Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,786,961	C→T	8.7%	G264G (GGC→GGT)	tsf →	elongation factor Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,786,961	0	C	T	8.7%	192.9 / 5.6	68	G264G (GGC→GGT)	tsf	elongation factor Ts
Reads supporting (aligned to +/- strand):  ref base C (28/34);  new base T (3/3);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAG  >  NC_002947/1786814‑1787102                                                                                                                                                    |                                                                                                                                               gaACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGc                                                                                                                                                <  3:237928/148‑1 (MQ=255)   aCATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGaa                                                                                                                                             <  4:211883/149‑1 (MQ=255)    cATGATCATCGGTCGTATCACCAAGTTCAAGGCCGTATCCTCGCTGAAAGAGCAAGCCTTCGTCATGAAGCCGGAAATCTAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGATATCGTTTCCTTCACCTACTTCAAAGTAGCCGAAg                                                                                                                                            <  7:341000/149‑1 (MQ=255)    cATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCGCCTACTTTGAAGTAGGTGAAg                                                                                                                                            <  3:267759/149‑1 (MQ=255)           aaCGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                                       >  1:70518/1‑147 (MQ=255)            aCGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCgag                                                                                                                                    <  3:116757/149‑1 (MQ=255)                     tCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTc                                                                                                                           <  2:70518/149‑1 (MQ=255)                       aCCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcga                                                                                                                         >  2:46673/1‑149 (MQ=255)                        ccAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcgac                                                                                                                        <  8:329949/149‑1 (MQ=255)                        ccAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcgac                                                                                                                        >  6:131526/1‑149 (MQ=255)                        ccAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGATGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcgac                                                                                                                        >  2:334149/1‑149 (MQ=255)                          aaGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcgacga                                                                                                                      <  5:79714/149‑1 (MQ=255)                             ttCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACtt                                                                                                                   >  6:253181/1‑149 (MQ=255)                                aaGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCgc                                                                                                                <  4:203321/149‑1 (MQ=255)                                 aGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCgct                                                                                                               <  1:139329/149‑1 (MQ=255)                                          ccTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGaa                                                                                                         >  8:106950/1‑146 (MQ=255)                                          ccTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGaa                                                                                                         <  7:106950/146‑1 (MQ=255)                                          ccTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGACATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGtt                                                                                                      <  3:245065/149‑1 (MQ=255)                                            tCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGc                                                                                                    >  5:383525/1‑149 (MQ=255)                                              gCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGCCGACTTCGCTGCTGACGTTGCCg                                                                                                  >  8:291136/1‑149 (MQ=255)                                              gCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGCTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGcc                                                                                                   <  1:334149/148‑1 (MQ=255)                                                  aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCa                                                                                              <  7:87542/149‑1 (MQ=255)                                                     gagCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGt                                                                                           >  8:45733/1‑149 (MQ=255)                                                       gCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                                       <  6:131692/103‑1 (MQ=255)                                                       gCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                                       >  5:131692/1‑103 (MQ=255)                                                           gCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCgctgc                                                                                     <  1:46673/149‑1 (MQ=255)                                                              ttCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCtgctgc                                                                                  >  7:305619/1‑149 (MQ=255)                                                                cGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCtgctgc                                                                                  <  6:383525/147‑1 (MQ=255)                                                                cGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCtgctgc                                                                                  <  6:207092/147‑1 (MQ=255)                                                                 gTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCaa                                                                               <  3:302067/149‑1 (MQ=255)                                                                  tCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAg                                                                              >  2:254146/1‑149 (MQ=255)                                                                     tGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAg                                                                           >  6:169995/1‑149 (MQ=255)                                                                      gAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAg                                                                           >  5:268964/1‑148 (MQ=255)                                                                            ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAg                                                                           <  8:285116/142‑1 (MQ=255)                                                                            ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTagatcg                                                                    >  7:285116/1‑144 (MQ=255)                                                                            ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACa                                                                    >  8:294552/1‑149 (MQ=255)                                                                              aaaTCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGATGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGc                                                                  <  1:254146/149‑1 (MQ=255)                                                                                   aaGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCg                                                              >  8:154841/1‑148 (MQ=255)                                                                                     ggTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCATGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑Ct                                                          >  2:19234/1‑149 (MQ=255)                                                                                     ggTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑Ct                                                          <  8:102077/149‑1 (MQ=255)                                                                                       ttGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGt                                                        >  6:186874/1‑149 (MQ=255)                                                                                          gCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGc                                                     <  5:186874/149‑1 (MQ=255)                                                                                                  gCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCaaa                                               >  2:165774/1‑147 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCaaa                                               <  1:3426/145‑1 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCaaa                                               >  2:3426/1‑145 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAgg                                           <  6:23768/149‑1 (MQ=255)                                                                                                           gCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGGCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCg                                    <  7:291136/149‑1 (MQ=255)                                                                                                              ggTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTc                                 <  1:165774/149‑1 (MQ=255)                                                                                                                  cAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCAcgc                             >  8:62461/1‑149 (MQ=255)                                                                                                                   aGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGt                                                        >  3:299566/1‑121 (MQ=255)                                                                                                                   aGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGt                                                        <  4:299566/121‑1 (MQ=255)                                                                                                                      aaTCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCAcgcgcg                          >  5:221415/1‑148 (MQ=255)                                                                                                                       aTCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCa                        <  5:169995/149‑1 (MQ=255)                                                                                                                        tCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAg                       >  2:221385/1‑149 (MQ=255)                                                                                                                           atgccTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCct                    >  2:76384/4‑149 (MQ=255)                                                                                                                             tCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCctc                   <  7:45733/148‑1 (MQ=255)                                                                                                                               cTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAg                       >  5:49426/1‑142 (MQ=255)                                                                                                                               cTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAg                       <  6:49426/142‑1 (MQ=255)                                                                                                                               cTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                >  7:261851/1‑149 (MQ=255)                                                                                                                                  cgccTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTACTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                <  1:76384/145‑1 (MQ=255)                                                                                                                                   aCCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGT‑AGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCa            >  2:159636/1‑148 (MQ=255)                                                                                                                                       aCTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAAc        >  8:61746/1‑149 (MQ=255)                                                                                                                                        cTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCaaaa         <  5:253181/147‑1 (MQ=255)                                                                                                                                           cAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACggg     <  2:105787/148‑1 (MQ=255)                                                                                                                                           cAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgg      <  6:268964/147‑1 (MQ=255)                                                                                                                                            aaaGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgggg    <  7:62461/148‑1 (MQ=255)                                                                                                                                            aaaGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGgga   <  7:294552/149‑1 (MQ=255)                                                                                                                                            aaaGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGgga   <  6:74038/149‑1 (MQ=255)                                                                                                                                              aGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCACGCTCACGCGCGCAGCCTCTTTGTCAAAACGGggag  >  1:828/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                               GAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGT‑CTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAG  >  NC_002947/1786814‑1787102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑