| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,602,066 | T→A | 11.1% | intergenic (+79/‑88) | PP_1404 → / → PP_5469 | hypothetical protein/SAM‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,602,066 | 0 | T | A | 11.1% | 114.7 / 6.0 | 45 | intergenic (+79/‑88) | PP_1404/PP_5469 | hypothetical protein/SAM‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (20/20); new base A (3/2); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01 | |||||||||||
CTTAGCCAGCACGTGCCGCCAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGCCGGATGCGCGCGCTCAGTTTCTCGA > NC_002947/1601919‑1602209 | ctTAGCCAGCACGTGCCGCCAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCtt > 5:167465/1‑149 (MQ=255) ccAGCACGTGCCGCCAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCg > 7:113711/1‑149 (MQ=255) cAGTACGTGCCGCCAACCCATGCACGCAAGGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACCGGCGACAGGGTGTCTGGCACCGGCTTTGCCgg > 5:25257/1‑149 (MQ=255) cACGTGCCGCCAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGACACCGGCAAAGCCGgtgc < 6:204542/149‑2 (MQ=255) tGCCGCCAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTcgc > 3:119977/1‑149 (MQ=255) tGCCGCCAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTcgc < 6:52196/149‑1 (MQ=255) cgccAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTCGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCggg < 5:328396/149‑1 (MQ=255) cAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCggg < 1:12628/146‑1 (MQ=255) cAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCggg > 2:12628/1‑146 (MQ=255) ccATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGccc > 3:307943/1‑149 (MQ=255) aaCGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAg < 3:249555/149‑1 (MQ=255) cccAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTg < 6:321895/148‑1 (MQ=255) ccAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTggg > 6:275465/1‑149 (MQ=255) ccAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTggg > 6:270289/1‑149 (MQ=255) ccAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCAAAGCCGgtgc > 7:108908/1‑114 (MQ=255) ccAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCAAAGCCGgtgc < 8:108908/115‑2 (MQ=255) cAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTgggg < 6:181005/149‑1 (MQ=255) aaCCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTgggg > 2:227611/1‑148 (MQ=255) ccGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCAc < 4:307943/149‑1 (MQ=255) ggTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTa < 4:119977/147‑1 (MQ=255) aTTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAg < 6:205536/149‑1 (MQ=255) tcGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGt > 8:292316/1‑149 (MQ=255) cTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCg > 8:159487/1‑149 (MQ=255) aaGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCtt < 8:246836/149‑1 (MQ=255) aaGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTACCGCtt > 8:167193/1‑149 (MQ=255) aGCGTCCAGCCTTGCTGGATTTCCTGTGGACACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTccc > 1:27022/1‑148 (MQ=255) cGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAg < 7:349892/149‑1 (MQ=255) gTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAgg < 8:216487/149‑1 (MQ=255) tCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGc > 6:14380/1‑149 (MQ=255) aGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCGTTGTAAACAGGGGACAGGGTGTCTGGAACCGGCTTTGCCGGTGTTCGCGGGAAAGCCCGATCCCAAAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGcccc < 6:167465/149‑1 (MQ=38) ccTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCt > 6:66341/1‑149 (MQ=255) ttGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCttt > 2:177209/1‑149 (MQ=255) cTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGGCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCttttt < 3:212999/148‑1 (MQ=255) cTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCttttt < 7:159487/148‑1 (MQ=255) gATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATg > 4:80162/1‑149 (MQ=255) gATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATg > 1:26721/1‑149 (MQ=255) gtgGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAg > 4:186388/1‑149 (MQ=255) ggCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTAc < 8:113711/149‑1 (MQ=255) caGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCt < 6:311636/149‑1 (MQ=255) aaaCAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGcc > 1:23152/1‑149 (MQ=255) aCAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGCCGGATgcgcg < 1:227611/149‑1 (MQ=255) gaacACCGGCAAAGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGCCGGATGCGCGCGCTCAGttt > 4:261559/4‑149 (MQ=255) aacACCGGCAAAGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTGCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGACGGCTGAGCGCGCTCAGTTtc > 8:121138/3‑149 (MQ=38) gCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGCCGGATGCGCGCGCTCAGTTtc < 3:186388/148‑1 (MQ=255) cACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATACGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGCCGGATGCGCGCGCTCAGTTtct < 6:25257/148‑1 (MQ=255) ccGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGCCGGATGCGCGCGCTCAGTTTCTCGa > 4:162343/1‑149 (MQ=255) | CTTAGCCAGCACGTGCCGCCAACCCATGCACGCAACGAGTTGCCCAACCGCTTGGTGATTCTCGACTGAAGGAGCGTCCAGCCTTGCTGGATTTCCTGTGGGCACAGGCTTGCCTTGGAAACAGGCGACAGGGTGTCTGGCACCGGCTTTGCCGGTGTTCGCGGGCAAGCCCGCTCCCACAGGGTGCTTGGGGCACGTTAGAATAGGCGTTCCGCTTCCCAAGGCCCCCTTTTTCATGTCCGCCAGTACTTCTGCCTCTTGCGCGCCGGATGCGCGCGCTCAGTTTCTCGA > NC_002947/1601919‑1602209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |