Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,688,583 G→T 7.8% A318A (GCC→GCA)  ydcT ← polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,688,5830GT 7.8% 159.5 / 4.0 51A318A (GCC→GCA) ydcTpolyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base T (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

AACAGCAGTGCCAGGTACAGGTTGGGGCGCCGGTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACATCGTGCAGCACGCCGCTGACCTGTACCTCATGGCTGGTCA  >  NC_002947/1688436‑1688728
                                                                                                                                                   |                                                                                                                                                 
aaCAGCAGTGCCAGGTACAGGTTGGGGCGCCGGTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAgg                                                                                                                                                  >  3:102918/1‑149 (MQ=255)
       gTGCCAGGTACAGGTTGGGGCGCCGGTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCAt                                                                                                                                           <  4:102918/149‑1 (MQ=255)
           cAGGTACAGGTTGGGGCGCCGGTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGc                                                                                                                                       <  4:239936/149‑1 (MQ=255)
                    gTTGGGGCGCCGGTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAg                                                                                                                              >  3:34069/1‑149 (MQ=255)
                              cGGTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTgg                                                                                                                    >  5:143417/1‑149 (MQ=255)
                                gTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGc                                                                                                                   >  4:142506/1‑148 (MQ=255)
                                gTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGc                                                                                                                   <  3:142506/148‑1 (MQ=255)
                                    agcagGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGc                                                                                                              >  3:128420/1‑149 (MQ=255)
                                    agcagGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGc                                                                                                              >  8:217561/1‑149 (MQ=255)
                                    agcagGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGc                                                                                                              <  5:178859/149‑1 (MQ=255)
                                        ggTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCt                                                                                                                 <  4:78634/142‑1 (MQ=255)
                                        ggTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCt                                                                                                                 >  3:78634/1‑142 (MQ=255)
                                         gTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCATGCCTGCATCTGCTTCTGCCAGCGGTCAtt                                                                                                                      <  6:295762/136‑1 (MQ=255)
                                         gTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCATGCCTGCATCTGCTTCTGCCAGCGGTCAtt                                                                                                                      >  5:290810/1‑136 (MQ=255)
                                         gTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCATGCCTGCATCTGCTTCTGCCAGCGGTCAtt                                                                                                                      >  5:295762/1‑136 (MQ=255)
                                         gTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCATGCCTGCATCTGCTTCTGCCAGCGGTCAtt                                                                                                                      <  6:290810/136‑1 (MQ=255)
                                             gACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGc                                                                                                       >  7:232332/1‑147 (MQ=255)
                                                aGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCcag                                                                                                    >  4:59909/1‑147 (MQ=255)
                                                 gggTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGc                                                                                                       >  5:228242/1‑143 (MQ=255)
                                                 gggTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGc                                                                                                       <  6:228242/143‑1 (MQ=255)
                                                 gggTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCcagcag                                                                                                 <  7:217561/149‑1 (MQ=255)
                                                  ggTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGt                                                                                                <  1:96642/149‑1 (MQ=255)
                                                      cgcAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGa                                                                                             <  2:59783/148‑1 (MQ=255)
                                                        cAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCg                                                                                          >  4:321593/1‑149 (MQ=255)
                                                           tgtgCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTg                                                                                       <  6:143417/149‑1 (MQ=255)
                                                             tgCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTc                                                                                     <  4:34069/149‑1 (MQ=255)
                                                               cTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTg                                                                                                                     >  1:246444/1‑115 (MQ=255)
                                                               cTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTg                                                                                                                     <  2:246444/115‑1 (MQ=255)
                                                               cTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCa                                                                                   <  2:200685/149‑1 (MQ=255)
                                                                tCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAg                                                                                  >  8:207405/1‑149 (MQ=255)
                                                                tCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAg                                                                                  >  5:344308/1‑149 (MQ=255)
                                                                 cATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGc                                                                                 >  4:203762/1‑149 (MQ=255)
                                                                  aTGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGc                                                                                 <  3:59909/148‑1 (MQ=255)
                                                                   tGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGGCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTg                                                                               >  5:48892/1‑149 (MQ=255)
                                                                           gCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCt                                                                                                                                      >  3:353026/1‑86 (MQ=255)
                                                                           gCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCt                                                                                                                                      <  4:353026/86‑1 (MQ=255)
                                                                                 ggTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCgg                                                                  <  4:128420/148‑1 (MQ=255)
                                                                                             caCCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCtt                                                     >  1:251309/1‑149 (MQ=255)
                                                                                              aCCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTg                                                    <  7:207405/149‑1 (MQ=255)
                                                                                                 gTCATGGCCTCGCGGGGCCAGGGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTa                                                 <  4:79301/149‑1 (MQ=255)
                                                                                                    aTGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTg                                              <  6:131373/149‑1 (MQ=255)
                                                                                                      ggCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACt                                               >  7:269898/1‑146 (MQ=255)
                                                                                                      ggCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACt                                               <  8:269898/146‑1 (MQ=255)
                                                                                                        ccTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACa                                          >  8:103246/1‑149 (MQ=255)
                                                                                                        ccTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACa                                          <  3:203762/149‑1 (MQ=255)
                                                                                                         cTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGtt                                                                                        >  4:51211/1‑102 (MQ=255)
                                                                                                                       gcgcCTGCAGGCGTTGCCCGAGCTGCCAGGACTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACATCGTGCAGCAcgccg                           >  1:24523/1‑149 (MQ=255)
                                                                                                                             gCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCgg                                                                  <  6:127119/104‑1 (MQ=255)
                                                                                                                             gCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCgg                                                                  >  5:127119/1‑104 (MQ=255)
                                                                                                                                         cGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACATCGTGCAGCACGCCGCTGACCTGTACCTCATgg         >  3:63729/1‑149 (MQ=255)
                                                                                                                                               gCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACATCGTGCAGCACGCCGCTGACCTGTACCTCATGGCTGGt    >  4:65396/1‑148 (MQ=255)
                                                                                                                                                ccAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACATCGTGCAGCACGCCGCTGACCTGTACCTCATGGCTGGTCa  >  7:200462/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACAGCAGTGCCAGGTACAGGTTGGGGCGCCGGTACAGCAGGTTGGACAGGGTGCGCAGTGTGCTCATGTCAGCGGCCCTCGGTTTCCTGCAGCACCGTCATGGCCTCGCGGGGCCAGTGCGCCTGCAGGCGTTGCCCGAGCTGCCAGGCCTGCATCTGCTTCTGCCAGCGGTCATTGGCCTGGCTTACTGCCAGCAGTTGACCGTTGTCCAGCTGCAGTTCGTAGCGGGTGGCGCTGCCTTGGTACTGGACATCGTGCAGCACGCCGCTGACCTGTACCTCATGGCTGGTCA  >  NC_002947/1688436‑1688728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑