Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,831,780	T→C	9.4%	L220P (CTG→CCG)	yfiP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,831,780	0	T	C	9.4%	177.6 / 7.7	64	L220P (CTG→CCG)	yfiP	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/31);  new base C (3/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

CGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGA  >  NC_002947/1831635‑1831925                                                                                                                                                  |                                                                                                                                                  cggcgCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGcc                                                                                                                                                <  4:299947/149‑1 (MQ=255) cggcgCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGc                                                                                                                                                 <  5:152358/148‑1 (MQ=255)     gcTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCtt                                                                                                                                            <  6:177746/149‑1 (MQ=255)       tCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTgc                                                                                                                                          >  4:124240/1‑148 (MQ=255)         cAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGa                                                                                                                                          <  3:251667/147‑1 (MQ=255)         cAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTGCCg                                                                                                                                        <  3:124240/149‑1 (MQ=255)         cAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGAGGTGACTGGACCTTGCTGGAGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTAACTGAGCGGCAAGCGGCGGCTGCCTCTTgcc                                                                                                                                         >  2:276549/1‑146 (MQ=255)            gcgcGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGc                                                                                                                                     <  1:45733/149‑1 (MQ=255)             cgcgATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCa                                                                                                                                    <  7:247375/149‑1 (MQ=255)              gcgATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCTATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTTGATGTGTTCAGCCTGAATTACCTGAGCGGCAAGAGGCGGCTGCCGCTTGACGAGCAg                                                                                                                                   >  7:235843/1‑149 (MQ=255)              gcgATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGCTCGTCAAGCGGCAGCCGCCGCTTGCCgctcag                                                                                                                                   <  7:265631/149‑6 (MQ=38)                   gatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgacga                                                                                                                              >  7:327662/1‑149 (MQ=255)                     tCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGaca                                                                                                                            >  8:256600/1‑149 (MQ=255)                       aTTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCAGCTTGACGAGCAGGACGAcacc                                                                                                                          >  7:327114/1‑149 (MQ=255)                              tACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACccacca                                                                                                                     <  8:29972/147‑1 (MQ=255)                              tACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGc                                                                                                                   >  6:349011/1‑149 (MQ=255)                                 ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACccacca                                                                                                                     >  7:321908/1‑144 (MQ=255)                                 ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACccacca                                                                                                                     <  8:321908/144‑1 (MQ=255)                                 ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGtt                                                                                                                <  2:165651/149‑1 (MQ=255)                                  gCAGAAGTTGCGGCGATGTGCCGGGACCTGGCGGGCGATGGCCAGGCGTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGttt                                                                                                               <  6:272864/149‑1 (MQ=255)                                    aGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGc                                                                                                             <  7:256600/149‑1 (MQ=255)                                    aGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGc                                                                                                             <  5:203819/149‑1 (MQ=255)                                      aaGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCAt                                                                                                           <  4:12936/149‑1 (MQ=255)                                       aGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATa                                                                                                          <  5:284001/149‑1 (MQ=255)                                           gcggcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCAt                                                                                                           <  8:73801/144‑1 (MQ=255)                                           gcggcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCAt                                                                                                           >  7:73801/1‑144 (MQ=255)                                            cggcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTc                                                                                                     <  8:11387/149‑1 (MQ=255)                                            cggccATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGCGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTc                                                                                                     <  4:8470/149‑1 (MQ=255)                                                          gACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGACGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCtata                                                                                                <  4:350157/140‑1 (MQ=255)                                                          gACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGACGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCtata                                                                                                >  3:350157/1‑140 (MQ=255)                                                                gCTGTCGATTGCCAGGCTTCGCAGGCGCTTGATGACTATCTTGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGcac                                                                                   <  8:327662/147‑1 (MQ=255)                                                                  tGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCCGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAAcc                                                                               >  2:8185/1‑149 (MQ=255)                                                                     cGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCt                                                                            >  4:358733/1‑149 (MQ=255)                                                                           ccAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTg                                                                      >  4:79075/1‑149 (MQ=255)                                                                            cAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTgg                                                                     >  7:70458/1‑149 (MQ=255)                                                                             aGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGt                                                                    >  3:182485/1‑149 (MQ=255)                                                                                  tCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCAt                                                                >  4:305773/1‑148 (MQ=255)                                                                                        gcgcTGGTTGCCTATATGTATGTGTTCTGCCTTCATTACATGCGCGTCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCATAACCCCTGCTTTGGTTCATAATGACg                                                         <  8:235843/149‑1 (MQ=255)                                                                                              gATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACAGAGCTg                                                   >  3:12295/1‑149 (MQ=255)                                                                                                tGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCCGCAAGCTGCGGCTCCCTCTTGACGAGCAGGACGACACCCACCAGCGTATGCATACATTCCAATAGTCAAAGGAGCACAACCCCTGCTTTGGTTCATAATGACCGAGCTgg                                                  >  4:214361/1‑148 (MQ=255)                                                                                                 gCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTgg                                                  >  4:182134/1‑147 (MQ=255)                                                                                                  ccTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACAGAGCTGGCa                                                <  4:12295/148‑1 (MQ=255)                                                                                                         ggATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGt                                        >  7:459/1‑149 (MQ=255)                                                                                                          gATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAggg                                         <  5:349011/147‑1 (MQ=255)                                                                                                          gATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAg                                           <  8:70458/145‑1 (MQ=255)                                                                                                               gtTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGc                                    >  7:275334/1‑147 (MQ=255)                                                                                                               gtTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCga                                  <  7:138195/149‑1 (MQ=255)                                                                                                                ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCa                                                >  4:29903/1‑134 (MQ=255)                                                                                                                ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCa                                                <  3:29903/134‑1 (MQ=255)                                                                                                                  cAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGAc                               >  1:201647/1‑149 (MQ=255)                                                                                                                       tGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAg                          >  4:169580/1‑149 (MQ=255)                                                                                                                             aCCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTg                                                                      >  7:61236/1‑99 (MQ=255)                                                                                                                             aCCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTg                                                                      <  8:61236/99‑1 (MQ=255)                                                                                                                               cTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTc                                                                                                     <  6:18248/66‑1 (MQ=255)                                                                                                                               cTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTc                                                                                                     >  5:18248/1‑66 (MQ=255)                                                                                                                               cTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCAt                                                                >  4:272797/1‑103 (MQ=255)                                                                                                                               cTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCAt                                                                <  3:272797/103‑1 (MQ=255)                                                                                                                               cTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTg                  >  5:153208/1‑149 (MQ=255)                                                                                                                                      gCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTATGCATACCTTCCTATAGTCAAAGGGCCACAACCCCTGCTTTGGTTCATAAAGACGGAGCTGGCAGCAAGGGTGGGCGACACGGCAGGGCGATTGGCTTGa            >  3:133532/1‑148 (MQ=255)                                                                                                                                          gcggcaGCCGCCGCTTGCCGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAgg                                          <  2:167612/114‑1 (MQ=255)                                                                                                                                          gcggcaGCCGCCGCTTGCCGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAgg                                          >  1:167612/1‑114 (MQ=255)                                                                                                                                           cggcggCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTccc      <  3:182134/149‑1 (MQ=255)                                                                                                                                             gcgtcTGCCGCTTGACTAGTATGACGACACCAACCAGGGTTTGCTTACCTTCCTAAAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCtt    <  8:327114/149‑1 (MQ=255)                                                                                                                                               ggcTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTTCATACCTTCCTATAGTCCAATGGGCACAACCCCTGCTTTGGTTGATAATGACGGAGCTGGCAGCCAGGGTGTGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGa  <  3:214361/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGA  >  NC_002947/1831635‑1831925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑