Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,844,700	A→T	5.5%	W32R (TGG→AGG)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,844,700	0	A	T	5.5%	220.5 / 2.1	72	W32R (TGG→AGG)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base A (31/37);  new base T (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGTCAAGTGGTAACGGCCAAGCCGCGA  >  NC_002947/1844555‑1844848                                                                                                                                                  |                                                                                                                                                       gCCTGCTGCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGt                                                                                                                                                     <  6:126797/149‑1 (MQ=255)  ccTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCTGGt                                                                                                                                                     >  5:226599/1‑148 (MQ=255)  ccTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCTGGt                                                                                                                                                     >  5:221365/1‑148 (MQ=255)  ccTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGt                                                                                                                                                     >  8:66161/1‑148 (MQ=255)    tGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGa                                                                                                                                                   >  4:13619/1‑148 (MQ=255)     gCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGaaa                                                                                                                                                 >  5:143624/1‑149 (MQ=255)         cAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAg                                                                                                                                                       >  1:266355/1‑139 (MQ=255)         cAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAg                                                                                                                                                       <  2:266355/139‑1 (MQ=255)         cAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑c                                                                                                                                           >  7:271399/1‑149 (MQ=255)         cAGGGTTTGTGCGGCAATTCTCTACAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGAGGCGAGGTGAAATAG‑‑c                                                                                                                                           >  4:218599/1‑149 (MQ=255)            ggTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTAc                                                                                                                                        <  1:324922/149‑1 (MQ=255)                tgtgCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCa                                                                                                                                     <  8:238485/148‑1 (MQ=255)                       cAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAgg                                                                                                                             <  6:143624/149‑1 (MQ=255)                         aTTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGc                                                                                                                           >  1:134478/1‑149 (MQ=255)                                 cAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAgc                                                                                                                   <  3:218599/149‑1 (MQ=255)                                      gcgcAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAgccggcc                                                                                                              >  7:250062/1‑149 (MQ=255)                                        gcAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGccggccgg                                                                                                            >  5:139293/1‑149 (MQ=255)                                        gcAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGccggccgg                                                                                                            >  1:9325/1‑149 (MQ=255)                                        gcAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGccggccgg                                                                                                            <  8:271399/149‑1 (MQ=255)                                         cAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGt                                                                                                           <  3:50987/149‑1 (MQ=255)                                            gcgcGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAg                                                                                                         >  6:359780/1‑148 (MQ=255)                                                 aGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGc                                                                                                   <  4:104651/149‑1 (MQ=255)                                                   ccGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGcc                                                                                                  <  8:250062/148‑1 (MQ=255)                                                    cGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCa                                                                                                                                     <  7:328203/112‑1 (MQ=255)                                                    cGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCa                                                                                                                                     >  8:328203/1‑112 (MQ=255)                                                    cGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcag                                                                                                <  2:134478/149‑1 (MQ=255)                                                       gCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcag                                                                                             <  3:221575/149‑1 (MQ=255)                                                       gCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcag                                                                                             <  6:380084/149‑1 (MQ=255)                                                        ccATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcag                                                                                             >  8:316543/1‑148 (MQ=255)                                                         cATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcagca                                                                                           <  3:13619/149‑1 (MQ=255)                                                         cATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcagca                                                                                           <  2:97736/149‑1 (MQ=255)                                                         cATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcag                                                                                             >  3:137107/1‑147 (MQ=255)                                                             gCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTAc                                                                                       >  1:36320/1‑149 (MQ=255)                                                              cGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAAt                                                                                                                                                >  8:158668/1‑93 (MQ=255)                                                              cGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAAt                                                                                                                                                <  7:158668/93‑1 (MQ=255)                                                               gggTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTAccc                                                                                     <  4:181943/149‑1 (MQ=255)                                                                 gTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCgg                                                                                   >  7:250714/1‑149 (MQ=255)                                                                   gCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCt                                                                                 >  4:46941/1‑149 (MQ=255)                                                                      gCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCg                                                                              <  4:110013/149‑1 (MQ=255)                                                                        cAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGa                                                                            <  7:4163/149‑1 (MQ=255)                                                                           gggCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTc                                                                         <  3:102623/149‑1 (MQ=255)                                                                               gcCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCTGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCaa                                                                     <  6:221365/149‑1 (MQ=255)                                                                               gcCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCTGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCaa                                                                     <  6:226599/149‑1 (MQ=255)                                                                               gcCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGc                                                                               >  8:335867/1‑139 (MQ=255)                                                                               gcCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGc                                                                               <  7:335867/139‑1 (MQ=255)                                                                                 cAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGc                                                                   <  7:66161/149‑1 (MQ=255)                                                                                          ggCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGc                                                           <  3:46941/148‑1 (MQ=255)                                                                                           gCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcag                                                                                             >  1:299803/1‑113 (MQ=255)                                                                                           gCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcag                                                                                             <  2:299803/113‑1 (MQ=255)                                                                                           gCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGca                                                          >  2:107337/1‑148 (MQ=255)                                                                                                 atcTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGc                                                   >  1:322202/1‑149 (MQ=255)                                                                                                       ccGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAg                                                                                                      >  2:183633/1‑92 (MQ=255)                                                                                                       ccGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAg                                                                                                      <  1:183633/92‑1 (MQ=255)                                                                                                       ccGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCctcctc                                             <  4:353267/149‑1 (MQ=255)                                                                                                         gcgcTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTg                                           <  8:34134/149‑1 (MQ=255)                                                                                                           gcTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcagca                                                                                           <  7:136164/99‑1 (MQ=255)                                                                                                           gcTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCcagcagca                                                                                           >  8:136164/1‑99 (MQ=255)                                                                                                           gcTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAAAAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCGGAt                                         >  1:270111/1‑149 (MQ=255)                                                                                                             ggaagcagcGGGGGGGCCAGGTCGTTTGGTTGCAGCCAGGGTAAAAAGTTCTCACTAGCACCAGTGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGAt                                         <  1:120771/146‑1 (MQ=255)                                                                                                                agcagcTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGAGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAACCGATCGAGCACACTCGCCTCCTCTGATGTGcc                                    >  3:27123/1‑149 (MQ=255)                                                                                                                 gcagcTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGAtgtg                                      >  1:354377/1‑146 (MQ=255)                                                                                                                 gcagcTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGAtgtg                                      <  2:354377/146‑1 (MQ=255)                                                                                                                 gcagcTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCt                                   >  7:29490/1‑149 (MQ=255)                                                                                                                     cTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGAt                               <  7:316543/149‑1 (MQ=255)                                                                                                                      tGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATg                              >  2:154277/1‑149 (MQ=255)                                                                                                                       gggTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGTGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGc                             >  2:69704/1‑149 (MQ=255)                                                                                                                       gggTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGc                             >  7:10519/1‑149 (MQ=255)                                                                                                                          tGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGt                          <  2:322202/149‑1 (MQ=255)                                                                                                                                ggTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGTCAAGTg                    <  4:278957/149‑1 (MQ=255)                                                                                                                                ggTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGTCAAGTg                    <  8:29490/149‑1 (MQ=255)                                                                                                                                    gTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGTCAAGTGGTaa                <  7:259395/149‑1 (MQ=255)                                                                                                                                          ttGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGTCAAGTGGTAACgg             <  2:9325/146‑1 (MQ=255)                                                                                                                                                  aGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGTCAAGTGGTAACGGCCAAGCCGCGa  <  5:359780/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                       GCCTGCTCCAGGGTTTGTGCGGCAATTCTCTCCAGTTGCGCAGGCGCGAGCCGGGCCATGGCGGGTGCGGCCAGGGGCGCCAGTTGTTCGGCGATCATCTTGCCGCGCTGCAGCAGCTGGGTGCGCAGGTCGTTCTGTTGCAGCCAGGTGAAATAG‑‑CTACCCAGCACCAGGGCCATCAAGCCGGCCGGTAGCAGTGCCAGCAGCAGTACCCGGCTGCGGATTCCCAAGCGATCGAGCACACTCGCCTCCTCTGATGTGCCTGGATGCCGTCAAGTGGTAACGGCCAAGCCGCGA  >  NC_002947/1844555‑1844848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑