Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,600,070 A→T 6.6% Y372N (TAC→AAC)  bglX ← beta‑D‑glucoside glucohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,600,0700AT 6.6% 177.3 / 2.8 61Y372N (TAC→AAC) bglXbeta‑D‑glucoside glucohydrolase
Reads supporting (aligned to +/- strand):  ref base A (23/34);  new base T (2/2);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

TCGATCGGAGCATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAG  >  NC_002947/1599925‑1600205
                                                                                                                                                 |                                                                                                                                       
tCGATCGGAGCATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGTGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCgtagta                                                                                                                                      <  1:245001/149‑1 (MQ=255)
 cGATCGGAGCATCGGCCAGCGGGCCGACCAAGGCAATGTTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCgtagta                                                                                                                                      <  5:119282/148‑1 (MQ=255)
  gATCGGAGCATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTagtagt                                                                                                                                    >  3:325872/1‑149 (MQ=255)
  gATCGGAGCATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTagtagt                                                                                                                                    >  3:320620/1‑149 (MQ=255)
    tCGGAGCATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTcc                                                                                                                                  >  3:169166/1‑149 (MQ=255)
      ggAGCATCGGCCAGCGGGCCGACCAAGGCAATGCTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCACCCCAACCAGGATCCGGCGTGCACCGTCCCGCGCAGATACGCGGTGCGGGCGGTCGTAGCCGAATTAGTCCtt                                                                                                                                >  8:148611/1‑149 (MQ=255)
          ggtCGGCCCGCTCGCCGATCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGa                                                                                                                            <  8:259575/147‑1 (MQ=255)
          cATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCgtagta                                                                                                                                      >  1:69244/1‑139 (MQ=255)
          cATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCgtagta                                                                                                                                      <  2:69244/139‑1 (MQ=255)
            tCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATc                                                                                                                          <  6:212003/149‑1 (MQ=255)
              ggCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGTGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCg                                                                                                                         <  1:275114/148‑1 (MQ=255)
               gCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGt                                                                                                                       >  7:294434/1‑149 (MQ=255)
                ccAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGtt                                                                                                                      >  3:138923/1‑149 (MQ=255)
                      ggCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGCTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGcc                                                                                                                <  1:263356/149‑1 (MQ=255)
                        ccGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCtt                                                                                                              <  6:268816/149‑1 (MQ=255)
                           aCCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGcc                                                                                                           >  5:364042/1‑149 (MQ=255)
                             cAAGGCAATGGTGCCGGCCCTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCgta                                                                                                                                         <  1:133060/117‑1 (MQ=255)
                             cAAGGCAATGGTGCCGGCCCTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCgta                                                                                                                                         >  2:133060/1‑117 (MQ=255)
                              aaGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGAt                                                                                                        >  5:311831/1‑149 (MQ=255)
                                      ggTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACg                                                                                                 >  3:274931/1‑148 (MQ=255)
                                       gTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGt                                                                                                <  4:320620/148‑1 (MQ=255)
                                       gTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGt                                                                                                <  4:325872/148‑1 (MQ=255)
                                        tGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTAc                                                                                              <  4:138923/149‑1 (MQ=255)
                                          ccGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACgg                                                                                            <  2:249942/149‑1 (MQ=255)
                                          ccGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACgg                                                                                            <  8:287398/149‑1 (MQ=255)
                                          ccGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACgg                                                                                            >  4:330768/1‑149 (MQ=255)
                                           cGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCg                                                                                                                   <  3:237695/125‑1 (MQ=255)
                                           cGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCg                                                                                                                   >  4:237695/1‑125 (MQ=255)
                                           cGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACggg                                                                                           >  8:106678/1‑149 (MQ=255)
                                           cGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACggg                                                                                           <  2:206552/149‑1 (MQ=255)
                                           cGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACggg                                                                                           >  5:237429/1‑149 (MQ=255)
                                               cTTTTTCAGCGGCAGGGTCTGGTTGCGGGTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGTTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCt                                                                                       <  8:348146/149‑1 (MQ=255)
                                                       gCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAgg                                                                               >  6:54653/1‑149 (MQ=255)
                                                        cGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTAc                                                                                              >  8:305199/1‑133 (MQ=255)
                                                        cGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTAc                                                                                              <  7:305199/133‑1 (MQ=255)
                                                        cGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGc                                                                              <  3:330768/149‑1 (MQ=255)
                                                         ggCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGcc                                                                             <  4:169166/149‑1 (MQ=255)
                                                          gCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGccc                                                                            <  4:319113/149‑1 (MQ=255)
                                                                      tGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTg                                                                <  7:126290/149‑1 (MQ=255)
                                                                        cGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACa                                                                                 >  4:104409/1‑130 (MQ=255)
                                                                        cGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACa                                                                                 <  3:104409/130‑1 (MQ=255)
                                                                          gTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCAc                                                            >  3:184748/1‑149 (MQ=255)
                                                                               ccAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTTGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAg                                                        >  6:53223/1‑148 (MQ=255)
                                                                               ccAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTTGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGc                                                       >  6:58773/1‑149 (MQ=255)
                                                                                cagcagCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCa                                                      >  1:91250/1‑149 (MQ=255)
                                                                                  gcagcaCCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACt                                                    >  4:98085/1‑149 (MQ=255)
                                                                                    agcaCCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTTCGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTc                                                  <  8:314729/149‑1 (MQ=255)
                                                                                     gcaCCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTcc                                                 <  6:177900/149‑2 (MQ=255)
                                                                                      caCCAGGCTGCGGCGTGCCACGGCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCAc                                                <  7:148611/149‑1 (MQ=255)
                                                                                         cAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTAGCGGTGCAGGCGGTCGTTTCCGTAGTAGTCATTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTAGAACTTGGAACCCCGCACTTCCCGCa                                             >  8:319322/1‑149 (MQ=255)
                                                                                               gcggcgTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCt                                       <  4:184748/149‑1 (MQ=255)
                                                                                                cggcgTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTg                                      <  6:237429/149‑1 (MQ=255)
                                                                                                cggcgTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTg                                      <  7:6369/149‑1 (MQ=255)
                                                                                                 ggcgTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTgg                                     <  5:54653/149‑1 (MQ=255)
                                                                                                       ccACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGc                               >  7:168600/1‑149 (MQ=255)
                                                                                                        cACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCt                              >  6:360221/1‑149 (MQ=255)
                                                                                                             cgcgcgCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCgcgc                         >  5:200617/1‑149 (MQ=255)
                                                                                                              gcgcgcAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCt                        <  8:168600/149‑1 (MQ=255)
                                                                                                               cgcgcAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTg                       <  7:106678/149‑1 (MQ=255)
                                                                                                                gcgcAGCTTCGCGGTGCAGGCGGTCGTTGCCGTTGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTgg                      <  5:53223/149‑1 (MQ=255)
                                                                                                                gcgcAGCTTCGCGGTGCAGGCGGTCGTTGCCGTTGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTgg                      <  5:58773/149‑1 (MQ=255)
                                                                                                                gcgcAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTgg                      <  6:364042/149‑1 (MQ=255)
                                                                                                                  gcAGCTTCGCGGTGCAGGAGGTCGTGGCGGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCAGCGACTGGTCCAGCTCGCGCTgg                      <  8:294434/147‑1 (MQ=255)
                                                                                                                    aGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCa                  >  2:89679/1‑149 (MQ=255)
                                                                                                                     gCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCAc                 <  4:274931/149‑1 (MQ=255)
                                                                                                                      cTTCGCGGTGGAGGCGGTCGTTGCGGTAGTAGTCCTTCTGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCAc                 <  2:91250/148‑1 (MQ=255)
                                                                                                                         cgcgGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTc              <  6:311831/148‑1 (MQ=255)
                                                                                                                             gTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCg          >  1:241101/1‑148 (MQ=255)
                                                                                                                                    cGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTcag  <  2:241101/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                       
TCGATCGGAGCATCGGCCAGCGGGCCGACCAAGGCAATGGTGCCGGCCTTTTTCAGCGGCAGGGTCTGGTTGCGGTTTTCCAGCAGCACCAGGCTGCGGCGTGCCACGTCGCGCGCAGCTTCGCGGTGCAGGCGGTCGTTGCCGTAGTAGTCCTTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAG  >  NC_002947/1599925‑1600205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑