Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,633,552 C→G 11.9% G167G (GGC→GGG)  lepB → signal peptidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,633,5520CG11.9% 164.8 / 10.9 58G167G (GGC→GGG) lepBsignal peptidase I
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base G (4/3);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGT  >  NC_002947/1633406‑1633698
                                                                                                                                                  |                                                                                                                                                  
gTCATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGc                                                                                                                                                    >  8:164233/1‑147 (MQ=255)
   aTCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGcc                                                                                                                                               >  2:57523/1‑149 (MQ=255)
       aGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGtt                                                                                                                                           <  1:9189/149‑1 (MQ=255)
        ggTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTg                                                                                                                                          >  8:149647/1‑149 (MQ=255)
            ggTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCg                                                                                                                                       >  7:44913/1‑148 (MQ=255)
                aCCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaacag                                                                                                                                  <  3:87969/149‑2 (MQ=255)
                    gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtgg                                                                                                                              >  5:155330/1‑149 (MQ=255)
                    gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCCGTTCGGCAACGGGCTGGCCGTTGACGaaca                                                                                                                                   <  2:363769/144‑1 (MQ=255)
                    gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCCGTTCGGCAACGGGCTGGCCGTTGACGaaca                                                                                                                                   >  1:363769/1‑144 (MQ=255)
                     cAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggt                                                                                                                             <  4:86271/149‑1 (MQ=255)
                       aCGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggtgg                                                                                                                           <  3:264295/149‑1 (MQ=255)
                          cgGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCa                                                                                                                        <  1:57523/149‑1 (MQ=255)
                             ggATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCg                                                                                                                     <  7:164233/149‑1 (MQ=255)
                                        tgtTCCGCTACCCGAGTCACCCGAATGTCACCTACCTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCAACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTAcc                                                                                                          <  8:7018/149‑1 (MQ=255)
                                          ttgcGCTACCCGTGTGACCCGATTGTCTACTAGATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTAccc                                                                                                         <  2:368937/145‑1 (MQ=255)
                                          ttCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCt                                                                                                        >  1:91208/1‑149 (MQ=255)
                                            ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTgg                                                                                                      >  5:369468/1‑149 (MQ=255)
                                            ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                       <  4:199143/148‑1 (MQ=255)
                                             cGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGCGGGCAGCGAGCCGGGTACCCTggg                                                                                                     <  8:314430/149‑1 (MQ=255)
                                              gCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                       >  2:339090/1‑146 (MQ=255)
                                              gCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                       <  1:339090/146‑1 (MQ=255)
                                                 aCCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGCCGTGGTCCGCTACACCAACGACAAGCGGCTCTGCGGCAACGGGCTGGCCGtt                                                                                                                                           >  4:341145/1‑107 (MQ=255)
                                                 aCCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGTTCCGCTACAGCTACGACATGCGGCTGTTCGGCAACGGGCTGGCCGtt                                                                                                                                           <  3:341145/107‑1 (MQ=255)
                                                  cccGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGt                                                                                                 <  5:238718/148‑1 (MQ=255)
                                                  cccGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTg                                                                                                <  6:23031/149‑1 (MQ=255)
                                                    cGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCt                                                                                              <  7:149647/149‑1 (MQ=255)
                                                     gAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTc                                                                                             >  8:246734/1‑149 (MQ=255)
                                                      aGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCa                                                                                            <  5:201903/149‑1 (MQ=255)
                                                          aCCCGAATGTCAACTACATCAAGCTTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAAGAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGcag                                                                                        >  8:299308/1‑147 (MQ=255)
                                                          aCCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGccc                                                                                                                                              <  6:360730/95‑1 (MQ=255)
                                                          aCCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGccc                                                                                                                                              >  5:360730/1‑95 (MQ=255)
                                                            ccGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGt                                                                                       >  6:299438/1‑148 (MQ=255)
                                                                  gTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGaa                                                                                                                                     >  7:160917/1‑96 (MQ=255)
                                                                  gTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGaa                                                                                                                                     <  8:160917/96‑1 (MQ=255)
                                                                   tCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGaa                                                                               <  6:369468/149‑1 (MQ=255)
                                                                      aCTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAg                                                                            >  3:118422/1‑149 (MQ=255)
                                                                           aTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCgg                                                                       <  5:299438/149‑1 (MQ=255)
                                                                             cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggtg                                                                                                                            <  4:24280/94‑1 (MQ=255)
                                                                             cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggtg                                                                                                                            >  3:24280/1‑94 (MQ=255)
                                                                                  gtgtGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGcc                                                                <  2:91208/149‑1 (MQ=255)
                                                                                       gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACATCTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAaca                                                           >  1:119237/1‑149 (MQ=255)
                                                                                          ggCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACAcc                                                         <  6:155330/148‑1 (MQ=255)
                                                                                           gCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCt                                                        <  4:118422/148‑1 (MQ=255)
                                                                                              tGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATc                                                    >  4:88200/1‑149 (MQ=255)
                                                                                              tGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATc                                                    >  2:357513/1‑149 (MQ=255)
                                                                                                             tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGAt                                                     >  1:180034/1‑133 (MQ=255)
                                                                                                             tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGAt                                                     <  2:180034/133‑1 (MQ=255)
                                                                                                              ccGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGcc                                    >  5:162705/1‑149 (MQ=255)
                                                                                                                                gCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGccgccg                   >  7:7208/1‑148 (MQ=255)
                                                                                                                                     tGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATTCCGCCGGACcag             >  7:74115/1‑149 (MQ=255)
                                                                                                                                      gTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcag             >  2:65444/1‑148 (MQ=255)
                                                                                                                                       ttCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcag             <  1:357513/147‑1 (MQ=255)
                                                                                                                                        tCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcagcag          >  7:89359/1‑149 (MQ=255)
                                                                                                                                          ggcAACGGGCTGGCCGTTGACGAACATCTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACAACTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACAAGCAGTg        >  8:283711/3‑149 (MQ=255)
                                                                                                                                          ggcAACGGGCTGGCCGTTGACGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg        >  6:166194/3‑149 (MQ=255)
                                                                                                                                          gTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg        <  8:7208/149‑1 (MQ=255)
                                                                                                                                               cGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAac                                                            <  7:273411/92‑1 (MQ=255)
                                                                                                                                               cGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAac                                                            >  8:273411/1‑92 (MQ=255)
                                                                                                                                                ggCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGAGCGGGGCGGCGGAACACGTGAGCCGCAGGGAAATGGGCCGTTTGCCCATGCCGCCGGACCAGCAGTGGACCGt  >  6:171682/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTCATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGT  >  NC_002947/1633406‑1633698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑