Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,026,908 C→T 8.7% I222I (ATC→ATT)  wbpV → UDP‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,026,9080CT 8.7% 126.5 / 3.0 46I222I (ATC→ATT) wbpVUDP‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base C (19/23);  new base T (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GTCTACGGGCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTGTCCCCGCGGGCCTGTTGCGCGGCGCT  >  NC_002947/2026765‑2027052
                                                                                                                                               |                                                                                                                                                
gTCTACGGGCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGat                                                                                                                                               <  2:86964/147‑1 (MQ=255)
gTCTACGGGCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTAACCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGatca                                                                                                                                             >  7:245423/1‑149 (MQ=255)
     cGGGCCAGGCGTCAAGGCCAATTTCCACAGTTTGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGat                                                                                                                                               >  6:226115/1‑142 (MQ=255)
     cGGGCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCGGTTCGGCGCGGTTTGCAACCGTCTCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTCCATCGATCATCCTc                                                                                                                                        <  7:320142/149‑1 (MQ=255)
     cGGGCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGat                                                                                                                                               <  5:226115/142‑1 (MQ=255)
     cGGGCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTc                                                                                                                                        >  7:104284/1‑149 (MQ=255)
      gggCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTcg                                                                                                                                       <  7:160531/149‑1 (MQ=255)
           aGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTg                                                                                                                                  >  7:216717/1‑149 (MQ=255)
            ggCGTAAAGGCCAATTTCCACAGTCTGATGCCCTGGCTGCAGCGTGGCGTAGCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGc                                                                                                                                 <  4:29301/149‑1 (MQ=255)
               gTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGccaa                                                                                                                              <  3:321508/149‑1 (MQ=255)
                  aaGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGccaacc                                                                                                                            <  5:8936/148‑1 (MQ=255)
                  aaGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGccaacc                                                                                                                            >  5:203381/1‑148 (MQ=255)
                   aGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATTGATCATCCTCGCGCTGccaacca                                                                                                                           >  8:344839/1‑148 (MQ=255)
                    ggCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCaaa                                                                                                                         <  3:111506/149‑1 (MQ=255)
                    ggCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCaaa                                                                                                                         <  8:216717/149‑1 (MQ=255)
                     gCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAAc                                                                                                                        >  1:27913/1‑149 (MQ=255)
                         aTTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTc                                                                                                                    <  5:258799/149‑1 (MQ=255)
                              cacaGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGc                                                                                                               >  2:311323/1‑149 (MQ=255)
                                   tATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGGCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGc                                                                                                           >  7:196568/1‑148 (MQ=255)
                                                    aGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCg                                                                                         <  5:137146/149‑1 (MQ=255)
                                                     gCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGc                                                                                        >  6:228312/1‑149 (MQ=255)
                                                               cccTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGGCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAAc                                                                               <  1:352527/148‑1 (MQ=255)
                                                                    gCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCt                                                                           <  5:324678/147‑1 (MQ=255)
                                                                      cGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGt                                                                       >  2:247513/1‑149 (MQ=255)
                                                                             cgcgGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTgg                                                                >  3:347583/1‑149 (MQ=255)
                                                                             cgcgGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTgg                                                                <  8:182516/149‑1 (MQ=255)
                                                                                 gTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATTGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCt                                                            <  7:344839/149‑1 (MQ=255)
                                                                                    tGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTAGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTg                                                          <  5:264564/148‑1 (MQ=255)
                                                                                     gCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTgc                                                         <  8:104284/148‑1 (MQ=255)
                                                                                         ccGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCg                                                    <  4:347583/149‑1 (MQ=255)
                                                                                          cGTCGCAGCTTGGTCTCGTTGGCTAACCTGGGCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCgg                                                   <  8:196568/149‑1 (MQ=255)
                                                                                                      gTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTc                                        <  1:247513/148‑1 (MQ=255)
                                                                                                           gTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGt                                  >  1:194340/1‑149 (MQ=255)
                                                                                                           gTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGt                                  >  7:249137/1‑149 (MQ=255)
                                                                                                            ttGGCTAACCTCGGCGACCTGGCCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCACACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCGCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGtt                                 <  8:245423/149‑1 (MQ=255)
                                                                                                                cTAACCTGGTCGACCTGGTCGTTACCTGCATTGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCt                             >  6:87336/1‑149 (MQ=255)
                                                                                                                cTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCt                             >  2:59207/1‑149 (MQ=255)
                                                                                                                 tAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTg                            >  4:146242/1‑149 (MQ=255)
                                                                                                                   acctggtcgacctggtcgTTACCTGCATTGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTg                            <  5:87336/147‑1 (MQ=255)
                                                                                                                     ctggtcgacctggtcgTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTGTccc                        >  4:193254/1‑149 (MQ=255)
                                                                                                                              ctggtcgTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTGTCCCCGCGGGCCt               <  1:311323/149‑1 (MQ=255)
                                                                                                                                ggtcgTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCGGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGtt                                 >  2:9991/1‑129 (MQ=255)
                                                                                                                                    gTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTGTCCCCGCGGGCCTGTTgcg         >  2:43194/1‑149 (MQ=255)
                                                                                                                                         cTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGggcgg                                               >  4:149785/1‑106 (MQ=255)
                                                                                                                                         cTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGggcgg                                               <  3:149785/106‑1 (MQ=255)
                                                                                                                                            cATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTGTCCCCGCGGGCCTGTTGCGCGGCgct  <  1:43194/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GTCTACGGGCCAGGCGTCAAGGCCAATTTCCACAGTATGATGCGCTGGCTGCAGCGTGGCGTACCCTTGCCGTTCGGCGCGGTTTGCAACCGTCGCAGCTTGGTCTCGTTGGCTAACCTGGTCGACCTGGTCGTTACCTGCATCGATCATCCTCGCGCTGCCAACCAAACCTTCCTGGCCAGCGACGGTGACGATGTCTCGCTCACCCAACTGCTACGTGCACTGGGCCTTGCGCTCGGGCGGCCCGCTCGGCTGTTGCCTGTCCCCGCGGGCCTGTTGCGCGGCGCT  >  NC_002947/2026765‑2027052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑