Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,025,124	C→T	23.4%	L42F (CTC→TTC)	wbpZ →	glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,025,124	0	C	T	23.4%	117.0 / 35.4	70	L42F (CTC→TTC)	wbpZ	glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base T (8/8);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-02

TTTACAAGTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGCCCAGGCCGACATTGTGCA  >  NC_002947/2024979‑2025272                                                                                                                                                    |                                                                                                                                                     tttACAAGTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCa                                                                                                                                                   <  1:90250/149‑1 (MQ=255)  ttACAAGTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAg                                                                                                                                                  >  3:47603/1‑149 (MQ=255)  ttACAAGTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAg                                                                                                                                                  >  8:18742/1‑149 (MQ=255)    aCAAGTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGcc                                                                                                                                                >  1:329032/1‑149 (MQ=255)      aaGTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAAGAAATATTTAAAAAGCGAGCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACATCAGcc                                                                                                                                                >  1:308024/1‑149 (MQ=255)        gTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCCCCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACAGCAGCCcgcg                                                                                                                                            >  6:278729/1‑149 (MQ=255)        gTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCcgcg                                                                                                                                            >  5:109246/1‑149 (MQ=255)         tCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGt                                                                                                                                           <  7:116064/149‑1 (MQ=255)            gTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTggg                                                                                                                                        >  7:20913/1‑149 (MQ=255)               aaGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCt                                                                                                                                     >  6:313912/1‑149 (MQ=255)                  gAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTggg                                                                                                                                        >  1:264355/1‑143 (MQ=255)                  gAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTggg                                                                                                                                        <  2:264355/143‑1 (MQ=255)                  gAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTggg                                                                                                                                        <  2:259240/143‑1 (MQ=255)                  gAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTggg                                                                                                                                        >  1:259240/1‑143 (MQ=255)                  gAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTgcg                                                                                                                                  >  5:266159/1‑149 (MQ=255)                   aaCGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTgcgc                                                                                                                                 >  2:95390/1‑149 (MQ=255)                       atgatCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCaa                                                                                                                             <  3:31869/149‑1 (MQ=255)                           tCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGaa                                                                                                                         >  8:111809/1‑149 (MQ=255)                             aaGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTCTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAAc                                                                                                                        <  6:235743/148‑1 (MQ=255)                              aGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCg                                                                                                                      <  2:329032/149‑1 (MQ=255)                                gTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTc                                                                                                                                                    <  8:66152/117‑1 (MQ=255)                                gTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTc                                                                                                                                                    >  7:66152/1‑117 (MQ=255)                                 ttCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTa                                                                                                                   <  5:101883/149‑1 (MQ=255)                                   cTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAgg                                                                                                                 >  6:287655/1‑149 (MQ=255)                                     gCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAAt                                                                                                                            <  2:3488/136‑1 (MQ=255)                                     gCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAAt                                                                                                                            >  1:3488/1‑136 (MQ=255)                                      cATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAgg                                                                                                                 <  5:313912/146‑1 (MQ=255)                                             ttAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCt                                                                                                        >  6:129631/1‑148 (MQ=255)                                             ttAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCt                                                                                                        <  7:111809/148‑1 (MQ=255)                                              tAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCaa                                                                                                                             <  8:88051/126‑1 (MQ=255)                                                aaaCGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCt                                                                                                                                     <  1:36449/116‑1 (MQ=255)                                                aaaCGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCt                                                                                                                                     >  2:36449/1‑116 (MQ=255)                                                   cGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCAc                                                                                                 <  5:129631/149‑1 (MQ=255)                                                     tATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCg                                                                                               >  3:305258/1‑149 (MQ=255)                                                          cccGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTcc                                                                                           >  6:204404/1‑148 (MQ=255)                                                                  ccATGGGGGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGAc                                                                                  <  8:200435/149‑1 (MQ=255)                                                                   cATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGAc                                                                                  <  4:205028/148‑1 (MQ=255)                                                                    aTGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGTAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACtt                                                                                >  1:65666/1‑149 (MQ=255)                                                                    aTGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACtt                                                                                >  8:354272/1‑149 (MQ=255)                                                                     tGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTg                                                                               >  3:7256/1‑149 (MQ=255)                                                                        gTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCAt                                                                            >  2:161437/1‑149 (MQ=255)                                                                                 aaCAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGCCTCACCGCTCCAAGCTGGACTTGCATGTCGCTTc                                                                    >  6:15916/1‑148 (MQ=255)                                                                                  aCAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCaa                                                                  >  3:90148/1‑149 (MQ=255)                                                                                   cAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCAGGTCGCTTCAAc                                                                 >  3:271057/1‑149 (MQ=255)                                                                                     ggTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACgg                                                               <  5:204404/149‑1 (MQ=255)                                                                                          t‑ttttCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGc                                                                              >  7:45861/1‑129 (MQ=255)                                                                                          t‑ttttCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGc                                                                              <  8:45861/129‑1 (MQ=255)                                                                                              ttCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGTGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTc                                                        <  2:125150/148‑1 (MQ=255)                                                                                              ttCAGATCG‑CCGAAGGCACCCGGGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTc                                                        <  8:179945/148‑1 (MQ=255)                                                                                               tCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCa                                                       <  6:109246/148‑1 (MQ=255)                                                                                               tCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCCGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCa                                                       <  1:95390/148‑1 (MQ=255)                                                                                                   aTCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTc                                                  >  2:9496/1‑149 (MQ=255)                                                                                                   aTCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTc                                                  >  4:340149/1‑149 (MQ=255)                                                                                                   aTCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTc                                                  <  5:278729/149‑1 (MQ=255)                                                                                                        ccGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTg                                              <  5:287655/149‑1 (MQ=255)                                                                                                             ggCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCaa                                         <  4:7256/149‑1 (MQ=255)                                                                                                              gCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAg                                        <  7:18742/149‑1 (MQ=255)                                                                                                                     tGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCg                                 >  8:189204/1‑149 (MQ=255)                                                                                                                         cACGGCGTGCAATCAGAGGTGCTATACTTCCGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGa                             <  4:47603/149‑1 (MQ=255)                                                                                                                         cACGGCGTGCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGa                             <  2:65666/149‑1 (MQ=255)                                                                                                                         cACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGa                             <  7:82507/149‑1 (MQ=255)                                                                                                                                 gCAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTggcgg                     >  2:343666/1‑149 (MQ=255)                                                                                                                                  cAATCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTggcggc                    <  1:343666/149‑1 (MQ=255)                                                                                                                                    aTCAGAGGTGCTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGcc                   >  2:259520/1‑148 (MQ=255)                                                                                                                                       agagGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGCCCAgg               >  1:20633/1‑149 (MQ=255)                                                                                                                                              cTATACTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGCCCAGGCCGACAt        >  2:184666/1‑149 (MQ=255)                                                                                                                                              cTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGCCTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGccc                  <  6:69858/139‑1 (MQ=255)                                                                                                                                              cTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAACGATTTCGCCGAGCTGGCGGccc                  >  5:69858/1‑139 (MQ=255)                                                                                                                                                 taCTTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGCCCAGGCCGACAtt       <  1:184666/147‑1 (MQ=255)                                                                                                                                                 taCCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGCCCAGGCCGACAtt       <  7:354272/147‑1 (MQ=255)                                                                                                                                                    cTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGCCCAGGCCGACATTGTGCa  >  5:313773/1‑149 (MQ=255)                                                                                                                                                    cTCAGCCAGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACTGGTTTTTCACTGTCGGTGATCAAGGATTTCGACGAGCTGGCGGCCCAGGCCGACATTGTGCa  >  4:300324/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TTTACAAGTCGGTCAAGGAACGATGATCAAGGTTCTGCATTTTTTTAAAACGTATTACCCGGACTCCATGGGTGGCATCGAACAGGTCAT‑TTTTCAGATCG‑CCGAAGGCACCCGTGAGCACGGCGTGCAATCAGAGGTGCTATACCTCAGCCCGCGTGGGGCTGCGCGCAATGAACCGGTAGGCAGCCACCTGACTCACCGCTCCAAGCTGGACTTGCATGTCGCTTCAACGGGTTTTTCACTGTCGGTGATCAAGGATTTCGCCGAGCTGGCGGCCCAGGCCGACATTGTGCA  >  NC_002947/2024979‑2025272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑