Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,927,034	G→A	7.2%	intergenic (‑169/‑159)	PP_1726 ← / → PP_1727	ABC transporter substrate‑binding protein/GntR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,927,034	0	G	A	7.2%	158.5 / 2.6	56	intergenic (‑169/‑159)	PP_1726/PP_1727	ABC transporter substrate‑binding protein/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base A (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

GCCTTTGCGATCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGAT  >  NC_002947/1926887‑1927179                                                                                                                                                     |                                                                                                                                                  gCCTTTGCGATCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGAGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCACTGAAGCTACAGCg                                                                                                                                                   >  7:343674/1‑148 (MQ=255)  ccTTTGCGATCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTa                                                                                                                                                 <  8:28747/149‑1 (MQ=255)   cTTTGCGATCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCg                                                                                                                                                   >  2:29740/1‑146 (MQ=255)      tGCGATCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGAc                                                                                                                                              >  5:109235/1‑148 (MQ=255)         gATCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAGCCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAgg                                                                                                                                          >  6:319891/1‑149 (MQ=255)          aTCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGCCCAgg                                                                                                                                          >  5:299860/1‑148 (MQ=255)                 ttCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGTCCGC‑TTGCGTGACAGTTTGATGTAGGGATGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGcc                                                                                                                                  >  3:232563/1‑149 (MQ=255)                  tCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGccc                                                                                                                                 >  1:308825/1‑149 (MQ=255)                    tGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCATAGACCAGGTAATCGCCCCg                                                                                                                               >  5:93405/1‑149 (MQ=255)                     gggTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGACGCTACAGCGTAGACCAGGTAATCGCCCCgg                                                                                                                              >  2:26658/1‑149 (MQ=255)                     gggTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCgg                                                                                                                              >  1:368778/1‑149 (MQ=255)                           aGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTctgc                                                                                                                        <  2:362708/149‑1 (MQ=255)                             ccGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTATAGCGTAGACCAGGTAATCGCCCCGGTTctgctg                                                                                                                      <  1:217101/149‑1 (MQ=255)                                  gCAAGCCTTTGACTGTCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCtt                                                                                                                  <  5:188442/148‑1 (MQ=255)                                        cTTTGACTGCCACCTTAGGTCTAGGTCAGCAAACCATGAGCCAAGGTAGGCCGC‑TTGCAAGACAGTTTGATGTAGGTAGGGGGCAGGCAAGCGCACAGAACCTACAGCCTTCACCAGGTAATCGACCAGGTTCTGCTGGCTTGGCTCGt                                                                                                           >  4:58320/1‑149 (MQ=255)                                            gACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGACAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCt                                                                                                       >  5:2904/1‑149 (MQ=255)                                              cTGGCACCTTTGGTCTAGGTCAGCAACCCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTgg                                                                                                     >  6:194894/1‑149 (MQ=255)                                               tGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGCATTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTgg                                                                                                     >  1:81239/1‑149 (MQ=255)                                               tGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCTCCCCGGTTCTGCTGGCTTGGCGCGTTTCTgg                                                                                                     >  3:219975/1‑148 (MQ=255)                                               tGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTgg                                                                                                     >  8:149588/1‑148 (MQ=255)                                                 gCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAg                                                                                                  <  2:368778/149‑1 (MQ=255)                                                    ccTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCATAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGc                                                                                               >  1:253850/1‑149 (MQ=255)                                                          gTCTAGGTCAGCATACCTTGAGCCAAGGTAGGGCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGc                                                                                          <  6:2904/148‑1 (MQ=255)                                                          gTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCATAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGc                                                                                          <  2:253850/148‑1 (MQ=255)                                                             tAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGccc                                                                                        >  6:194834/1‑147 (MQ=255)                                                             tAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGccc                                                                                        >  6:194839/1‑147 (MQ=255)                                                               gtgcAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCATGTAATAGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCATGGCCCAAg                                                                                     <  4:232563/145‑1 (MQ=255)                                                                 tCAGCTAAAGTTGAGCCATGGTAGTCCGC‑TTGCGTGAGAGTTTGATGTTGGGAGGGGGCCGGCAAGGGCCCTGAAGGTACAGCGTAGACCAGGTAATCTCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCtt                                                                                  <  8:343674/149‑1 (MQ=255)                                                                   aGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCttt                                                                                 <  5:194894/148‑1 (MQ=255)                                                                   aGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCttt                                                                                 <  6:109235/148‑1 (MQ=255)                                                                    gCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGt                                                                               <  7:98497/149‑1 (MQ=255)                                                                     cAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGt                                                                               >  5:132230/1‑148 (MQ=255)                                                                        aCCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCTGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCTCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTcaca                                                                           <  2:81239/149‑1 (MQ=255)                                                                          cTTGAGACAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTCGTCACAgg                                                                         >  8:77407/1‑149 (MQ=255)                                                                                         ggacgattGCGTGACAGTTTGATGTAGGGATGGGGCCGTCAATGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGGTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGcc                                                            <  8:87777/142‑1 (MQ=255)                                                                                          ccGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTg                                                         >  2:47068/1‑149 (MQ=255)                                                                                                  cGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGc                                                  <  5:51553/149‑1 (MQ=255)                                                                                                  cGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTCTCACAGGATGTTCATCAGCCCTGCATAGGc                                                  <  1:29740/149‑1 (MQ=255)                                                                                                   gTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCt                                                 >  8:282555/1‑149 (MQ=255)                                                                                                    tGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt                                                >  4:207365/1‑149 (MQ=255)                                                                                                     gACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGAGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTg                                               >  1:252436/1‑149 (MQ=255)                                                                                                      aCAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGGGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTg                                               >  2:306834/1‑148 (MQ=255)                                                                                                      aCAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTg                                               <  5:319891/148‑1 (MQ=255)                                                                                                       cAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCa                                             <  6:132230/149‑1 (MQ=255)                                                                                                                   gggagggGGCCGGCAAGGTCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAAc                                  <  5:9521/148‑1 (MQ=255)                                                                                                                   gggagggGGCCGGCAAGGGCCGTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAAc                                  <  3:58320/148‑1 (MQ=255)                                                                                                                      agggGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCATCCCTGCATAGGCTTGCACTATTCTCAACATGc                              <  5:194834/149‑1 (MQ=255)                                                                                                                      agggGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATCTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGc                              <  5:194839/149‑1 (MQ=255)                                                                                                                      agggGGCCGGCAAGGGCCCTGAAGCTACAGCATAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGACAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGc                              <  6:93405/149‑1 (MQ=255)                                                                                                                         gggCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTca                                                                             >  7:349145/1‑99 (MQ=255)                                                                                                                         gggCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTca                                                                             <  8:349145/99‑1 (MQ=255)                                                                                                                         gggCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCt                           <  4:219975/149‑1 (MQ=255)                                                                                                                              ggCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGAt                      <  3:207365/149‑1 (MQ=255)                                                                                                                                 aaGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATggg                   <  2:308825/149‑1 (MQ=255)                                                                                                                                   gggCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGc                 >  8:51964/1‑149 (MQ=255)                                                                                                                                             gCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTc       >  8:18398/1‑149 (MQ=255)                                                                                                                                             gCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTc       <  1:47068/149‑1 (MQ=255)                                                                                                                                             gCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTCGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTc       <  7:77407/149‑1 (MQ=255)                                                                                                                                                     gTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGAt  >  2:282047/1‑146 (MQ=255)                                                                                                                                                     gTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGAt  <  1:282047/146‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCCTTTGCGATCGTTGTTCTGGGTAAAGCCGTTGCAAGCCTTTGACTGGCACCTTTGGTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGC‑TTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGAT  >  NC_002947/1926887‑1927179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑