Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,042,440 A→T 8.1% L39Q (CTG→CAG)  PP_1816 ← alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,042,4400AT 8.1% 218.2 / 7.4 74L39Q (CTG→CAG) PP_1816alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (35/33);  new base T (4/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

GTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAGGGCGTAAG  >  NC_002947/2042295‑2042587
                                                                                                                                                  |                                                                                                                                                   
gTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGt                                                                                                                                                   <  6:82972/148‑1 (MQ=255)
gTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGt                                                                                                                                                   <  6:77637/148‑1 (MQ=255)
 tAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTAc                                                                                                                                                 >  2:316940/1‑149 (MQ=255)
 tAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTAc                                                                                                                                                 >  3:133097/1‑149 (MQ=255)
            aTTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGc                                                                                                                                      >  8:241268/1‑149 (MQ=255)
                gAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCggg                                                                                                                                  <  4:232574/149‑1 (MQ=255)
                  ggCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgtt                                                                                                                                <  4:66094/149‑1 (MQ=255)
                   gCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgttg                                                                                                                               >  3:212053/1‑149 (MQ=255)
                     aaCCACTTCACCTACACGCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgttgtt                                                                                                                             <  8:83538/149‑1 (MQ=255)
                     aaCCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGgttgtt                                                                                                                             >  4:174301/1‑149 (MQ=255)
                     aaCCACTTCACCTACACCCAGCGCACGCATTACATGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATCGCCGGGTCCAGCGACAGGTACAGGATGCGC‑ACCAGTACCTGGCCTTCGCCGGgttgtg                                                                                                                             >  7:79498/1‑148 (MQ=255)
                             cACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCggg                                                                                                                      <  7:47073/148‑1 (MQ=255)
                                cTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACg                                                                                                                  <  4:133097/149‑1 (MQ=255)
                                     cccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGtctc                                                                                                             >  4:25848/1‑149 (MQ=255)
                                      ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCgg                                                                                                                                   >  5:20139/1‑126 (MQ=255)
                                      ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCgg                                                                                                                                   <  6:20139/126‑1 (MQ=255)
                                      ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGtctc                                                                                                             >  3:52964/1‑148 (MQ=255)
                                      ccAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGtctc                                                                                                             >  4:314309/1‑148 (MQ=255)
                                        aGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGaa                                                                                                          <  7:100346/149‑1 (MQ=255)
                                         gcgcACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCCGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAg                                                                                                         >  2:51417/1‑149 (MQ=255)
                                          cgcacgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGc                                                                                                        >  3:132699/1‑149 (MQ=255)
                                           gcacgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCt                                                                                                       >  3:212690/1‑149 (MQ=255)
                                             acgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTGGCCTTCGTTGATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACATGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGa                                                                                                     <  8:327608/149‑1 (MQ=255)
                                              cgcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGaa                                                                                                    <  4:364583/149‑1 (MQ=255)
                                               gcaTTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAg                                                                                                   >  5:235793/1‑149 (MQ=255)
                                                    aCCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAgtcgt                                                                                               <  1:316940/148‑1 (MQ=255)
                                                     ccTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAACCTGAAgtcgtcc                                                                                             >  7:195017/1‑148 (MQ=255)
                                                      cTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGTGGGTACGGTCTCGAAGCTGAAGTCGTcac                                                                                            >  5:334644/1‑149 (MQ=255)
                                                      cTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcac                                                                                            >  5:366771/1‑149 (MQ=255)
                                                      cTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcac                                                                                            >  5:124793/1‑149 (MQ=255)
                                                        ggCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacgg                                                                                          >  5:218337/1‑149 (MQ=255)
                                                         gCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggc                                                                                         >  1:210244/1‑149 (MQ=255)
                                                          cccAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggcg                                                                                        >  7:47081/1‑149 (MQ=255)
                                                           ccAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggcgc                                                                                       >  2:78417/1‑149 (MQ=255)
                                                            cAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTcacggcgca                                                                                      >  6:42479/1‑149 (MQ=255)
                                                                   aCGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGcc                                                                               <  8:47081/149‑1 (MQ=255)
                                                                       gcgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtga                                                                           <  3:314309/149‑4 (MQ=255)
                                                                       gcgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtga                                                                           <  1:78417/149‑4 (MQ=255)
                                                                       gcgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtga                                                                           <  7:241268/149‑4 (MQ=255)
                                                                        cgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtgac                                                                          <  4:52964/149‑5 (MQ=255)
                                                                        cgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCCGTGCGCCgtgac                                                                          <  4:105117/149‑5 (MQ=255)
                                                                        cgggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCg                                                                          <  6:235793/149‑1 (MQ=255)
                                                                         gggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCgg                                                                         >  6:207724/1‑149 (MQ=255)
                                                                          ggATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCggg                                                                        <  4:132699/149‑1 (MQ=255)
                                                                           gATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGc                                                                       <  6:218337/149‑1 (MQ=255)
                                                                               tAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGttt                                                                   <  3:174301/149‑1 (MQ=255)
                                                                                 ggACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTg                                                                  <  6:124793/148‑1 (MQ=255)
                                                                                  gACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTgg                                                                 >  4:288124/1‑148 (MQ=255)
                                                                                  gACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTgg                                                                 >  7:36198/1‑148 (MQ=255)
                                                                                   aCTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTTCGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCTGGCGTTTGGCa                                                               <  4:212053/149‑1 (MQ=255)
                                                                                         cTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAgg                                                         <  6:334644/149‑1 (MQ=255)
                                                                                             gTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAg                                                      <  4:212690/148‑1 (MQ=255)
                                                                                                cATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCCGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAgcgg                                                   <  1:51417/148‑1 (MQ=255)
                                                                                                 aTCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGa                                                                             <  8:306196/121‑1 (MQ=255)
                                                                                                 aTCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGa                                                                             >  7:306196/1‑121 (MQ=255)
                                                                                                  tCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGcggcgg                                                <  5:207724/149‑1 (MQ=255)
                                                                                                     aGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTg                                             <  3:25848/149‑1 (MQ=255)
                                                                                                              tGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTc                                    <  6:366771/149‑1 (MQ=255)
                                                                                                                     gTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCgg                             <  8:36198/149‑1 (MQ=255)
                                                                                                                        cAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTc                          >  3:221943/1‑149 (MQ=255)
                                                                                                                        cAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGCCGGTTGGTCTGGTTCATGTCGGGTc                          >  2:36533/1‑149 (MQ=255)
                                                                                                                         aGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTcc                         >  8:166571/1‑149 (MQ=255)
                                                                                                                         aGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTcc                         >  3:202129/1‑149 (MQ=255)
                                                                                                                           ggccaggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGTGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCtt                       >  8:330972/4‑149 (MQ=255)
                                                                                                                           ggccaggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCtt                       >  5:129859/4‑149 (MQ=255)
                                                                                                                              caggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTgg                                       <  1:368496/130‑1 (MQ=255)
                                                                                                                              caggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTgg                                       >  2:368496/1‑130 (MQ=255)
                                                                                                                              caggtactgg‑tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGCCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTtgtg                    >  8:111326/1‑149 (MQ=255)
                                                                                                                                   acaggtTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCgg               >  8:60123/1‑149 (MQ=255)
                                                                                                                                     aggtTGCGC‑AGCAGTTCCTGGCCTGCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTg             <  8:195017/149‑1 (MQ=255)
                                                                                                                                       gtTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTg             >  2:136529/1‑147 (MQ=255)
                                                                                                                                         tGCGCAACCTGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAg          <  6:129859/149‑1 (MQ=255)
                                                                                                                                               aCCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAGGGCGt     >  2:10744/1‑148 (MQ=255)
                                                                                                                                                 cAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAGGGCGTAAg  >  7:46048/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTAGCCGGGGTGATTGGAGGCAACCACTTCACCTACACCCAGCGCACGCATTACCTGGCCCAGGGCCACGGGCGGGATGTAGGACTTGCCTTCGTTCATCCAGCCGCGCATGGCCGGGTCCAGCGACAGGTACAGGTTGCGC‑ACCAGTACCTGGCCTTCGCCGGGTTGTTCGGCGGGTACGGTCTCGAAGCTGAAGTCGTCACGGCGCACGGCGCCGACCGGGCGTTTGGCAAGCAGGAAGCGGCGGTTGGTCTGGGTCATGGCGGGTCCTTGTGGGCGGTGGAGGGCGTAAG  >  NC_002947/2042295‑2042587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑