)
                                                 aTCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGaa                                                                                          >  3:95275/1‑147 (MQ=255)
                                                  tCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAg                                                                                       >  7:56722/1‑149 (MQ=255)
                                                   cGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGc                                                                                      >  1:84683/1‑149 (MQ=255)
                                                           tCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCAt                                                                              >  2:25224/1‑149 (MQ=255)
                                                             cTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATg                                                                             <  4:95275/148‑1 (MQ=255)
                                                             cTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGc                                                                            >  7:322444/1‑149 (MQ=255)
                                                              ttGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGcc                                                                           >  1:2023/1‑149 (MQ=255)
                                                              ttGAGCAGGTATACACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGTAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGACCTGGTCTTCTTCTGGCAGGAACGCGCGAGGGATGCGGGCGAACAGCCAGATCATgac                                                                           >  7:309846/1‑147 (MQ=255)
                                                                 aGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGa                                                                         <  1:25224/148‑1 (MQ=255)
                                                                  gCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCAGGCGAACAGCCAGATCATGCCGAcc                                                                       <  5:341494/149‑1 (MQ=255)
                                                                     ggTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCAc                                                                     >  3:220415/1‑148 (MQ=255)
                                                                         ttCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATc                                                                 <  2:230774/148‑1 (MQ=255)
                                                                         ttCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGAt                                                                  <  8:322444/147‑1 (MQ=255)
                                                                            aCGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAgc                                                              <  4:220415/148‑1 (MQ=255)
                                                                                     gTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGc                                                      >  7:129653/1‑147 (MQ=255)
                                                                                      tCGACAACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACAACGATCAGCGCATAGGCcag                                                   >  2:311725/1‑149 (MQ=255)
                                                                                                 cTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCa                                        <  8:129653/149‑1 (MQ=255)
                                                                                                    cgtgcgtTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCAc                                       <  2:2023/147‑1 (MQ=255)
                                                                                                      tgcgtTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCttg                                   <  4:311515/149‑1 (MQ=255)
                                                                                                       gcgtTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAg                                                        <  1:140838/127‑1 (MQ=255)
                                                                                                       gcgtTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAg                                                        >  2:140838/1‑127 (MQ=255)
                                                                                                       gcgtTCGGCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCttgt                                  >  6:157743/1‑149 (MQ=255)
                                                                                                             ggCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGTCAGCAGGAATGGCATCTTGTTGCGCa                            <  8:56722/149‑1 (MQ=255)
                                                                                                             ggCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTgcgc                             >  1:41191/1‑148 (MQ=255)
                                                                                                              gCACTGGAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCaa                           >  2:46757/1‑149 (MQ=255)
                                                                                                                    gAGCCGGCCGGGGTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGgc                     >  4:72284/1‑149 (MQ=255)
                                                                                                                               ggTCTGCACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGGCGCCTACACTgc          >  3:181155/1‑149 (MQ=255)
                                                                                                                                   tGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCCGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGGCGCCTACACTGCGCTc      >  4:102591/1‑149 (MQ=255)
                                                                                                                                     cACCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGGCGCCTACACTGCGCTCGt    <  5:157743/149‑1 (MQ=255)
                                                                                                                                      aCCTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGGCGCCTACACTGCGCTCGTa   <  5:109247/149‑1 (MQ=255)
                                                                                                                                       ccTCAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGGCGCCTACACTGCGCTCGTAg  >  3:334184/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
CGCGGCCTGCGAAGTTGAAACCGTTGACCGTGAACACCGACGATACGGTATCGGCTTCGTCCTTGAGCAGGTATTCACGCATCTGGTCGACCACGACCTGCGTGCGTTCGGCACTGGAGCCGGCCGGGGTCTGCACCTGAGCGAACAGTACGCCCTGGTCTTCTTCTGGCAGGAACGCGGTAGGGATGCGGGCGAACAGCCAGATCATGCCGACCACGATCAGCGCATAGGCCAGCAGGAATGGCACCTTGTTGCGCAAGATGGCGCCTACACTGCGCTCGTAG  >  NC_002947/1579536‑1579819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑