Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,915,602	T→A	9.2%	intergenic (+30/‑259)	fklB‑II → / → PP_1715	FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,915,602	0	T	A	9.2%	177.4 / 6.6	65	intergenic (+30/‑259)	fklB‑II/PP_1715	FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base A (3/3);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

GATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAA  >  NC_002947/1915455‑1915748                                                                                                                                                     |                                                                                                                                                   gATCCCGGCCAACTCGGTACTGGTTTTCGATCTGGAACTGATAGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCAGCAGGCCGAAGACACTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAtt                                                                                                                                                   >  7:117441/1‑149 (MQ=255)     ccGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAttgttg                                                                                                                                               >  4:180698/1‑149 (MQ=255)     ccGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAttgttg                                                                                                                                               >  7:171065/1‑149 (MQ=255)      cGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTgg                                                                                                                                              >  7:113398/1‑149 (MQ=255)         ccAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCAGCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTggg                                                                                                                                             <  7:293503/147‑1 (MQ=255)             cTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGttt                                                                                                                                       <  3:287060/149‑1 (MQ=255)             cTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGttt                                                                                                                                       >  3:169988/1‑149 (MQ=255)              tCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATAGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGtttt                                                                                                                                      >  3:7321/1‑149 (MQ=255)               cGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTc                                                                                                                                     >  5:28093/1‑149 (MQ=255)                ggTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGAAAACGCCCCAACAATG‑TGGGGCGtttcc                                                                                                                                     <  1:328552/148‑3 (MQ=255)                       gtgtTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgtg                                                                                                                              <  8:11472/148‑1 (MQ=255)                       gtgtTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgt                                                                                                                               <  5:346176/147‑1 (MQ=255)                                  ggAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACg                                                                                                                   >  6:99344/1‑148 (MQ=255)                                          tCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCaa                                                                                                          >  3:140912/1‑149 (MQ=255)                                          tCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCaa                                                                                                          <  4:263675/149‑1 (MQ=255)                                             ccATCAAGGACCCGGCCCAGTTGCACGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCTGAAACGACCC‑ACATTGTTTGGGCGTTTTCGTTTGTGCCGAtt                                                                                                                        >  8:150719/1‑132 (MQ=255)                                             ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCGC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAg                                                                                                       <  8:113398/149‑1 (MQ=255)                                             ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCCAACAATG‑TGGGGCGtt                                                                                                                                        >  3:123484/1‑116 (MQ=255)                                             ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCCAACAATG‑TGGGGCGtt                                                                                                                                        <  4:123484/116‑1 (MQ=255)                                              cATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGaaa                                                                                                          >  6:244408/1‑145 (MQ=255)                                                  aaGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGa                                                                                                   >  6:249643/1‑148 (MQ=255)                                                  aaGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGa                                                                                                   <  5:99344/148‑1 (MQ=255)                                                    ggACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAAc                                                                                                                >  6:177666/1‑133 (MQ=255)                                                    ggACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAAc                                                                                                                <  5:177666/133‑1 (MQ=255)                                                       cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                             <  6:28093/149‑1 (MQ=255)                                                       cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                             >  5:321249/1‑149 (MQ=255)                                                       cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                             >  4:106632/1‑149 (MQ=255)                                                       cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                             >  6:163683/1‑149 (MQ=255)                                                          ggCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTTGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTcc                                                                                           >  6:175158/1‑148 (MQ=255)                                                           gCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGGTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTcc                                                                                           >  1:336582/1‑147 (MQ=255)                                                            cccAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTTCAACAGCTGATACTGTCCGAg                                                                                        <  4:7321/149‑1 (MQ=255)                                                             ccAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAg                                                                                        >  6:326396/1‑148 (MQ=255)                                                              cAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAgg                                                                                       >  1:204032/1‑148 (MQ=255)                                                                   gCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGc                                                                                 >  3:195685/1‑149 (MQ=255)                                                                    cAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTTGGGCGTTTTCGTTTGTGCCGATTGTACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCa                                                                                <  7:40364/149‑1 (MQ=255)                                                                     aaGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAg                                                                               <  8:171065/149‑1 (MQ=255)                                                                     aaGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAg                                                                               <  4:195685/149‑1 (MQ=255)                                                                     aaGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACAATGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAg                                                                               <  8:117441/149‑1 (MQ=255)                                                                             gAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaag                                                                        >  4:292946/1‑148 (MQ=255)                                                                                ccGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGt                                                                    <  3:180698/149‑1 (MQ=255)                                                                                 cGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTa                                                                   <  5:326396/149‑1 (MQ=255)                                                                                 cGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTa                                                                   <  5:244408/149‑1 (MQ=255)                                                                                            gCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCt                                                         >  5:174873/1‑148 (MQ=255)                                                                                                cTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGc                                                    <  3:106632/149‑1 (MQ=255)                                                                                                    agaagCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCaaa                                                 <  6:174873/148‑1 (MQ=255)                                                                                                     gaagCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATc                                               <  4:169988/149‑1 (MQ=255)                                                                                                             tGCCAAGTAACATTGGCTGGATGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTCAAGAAGTAGGATCTTTCTGACGCAAATCGTTTgcgc                                       >  5:95853/1‑149 (MQ=255)                                                                                                               ccAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTgcgc                                       <  5:249643/147‑1 (MQ=255)                                                                                                               ccAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGcgccg                                     >  5:373501/1‑149 (MQ=255)                                                                                                                 aaGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaagaa                                                                      <  4:320287/114‑1 (MQ=255)                                                                                                                 aaGTAACATTGGATGGTTGTCGGAAAAGCCAC‑ACATTGTTGGGGAGATTTCGTTTGAGCCGATTGGAAGAACATGCAACAGCATATACTGTCCGAGGAAATGCAGCGTGAACAAGTAGAATCTTTCTGAAGCAAATCGTTTGcgccg                                     >  6:240406/1‑147 (MQ=255)                                                                                                                    tAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAACGATTTgcgtcagaaa                                 >  1:114944/1‑141 (MQ=255)                                                                                                                            gatGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGTACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGAAGCAAATCGTTTGAGCCGCCGGTAGTCAttg                        >  3:44870/3‑147 (MQ=255)                                                                                                                             cTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGc                       >  3:91518/1‑149 (MQ=255)                                                                                                                                gTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGt                     >  1:232343/1‑148 (MQ=255)                                                                                                                                 ttGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGa                   <  5:11735/149‑1 (MQ=255)                                                                                                                                      ggAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGa              >  7:221670/1‑149 (MQ=255)                                                                                                                                       gAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGt                                                                    <  6:3029/94‑1 (MQ=255)                                                                                                                                       gAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGt                                                                    >  5:3029/1‑94 (MQ=255)                                                                                                                                       gAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGAc             >  5:232262/1‑149 (MQ=255)                                                                                                                                       aaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGAGGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCa                           >  1:190572/2‑135 (MQ=255)                                                                                                                                        aaaCGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACt            <  5:175158/149‑1 (MQ=255)                                                                                                                                        aaaCGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACt            <  8:221670/149‑1 (MQ=255)                                                                                                                                          aCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGAc             <  6:332532/146‑1 (MQ=255)                                                                                                                                          aCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGAc             >  5:332532/1‑146 (MQ=255)                                                                                                                                                c‑acaTTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTaaaa    <  5:163683/149‑1 (MQ=255)                                                                                                                                                   caTTGTTGGGGCGTTTTCTTTTGTTCCGATTGGACTAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAATAAGTAGGATGTTTCTGACTCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTaaaaaa  <  2:336582/149‑1 (MQ=255)                                                                                                                                                   caTTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTaaaaaa  >  8:128910/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAA  >  NC_002947/1915455‑1915748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑