Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,622,190 G→C 6.9% E368D (GAG→GAC) ‡ PP_1421 → sensor histidine kinase TctE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,622,1900GC 6.9% 223.5 / 5.5 72E368D (GAG→GAC) ‡PP_1421sensor histidine kinase TctE
Reads supporting (aligned to +/- strand):  ref base G (34/33);  new base C (2/3);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

AGGCGGTGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACGGGTGTTCGAGCG  >  NC_002947/1622043‑1622336
                                                                                                                                                    |                                                                                                                                                  
aGGCGGTGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAgt                                                                                                                                                   <  6:218078/149‑1 (MQ=255)
  gCGGTGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAgttg                                                                                                                                                 >  6:79647/1‑149 (MQ=255)
    ggTGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAgttgtt                                                                                                                                               <  5:79647/149‑1 (MQ=255)
    ggTGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAgttgtt                                                                                                                                               <  6:223188/149‑1 (MQ=255)
     gTGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGttgttg                                                                                                                                              <  7:96746/149‑1 (MQ=255)
      tGCGCAGGGTCTGGACTTGAGTCTGTTTGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGa                                                                                                                                             <  2:269107/149‑1 (MQ=255)
      tGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGa                                                                                                                                             <  6:19552/149‑1 (MQ=255)
        cgcAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGGGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACAACTCGTTGAg                                                                                                                                            <  4:155338/148‑1 (MQ=255)
           aGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAAc                                                                                                                                        >  4:75451/1‑149 (MQ=255)
                cTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCtggt                                                                                                                                   >  2:358017/1‑149 (MQ=255)
                 tGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCtggtg                                                                                                                                  >  6:27253/1‑149 (MQ=255)
                   gACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCtggt                                                                                                                                   <  3:214448/146‑1 (MQ=255)
                   gACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGa                                                                                                                                <  2:368533/149‑1 (MQ=255)
                   gACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGa                                                                                                                                >  3:146029/1‑149 (MQ=255)
                     cTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGc                                                                                                                                           <  6:273891/136‑1 (MQ=255)
                     cTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGc                                                                                                                                           >  5:273891/1‑136 (MQ=255)
                      ttGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACaa                                                                                                                             >  4:83600/1‑149 (MQ=255)
                        gAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAAt                                                                                                                            <  1:67239/148‑1 (MQ=255)
                        gAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACAACTCGTTGAGCa                                                                                                                                          <  1:221494/134‑1 (MQ=255)
                        gAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACAACTCGTTGAGCa                                                                                                                                          >  2:221494/1‑134 (MQ=255)
                          gTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACaa                                                                                                                             >  2:56815/1‑145 (MQ=255)
                          gTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACaa                                                                                                                             <  1:56815/145‑1 (MQ=255)
                             aGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTg                                                                                                                      <  3:331186/149‑1 (MQ=255)
                              gTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACAACTCGTTGAGCaatg                                                                                                                                       <  3:179326/131‑3 (MQ=255)
                                    ccGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATa                                                                                                               <  4:325776/149‑1 (MQ=255)
                                     cgcgAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATAc                                                                                                              >  3:23735/1‑149 (MQ=255)
                                      gcgAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACg                                                                                                             >  1:104561/1‑149 (MQ=255)
                                       cgAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGc                                                                                                            <  5:27253/149‑1 (MQ=255)
                                         aGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCa                                                                                                          <  2:330300/149‑1 (MQ=255)
                                              ggCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCgg                                                                                                      <  3:351449/148‑1 (MQ=255)
                                               gCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GCGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGt                                                                                                    >  6:254158/1‑149 (MQ=255)
                                               gCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGt                                                                                                    >  6:314653/1‑149 (MQ=255)
                                                catggccatggcGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTg                                                                                                   >  6:255507/1‑149 (MQ=255)
                                                 atggccatggcGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTgg                                                                                                  <  3:83600/149‑1 (MQ=255)
                                                 atggccatggcGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTgg                                                                                                  <  2:104561/149‑1 (MQ=255)
                                                       atggcGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAAtgt                                                                                            <  8:33511/149‑1 (MQ=255)
                                                            gcCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGAttt                                                                                       <  8:184164/149‑1 (MQ=255)
                                                              cTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGCGGCC‑GAGTCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCACCGAGTTGTGGCGCAACCTGGTGGCCAATGACGTGGCGCATACGCAAGCGGGGGGCAATGTGAttttt                                                                                     >  7:351272/1‑148 (MQ=255)
                                                              cTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTg                                                                                     <  4:313675/149‑1 (MQ=255)
                                                              cTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTg                                                                                     >  2:129043/1‑149 (MQ=255)
                                                               tttGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAAGGTGATTTTGc                                                                                    >  5:220713/1‑149 (MQ=255)
                                                                   gcgcACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAAt                                                                                              <  4:265418/135‑1 (MQ=255)
                                                                   gcgcACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAAt                                                                                              >  3:265418/1‑135 (MQ=255)
                                                                     gcACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTaa                                                                              >  6:177692/1‑149 (MQ=255)
                                                                      cacaAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAAt                                                                             <  4:146029/149‑1 (MQ=255)
                                                                      cacaAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAAt                                                                             >  4:91531/1‑149 (MQ=255)
                                                                        caAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATgg                                                                           <  8:240791/149‑1 (MQ=255)
                                                                           gcgcGGGGTGGCGCTGGCGCTGGAGGCCGGAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCTTACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGgcg                                                                         >  1:22435/1‑149 (MQ=255)
                                                                                   tggcgctggcgcTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTg                                                                <  3:75451/149‑1 (MQ=255)
                                                                                       gctggcgctggAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCtgg                                                            <  3:220755/149‑1 (MQ=255)
                                                                                        ctggcgctggAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCtgga                                                           <  8:8937/149‑1 (MQ=255)
                                                                                           gcgctggAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCtggaagt                                                        <  2:182070/149‑1 (MQ=255)
                                                                                            cgctggAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCtggaagtg                                                       <  4:23735/149‑1 (MQ=255)
                                                                                              ctggAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGTATAATACATTGCTCAACGAGTTGTTGAGCAACCTTGTGGACAATGCCTTTGAGCATACGCAAGAGGGTGGAAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCtggaagtgtt                                                     <  4:304494/149‑3 (MQ=255)
                                                                                                 gAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCtggaagtggaa                                                    <  3:91531/147‑1 (MQ=255)
                                                                                                 gAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAga                                                  <  8:206869/149‑1 (MQ=255)
                                                                                                 gAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAga                                                  >  2:78024/1‑149 (MQ=255)
                                                                                                    gCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACTAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAgacga                                               >  6:273647/1‑149 (MQ=255)
                                                                                                        gAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACgg                                            >  3:276417/1‑149 (MQ=255)
                                                                                                        gAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACgg                                            <  5:254158/149‑1 (MQ=255)
                                                                                                               cGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAgg                                      >  7:134959/1‑148 (MQ=255)
                                                                                                                ggTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTa                                    >  6:364160/1‑149 (MQ=255)
                                                                                                                                gAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAgcg                    >  6:82628/1‑149 (MQ=255)
                                                                                                                                 aGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAgcgc                   >  6:284140/1‑149 (MQ=255)
                                                                                                                                  gCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAgcgc                   >  2:130479/1‑148 (MQ=255)
                                                                                                                                     gACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCGGTGGCCGAGCGCGAAc               >  7:128358/1‑149 (MQ=255)
                                                                                                                                       cATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACgg             <  5:364160/149‑1 (MQ=255)
                                                                                                                                       cATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTCGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACgg             >  3:260558/1‑149 (MQ=255)
                                                                                                                                         ttGCTCAACAACTCGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACGGgt           >  6:335602/1‑149 (MQ=255)
                                                                                                                                           gCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACGGgtgt         >  7:108137/1‑149 (MQ=255)
                                                                                                                                               aaCGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGt                                                                                                    >  4:355538/1‑54 (MQ=255)
                                                                                                                                               aaCGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGt                                                                                                    <  3:355538/54‑1 (MQ=255)
                                                                                                                                               aaCGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGACCGGGTGTTc       >  6:328703/1‑147 (MQ=255)
                                                                                                                                               aaCGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACGGGTGTTc       >  3:306842/1‑147 (MQ=255)
                                                                                                                                               aaCGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACGGGTGTTc       <  1:358017/147‑1 (MQ=255)
                                                                                                                                                  gAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACGGGTGTTCGAGCg  >  8:192177/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGGCGGTGCGCAGCGTCTGGACTTGAGTCAGTTGGCCCGCGAGCTGGGCATGGCCATGGCGCCTTTGGCGCACAAGCGCGGGGTGGCGCTGGCGCTGGAGGCC‑GAGGCGCCGGTGTGGTTGAAGGGGGAGCCGACATTGCTCAACGAGTTGTTGAGCAACCTGGTGGACAATGCCTTGGCGCATACGCCAGCGGGTGGCAATGTGATTTTGCGGGTAATGGCGCCGGCAGTGCTGGAAGTGGAAGACGACGGGCCAGGTATTCCCGTGGCCGAGCGCGAACGGGTGTTCGAGCG  >  NC_002947/1622043‑1622336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑