Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,655,522 A→T 7.8% L416Q (CTG→CAG) ‡ PP_1449 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,655,5220AT 7.8% 142.7 / 2.3 51L416Q (CTG→CAG) ‡PP_1449hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/19);  new base T (2/2);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

CTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTGCG  >  NC_002947/1655377‑1655670
                                                                                                                                                  |                                                                                                                                                     
cTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGc                                                                                                                                                    <  6:108855/149‑1 (MQ=255)
        aCATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCg                                                                                                                                            >  6:83061/1‑149 (MQ=255)
            gtgCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCACTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGt                                                                                                                                        >  7:302145/1‑149 (MQ=255)
             tgCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTc                                                                                                                                       >  3:353373/1‑149 (MQ=255)
             tgCCCGGCGCTCAGGGTACTGCCCTTTGAGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑AGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACAGCTGTc                                                                                                                                       >  3:347774/1‑149 (MQ=255)
               cccGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGCatc                                                                                                                                             >  1:17180/1‑139 (MQ=255)
               cccGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGCatc                                                                                                                                             <  2:17180/141‑3 (MQ=255)
                      cTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCACTCGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGt                                                                                                                                 >  1:279589/1‑147 (MQ=255)
                       tCAGGGTACTGAAATTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGAGCAATAAAACCATGCGATGACGCACCTGCTGTTCGCTGCGTTTGCTGTCGATGCCGGCGAGGATGTCAAGGCCGGCACCGCTGTCAACAGTCAg                                                                                                                              >  5:165310/1‑148 (MQ=255)
                        cAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCt                                                                                                                            >  7:318917/1‑149 (MQ=255)
                        cAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCt                                                                                                                            >  7:264670/1‑149 (MQ=255)
                         aGGGTAATGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTg                                                                                                                                         >  5:266950/1‑135 (MQ=255)
                          gggTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGCatc                                                                                                                                             >  2:288863/1‑128 (MQ=255)
                          gggTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGAACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCCTGGACATCCTCGCCGGCatc                                                                                                                                             <  1:288863/130‑3 (MQ=255)
                            gTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGtt                                                                                                                        <  8:47595/149‑1 (MQ=255)
                              aCTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTAc                                                                                                                      >  2:73518/1‑149 (MQ=255)
                                  cccTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCAAGGCAGGCACCGCTGTCGACCg                                                                                                                                  >  7:196097/1‑133 (MQ=255)
                                       tGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTcc                                                                                                              <  1:953/148‑1 (MQ=255)
                                         cGGTTTCCTTGAGCTGGTCGCTATCGCTGTCCC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCAcc                                                                                                           >  5:11462/1‑149 (MQ=255)
                                         cGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCAcc                                                                                                           <  2:82353/149‑1 (MQ=255)
                                          ggTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCt                                                                                                          >  6:287839/1‑149 (MQ=255)
                                              tCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTcg                                                                                                       <  1:73518/148‑1 (MQ=255)
                                                   gAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCgcggc                                                                                                   <  2:279589/147‑1 (MQ=255)
                                                   gAGCTGGTCACTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCgcggc                                                                                                   <  8:302145/147‑1 (MQ=255)
                                                         gTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATgc                                                                                           <  8:264670/149‑1 (MQ=255)
                                                         gTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATgc                                                                                           <  5:287839/149‑1 (MQ=255)
                                                          tCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGGCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATgcg                                                                                          >  5:283489/1‑149 (MQ=255)
                                                                  gCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAg                                                                                                                              <  1:119862/105‑1 (MQ=255)
                                                                  gCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAg                                                                                                                              >  2:119862/1‑105 (MQ=255)
                                                                      tCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTcc                                                                                                              <  8:298016/117‑1 (MQ=255)
                                                                      tCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTcc                                                                                                              >  7:298016/1‑117 (MQ=255)
                                                                        aC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCg                                                                             >  5:167168/1‑148 (MQ=255)
                                                                                 aTAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCaa                                                                     >  5:346651/1‑148 (MQ=255)
                                                                                             gATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGCCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCtctct                                                       <  6:283489/149‑2 (MQ=255)
                                                                                                   gCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGAGGTCAAGCGCATGTCGTCGCCGGACTGCTACCG‑CACGCTCTCGGTGcc                                                  >  8:312111/1‑148 (MQ=255)
                                                                                                          ctgTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCGACAG‑CACGCTCTCAGTGCCCAGCAAtt                                          <  8:318917/149‑1 (MQ=255)
                                                                                                           tgTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAAtt                                          >  1:202054/1‑148 (MQ=255)
                                                                                                             ttCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑c                                                                >  4:170607/1‑124 (MQ=255)
                                                                                                             ttCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑c                                                                <  3:170607/124‑1 (MQ=255)
                                                                                                                           tGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGc                         >  8:175170/1‑149 (MQ=255)
                                                                                                                            gTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCCTTACCGGCGTCCACCTTCGCGCCGGTCATGCGCATGTCGTCGCCGGACAGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCt                        >  3:118096/1‑149 (MQ=255)
                                                                                                                             tCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGc                         <  2:202054/147‑1 (MQ=255)
                                                                                                                                 tGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGGCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTgtgg                   <  6:11462/149‑1 (MQ=255)
                                                                                                                                   ccGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAGCCACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTgtgg                   <  1:81626/148‑1 (MQ=255)
                                                                                                                                      gCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGggtggt              >  8:214108/1‑149 (MQ=255)
                                                                                                                                      gCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGggtggt              <  6:167168/149‑1 (MQ=255)
                                                                                                                                         aGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGggtggtggt           >  3:151347/1‑149 (MQ=255)
                                                                                                                                           gATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGggtggtggtgg         >  8:55758/1‑149 (MQ=255)
                                                                                                                                              gTCCATGCCGGCACCGCTGTCGCCCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGGGGTGGTGGTcg      >  4:241739/1‑148 (MQ=255)
                                                                                                                                                ccAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGTCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTg    >  5:336316/1‑149 (MQ=255)
                                                                                                                                                 cAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTg    <  5:83061/148‑1 (MQ=255)
                                                                                                                                                  aGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCGCAGTGCCCAGCAATTGACTGCCCGGTGGTTGCTTGTGGGTGGTGGTGGTCTTGCg  >  5:314844/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CTTTATCGACATGTGCCCGGCGCTCAGGGTACTGCCCTTTGCGGTTTCCTTGAGCTGGTCGCTATCGCTGTCAC‑CGCGCCATAAATCCTTGCGATGACGCACCTGCTGTTCGGTGCGTTTGCTGTCGATGCCGGCGAGGATGTCCAGGCCGGCACCGCTGTCGACCGTCAGCTCGTTACCGGCGTCCACCTTCGCGGCGGTCATGCGCATGTCGTCGCCGGACTGCAACAG‑CACGCTCTCAGTGCCCAGCAATTGACTGCCCTGTTGTTGCTTGTGGGTGGTGGTGGTCTTGCG  >  NC_002947/1655377‑1655670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑