Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,633,563 C→A 11.1% A171D (GCC→GAC)  lepB → signal peptidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,633,5630CA11.1% 154.7 / 9.9 54A171D (GCC→GAC) lepBsignal peptidase I
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base A (3/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

GGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCGGGCCAT  >  NC_002947/1633418‑1633711
                                                                                                                                                 |                                                                                                                                                    
ggTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCg                                                                                                                                                    >  7:44913/1‑148 (MQ=255)
    aCCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaacag                                                                                                                                               <  3:87969/149‑2 (MQ=255)
        gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtgg                                                                                                                                           >  5:155330/1‑149 (MQ=255)
        gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCCGTTCGGCAACGGGCTGGCCGTTGACGaaca                                                                                                                                                >  1:363769/1‑144 (MQ=255)
        gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCCGTTCGGCAACGGGCTGGCCGTTGACGaaca                                                                                                                                                <  2:363769/144‑1 (MQ=255)
         cAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggt                                                                                                                                          <  4:86271/149‑1 (MQ=255)
           aCGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggtgg                                                                                                                                        <  3:264295/149‑1 (MQ=255)
              cgGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCa                                                                                                                                     <  1:57523/149‑1 (MQ=255)
                 ggATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCg                                                                                                                                  <  7:164233/149‑1 (MQ=255)
                            tgtTCCGCTACCCGAGTCACCCGAATGTCACCTACCTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCAACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTAcc                                                                                                                       <  8:7018/149‑1 (MQ=255)
                              ttgcGCTACCCGTGTGACCCGATTGTCTACTAGATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTAccc                                                                                                                      <  2:368937/145‑1 (MQ=255)
                              ttCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCt                                                                                                                     >  1:91208/1‑149 (MQ=255)
                                ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTgg                                                                                                                   >  5:369468/1‑149 (MQ=255)
                                ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                                    <  4:199143/148‑1 (MQ=255)
                                 cGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGCGGGCAGCGAGCCGGGTACCCTggg                                                                                                                  <  8:314430/149‑1 (MQ=255)
                                  gCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                                    >  2:339090/1‑146 (MQ=255)
                                  gCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                                    <  1:339090/146‑1 (MQ=255)
                                      cccGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGt                                                                                                              <  5:238718/148‑1 (MQ=255)
                                      cccGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTg                                                                                                             <  6:23031/149‑1 (MQ=255)
                                        cGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCt                                                                                                           <  7:149647/149‑1 (MQ=255)
                                         gAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTc                                                                                                          >  8:246734/1‑149 (MQ=255)
                                          aGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCa                                                                                                         <  5:201903/149‑1 (MQ=255)
                                              aCCCGAATGTCAACTACATCAAGCTTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAAGAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGcag                                                                                                     >  8:299308/1‑147 (MQ=255)
                                                ccGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGt                                                                                                    >  6:299438/1‑148 (MQ=255)
                                                      gTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGaa                                                                                                                                                  <  8:160917/96‑1 (MQ=255)
                                                      gTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGaa                                                                                                                                                  >  7:160917/1‑96 (MQ=255)
                                                       tCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGaa                                                                                            <  6:369468/149‑1 (MQ=255)
                                                          aCTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAg                                                                                         >  3:118422/1‑149 (MQ=255)
                                                               aTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCgg                                                                                    <  5:299438/149‑1 (MQ=255)
                                                                 cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggtg                                                                                                                                         <  4:24280/94‑1 (MQ=255)
                                                                 cAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggtg                                                                                                                                         >  3:24280/1‑94 (MQ=255)
                                                                      gtgtGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGcc                                                                             <  2:91208/149‑1 (MQ=255)
                                                                           gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACATCTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAaca                                                                        >  1:119237/1‑149 (MQ=255)
                                                                              ggCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACAcc                                                                      <  6:155330/148‑1 (MQ=255)
                                                                               gCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCt                                                                     <  4:118422/148‑1 (MQ=255)
                                                                                  tGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATc                                                                 >  4:88200/1‑149 (MQ=255)
                                                                                  tGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATc                                                                 >  2:357513/1‑149 (MQ=255)
                                                                                                 tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGAt                                                                  >  1:180034/1‑133 (MQ=255)
                                                                                                 tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGAt                                                                  <  2:180034/133‑1 (MQ=255)
                                                                                                  ccGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGcc                                                 >  5:162705/1‑149 (MQ=255)
                                                                                                                    gCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGccgccg                                >  7:7208/1‑148 (MQ=255)
                                                                                                                         tGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATTCCGCCGGACcag                          >  7:74115/1‑149 (MQ=255)
                                                                                                                          gTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcag                          >  2:65444/1‑148 (MQ=255)
                                                                                                                           ttCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcag                          <  1:357513/147‑1 (MQ=255)
                                                                                                                            tCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcagcag                       >  7:89359/1‑149 (MQ=255)
                                                                                                                              ggcAACGGGCTGGCCGTTGACGAACATCTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACAACTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACAAGCAGTg                     >  8:283711/3‑149 (MQ=255)
                                                                                                                              ggcAACGGGCTGGCCGTTGACGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg                     >  6:166194/3‑149 (MQ=255)
                                                                                                                              gTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTg                     <  8:7208/149‑1 (MQ=255)
                                                                                                                                   cGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAac                                                                         >  8:273411/1‑92 (MQ=255)
                                                                                                                                   cGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAac                                                                         <  7:273411/92‑1 (MQ=255)
                                                                                                                                    ggCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGAGCGGGGCGGCGGAACACGTGAGCCGCAGGGAAATGGGCCGTTTGCCCATGCCGCCGGACCAGCAGTGGACCGt               >  6:171682/1‑149 (MQ=255)
                                                                                                                                          cccGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGc         >  4:66943/1‑149 (MQ=255)
                                                                                                                                          cccGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGc         >  6:145819/1‑149 (MQ=255)
                                                                                                                                            cGTTGACGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCgg       <  5:166194/149‑1 (MQ=255)
                                                                                                                                                 ccGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCGGGCCAt  <  6:162705/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCGGGCCAT  >  NC_002947/1633418‑1633711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑