Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,873,895 G→C 6.7% G69A (GGC→GCC)  cobU → nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,873,8950GC 6.7% 192.5 / 3.9 60G69A (GGC→GCC) cobUnicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (27/29);  new base C (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CAGGCCCGCGCCCGTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGAC  >  NC_002947/1873747‑1874040
                                                                                                                                                    |                                                                                                                                                 
cAGGCCCGCGCCCGTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACgg                                                                                                                                                   >  8:190729/1‑149 (MQ=255)
      cgcgCCCGTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGt                                                                                                                                             <  4:35175/149‑1 (MQ=255)
        cgcCCGTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTc                                                                                                                                            >  5:364446/1‑148 (MQ=255)
          cccGTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGaa                                                                                                                                         <  5:208104/149‑1 (MQ=255)
          cccGTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGaa                                                                                                                                         <  5:291328/149‑1 (MQ=255)
             gTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGTTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAagag                                                                                                                                      >  3:217958/1‑149 (MQ=255)
              tCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGt                                                                                                                                             <  6:44392/141‑1 (MQ=255)
              tCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGt                                                                                                                                             >  5:44392/1‑141 (MQ=255)
              tCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAgg                                                                                                                                     <  5:304585/149‑1 (MQ=255)
                 gcagcaACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGTCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCa                                                                                                                                  >  8:52656/1‑149 (MQ=255)
                   agcaACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGGTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCAtc                                                                                                                                <  7:173506/149‑1 (MQ=255)
                    gcaACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCAtct                                                                                                                               <  2:249813/149‑1 (MQ=255)
                      aaCTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCg                                                                                                                             <  5:283644/149‑1 (MQ=255)
                      aaCTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCg                                                                                                                             <  2:373912/149‑1 (MQ=255)
                       aCTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCgg                                                                                                                            <  2:254518/149‑1 (MQ=255)
                        cTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGTTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGc                                                                                                                           <  4:217958/149‑1 (MQ=255)
                        cTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGc                                                                                                                           >  7:243074/1‑149 (MQ=255)
                        cTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGc                                                                                                                           <  7:82762/149‑1 (MQ=255)
                        cTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGc                                                                                                                           <  2:20282/149‑1 (MQ=255)
                           aCCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCt                                                                                                                         <  5:172352/148‑1 (MQ=255)
                             cAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATc                                                                                                                      >  1:1332/1‑149 (MQ=255)
                               aaCCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCg                                                                                                                    >  8:65189/1‑149 (MQ=255)
                                        gctcgcgcGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCACGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAATAGGGCCTCTCGGCCTATCCGCAGGCGGt                                                                                                            <  7:236274/148‑1 (MQ=255)
                                              tcgGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGc                                                                                                                           >  1:277556/1‑127 (MQ=255)
                                              tcgGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGc                                                                                                                           <  2:277556/127‑1 (MQ=255)
                                                 gCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCggg                                                                                                     >  1:240651/1‑146 (MQ=255)
                                                  ccAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCggg                                                                                                     >  3:54317/1‑145 (MQ=255)
                                                   cAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGTGACCACGGCGTGGTCGAAGAGGTCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGAt                                                                                                <  7:235616/149‑1 (MQ=255)
                                                    aaCTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATg                                                                                               <  2:237161/149‑1 (MQ=255)
                                                         gAAGCGCGGGCGATTCAGCTGGCAGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTa                                                                                            <  8:315062/147‑1 (MQ=255)
                                                                tGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTtc                                                                                   >  2:128187/1‑149 (MQ=255)
                                                                  gCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTtcg                                                                                  <  7:190729/148‑1 (MQ=255)
                                                                     aTTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGAAACTTCGTcgg                                                                              >  8:197921/1‑149 (MQ=255)
                                                                         aGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCa                                                                                                   >  4:181790/1‑124 (MQ=255)
                                                                         aGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCa                                                                                                   <  3:181790/124‑1 (MQ=255)
                                                                         aGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTcggcgg                                                                           <  6:364446/148‑1 (MQ=255)
                                                                         aGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCggg                                                                          <  8:243074/149‑1 (MQ=255)
                                                                          gctggctgGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCgggg                                                                         >  4:329559/1‑149 (MQ=255)
                                                                            tggctggcCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGgc                                                                       >  3:284242/1‑149 (MQ=255)
                                                                                tggcCTTCAGGGGGTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCgcgg                                                                   >  8:50287/1‑149 (MQ=255)
                                                                                tggcCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCgcgg                                                                   >  5:343083/1‑149 (MQ=255)
                                                                                          gggCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCg                                                                                                       <  8:12131/103‑1 (MQ=255)
                                                                                          gggCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCg                                                                                                       >  7:12131/1‑103 (MQ=255)
                                                                                            gCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGc                                                       >  8:194923/1‑149 (MQ=255)
                                                                                             cTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATATCGGCCTATCCGCAGGCGGTAACCGGCCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCt                                                      >  4:83022/1‑149 (MQ=255)
                                                                                              ttGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTg                                                     >  2:311041/1‑149 (MQ=255)
                                                                                                      cccACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCgg                                               >  7:56879/1‑147 (MQ=255)
                                                                                                           gCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGc                                        >  5:60246/1‑149 (MQ=255)
                                                                                                                aTCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGc                                    >  4:150264/1‑148 (MQ=255)
                                                                                                                   aGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGc                                <  6:343083/149‑1 (MQ=255)
                                                                                                                         ccATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGAc                          <  7:52656/149‑1 (MQ=255)
                                                                                                                          cATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACg                         >  3:194217/1‑149 (MQ=255)
                                                                                                                               ccATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTc                    <  3:329559/149‑1 (MQ=255)
                                                                                                                                      tGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTg              <  3:83022/148‑1 (MQ=255)
                                                                                                                                       ctcttcGACCACGCCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTggg            >  8:116034/6‑149 (MQ=255)
                                                                                                                                        tcttcGACCACGCCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTggg            >  8:264205/5‑148 (MQ=255)
                                                                                                                                         cGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCa          >  6:294153/1‑149 (MQ=255)
                                                                                                                                         cGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCa          >  3:319777/1‑149 (MQ=255)
                                                                                                                                                cACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGa   <  2:240651/149‑1 (MQ=255)
                                                                                                                                                cACGCCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGa   <  7:116034/149‑1 (MQ=255)
                                                                                                                                                 aCGCCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGAc  <  7:264205/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGGCCCGCGCCCGTCAGCAGCAACTGACCAAACCTGCTGGCTCGCTCGGCCAACTTGAAGCGCTGGCGATTCAGCTGGCTGGCCTTCAGGGGCTTGAACGACCCACGCTCGATCAGGTCGCCATCACCATTTTTGCCGGCGACCACGGCGTGGTCGAAGAGGGCATCTCGGCCTATCCGCAGGCGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGAC  >  NC_002947/1873747‑1874040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑