Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,631,094 T→G 5.9% intergenic (+28/‑153) PP_1430 → / → lepA DegP‑like serine endoprotease/elongation factor 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,631,0940TG 5.9% 218.3 / 2.5 68intergenic (+28/‑153)PP_1430/lepADegP‑like serine endoprotease/elongation factor 4
Reads supporting (aligned to +/- strand):  ref base T (34/30);  new base G (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGT  >  NC_002947/1630951‑1631238
                                                                                                                                               |                                                                                                                                                
gTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCt                                                                                                                                             >  3:200409/1‑149 (MQ=255)
 ttCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTg                                                                                                                                            <  2:247645/149‑1 (MQ=255)
       cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttt                                                                                                                                      <  3:58620/149‑1 (MQ=255)
       cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCttt                                                                                                                                      >  6:383960/1‑149 (MQ=255)
       cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTAATAACTTTCAAGCTTGGTGAATAAGCAGTTTGGCAAGGCGAAAGGGCAGCTTCGGCTGCCGttt                                                                                                                                      >  2:335728/1‑149 (MQ=255)
        aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCtttt                                                                                                                                     <  6:105383/149‑1 (MQ=255)
        aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCtttt                                                                                                                                     >  8:188708/1‑149 (MQ=255)
        aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCtttt                                                                                                                                     >  8:284101/1‑149 (MQ=255)
        aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCtttt                                                                                                                                     <  3:168485/149‑1 (MQ=255)
           gAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCa                                                                                                                                  >  7:306286/1‑149 (MQ=255)
           gAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCtttcgcc                                                                                                                                  <  5:383960/149‑5 (MQ=255)
            aaTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCAt                                                                                                                                 >  6:248932/1‑149 (MQ=255)
            aaTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCAt                                                                                                                                 >  1:338705/1‑149 (MQ=255)
                cACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGaa                                                                                                                              >  8:80960/1‑148 (MQ=255)
                 aCCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATc                                                                                                                            >  6:279431/1‑149 (MQ=255)
                           ccAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTg                                                                                                                  >  2:271239/1‑149 (MQ=255)
                            cAAGGAACTGCCGAAGAATCGCTCCGTCTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGc                                                                                                                 >  6:165661/1‑149 (MQ=255)
                             aaGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGcc                                                                                                                >  2:5683/1‑149 (MQ=255)
                               ggAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCg                                                                                                               >  8:88528/1‑148 (MQ=255)
                                 aaCTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGaa                                                                                                            <  7:284101/149‑1 (MQ=255)
                                    tGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGa                                                                                                         >  5:382277/1‑149 (MQ=255)
                                      ccGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGAtt                                                                                                       >  2:333903/1‑149 (MQ=255)
                                       cGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTg                                                                                                      >  2:350003/1‑149 (MQ=255)
                                       cGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTg                                                                                                      <  7:153631/149‑1 (MQ=255)
                                       cGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCtttcg                                                                                                                                    >  8:291947/1‑117 (MQ=255)
                                       cGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCtttcg                                                                                                                                    <  7:291947/119‑3 (MQ=255)
                                        gaagaaTCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGc                                                                                                     >  2:48899/1‑149 (MQ=255)
                                              tCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTa                                                                                               <  1:271239/149‑1 (MQ=255)
                                                 ttCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGcc                                                                                                                <  2:115568/129‑1 (MQ=255)
                                                 ttCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGcc                                                                                                                >  1:115568/1‑129 (MQ=255)
                                                        tCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACa                                                                                     <  6:197885/149‑1 (MQ=255)
                                                           aTGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACagag                                                                                  >  3:266897/1‑149 (MQ=255)
                                                              cgcgTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGa                                                                                <  1:48899/148‑1 (MQ=255)
                                                              cgcgTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGa                                                                                <  4:200409/148‑1 (MQ=255)
                                                              cgcgTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGa                                                                                <  7:328479/148‑1 (MQ=255)
                                                                cgTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAAtc                                                                             <  5:248932/149‑1 (MQ=255)
                                                                   aCTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAAtctc                                                                           <  8:306286/148‑1 (MQ=255)
                                                                           aGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAAcc                                                                   <  7:188708/148‑1 (MQ=255)
                                                                               ccGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCcagca                                                              <  7:80960/149‑1 (MQ=255)
                                                                                      aGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                       <  7:88528/149‑1 (MQ=255)
                                                                                      aGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                       >  8:97632/1‑149 (MQ=255)
                                                                                      aGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                       <  1:5683/149‑1 (MQ=255)
                                                                                      aGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                       <  3:231354/149‑1 (MQ=255)
                                                                                        cTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCa                                                                        >  7:153695/1‑130 (MQ=255)
                                                                                        cTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCa                                                                        <  8:153695/130‑1 (MQ=255)
                                                                                         ttCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTAGAGTCGGACAGAGGAATCTCCCATAAACCAGCAGCTAATGGTa                                                    >  8:100635/1‑149 (MQ=255)
                                                                                           catcaCTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTa                                                    <  6:382277/147‑1 (MQ=255)
                                                                                            atcaCTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCCTACACCAGCAGCGTAAGGTACaa                                                 >  4:90041/1‑149 (MQ=255)
                                                                                             tcaCTTTCAAGCATGCTGAATAAGCAGTTTCGCAAGGCGAAAGGCCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGAAAGAGGAATCTCCCATAACACAGCAGCTTAAGGTACAAt                                                >  8:148573/1‑149 (MQ=255)
                                                                                                cTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTcc                                             >  7:4070/1‑149 (MQ=255)
                                                                                                 tttCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCg                                                                                                               <  8:328525/82‑1 (MQ=255)
                                                                                                 tttCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCg                                                                                                               >  7:328525/1‑82 (MQ=255)
                                                                                                 tttCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTccc                                            <  5:165661/149‑1 (MQ=255)
                                                                                                              gAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGACCTTTTTCATGAATCTTCACAATTGCCGGAATGATAGCTGTGTACAGTCGCACACAGGAATGTCCCAAAACCCACCCCAGTAACGTACAATTCCCGGCTATTTTTagg                               >  7:54324/1‑149 (MQ=255)
                                                                                                                aTAAGCAGTTTCGCAAGGCGAAAGGGCTGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGGAGCTTAAGGTACAATTCCCGGCTATTTTTcggcg                             <  1:349693/149‑1 (MQ=255)
                                                                                                                  aaGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTATCAAGAATCTTCACAATTGCCGGAATGATTGCTGAGTACAGTCGGACAGACGAATCTCCCATAACCCAGCAGCTTAAGGAACAAATGGGGGCTATTTTTCgggggg                           >  6:133999/1‑149 (MQ=255)
                                                                                                                  aaGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGACCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTGACGGCTATTTTTcggggtg                           >  6:139945/1‑145 (MQ=255)
                                                                                                                    gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcggcgg                            >  7:91319/1‑146 (MQ=255)
                                                                                                                    gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcggcgg                            <  8:91319/146‑1 (MQ=255)
                                                                                                                    gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCggcgggcg                         >  3:76798/1‑149 (MQ=255)
                                                                                                                     cAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCGTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGt                        >  1:25474/1‑149 (MQ=255)
                                                                                                                            gCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTc                 <  1:333903/149‑1 (MQ=255)
                                                                                                                             cAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCg                >  1:16995/1‑149 (MQ=255)
                                                                                                                               aGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCa              <  3:90041/149‑1 (MQ=255)
                                                                                                                                      aGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCtttt       >  3:211006/1‑149 (MQ=255)
                                                                                                                                      aGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCtttt       <  4:266897/149‑1 (MQ=255)
                                                                                                                                          cAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAg   <  2:16995/149‑1 (MQ=255)
                                                                                                                                           aGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGATGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAgt  <  5:279431/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGT  >  NC_002947/1630951‑1631238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑