Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,045,670 T→G 7.4% I295S (ATC→AGC)  PP_1819 → methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,045,6700TG 7.4% 161.6 / 4.8 54I295S (ATC→AGC) PP_1819methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base T (22/28);  new base G (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TCAACGAATACACCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCG  >  NC_002947/2045526‑2045818
                                                                                                                                                |                                                                                                                                                     
tCAACGAATACACCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATgccatcgc                                                                                                                                                    >  8:239732/1‑148 (MQ=255)
tCAACGAATACACCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATgccatcgc                                                                                                                                                    >  5:66322/1‑148 (MQ=255)
   aCGAATAGACCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGTAGTGTCGACCAAGGCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATgccatcgccatc                                                                                                                                                <  3:294317/149‑1 (MQ=255)
    cGAATACACCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGccatcgccatcg                                                                                                                                               <  1:246738/149‑1 (MQ=255)
          cacCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTgg                                                                                                                                         <  2:42097/149‑1 (MQ=255)
           acCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGc                                                                                                                                        >  5:209266/1‑149 (MQ=255)
           acCGACCAGCTGGCCAGCGAGCACGGCCTGAAGGCGCAGTTGCAAGCCAGCGCCCAGCGGGTAAACGTGAGTGTCGACAAAGACTAACTTGGGCAGGAGCACGCCCTGCAAGGAGACCAGCAGCGCAATGCCATCGCAATCGGCCTGGc                                                                                                                                        >  4:324450/1‑149 (MQ=255)
                      ggCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTgg                                                                                                                            >  5:88332/1‑149 (MQ=255)
                       gCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTg                                                                                                                             <  1:181651/147‑1 (MQ=255)
                        cccGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCg                                                                                                                          <  6:88332/149‑1 (MQ=255)
                            cgAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                      <  4:310146/149‑1 (MQ=255)
                            cgAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                      <  4:32314/149‑1 (MQ=255)
                            cgAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                      <  4:340172/149‑1 (MQ=255)
                                   gggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCgg                                                                                                               >  1:200142/1‑149 (MQ=255)
                                   gggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAACGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTg                                                                                                                       >  6:359313/1‑141 (MQ=37)
                                   gggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAACGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTg                                                                                                                       <  5:359313/141‑1 (MQ=37)
                                    ggCTGCAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGc                                                                                                              >  4:278451/1‑149 (MQ=255)
                                    ggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGc                                                                                                              <  7:97516/149‑1 (MQ=255)
                                              gcAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTggcgg                                                                                                    >  7:15342/1‑149 (MQ=255)
                                                  ttGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTg                                                                                                >  6:86639/1‑149 (MQ=255)
                                                         ccAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTGcg                                                                                                                     <  7:325860/121‑2 (MQ=25)
                                                         ccAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTGcg                                                                                                                     >  8:325860/1‑120 (MQ=25)
                                                           aGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTAc                                                                                       >  6:231731/1‑149 (MQ=255)
                                                             cgcCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTAccc                                                                                     <  2:307884/149‑1 (MQ=255)
                                                                   gCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCtg                                                                               <  5:231731/149‑1 (MQ=255)
                                                                          aCCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCg                                                                         <  2:200142/148‑1 (MQ=255)
                                                                           ccGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCgg                                                                        <  1:6086/148‑1 (MQ=255)
                                                                           ccGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGc                                                                       >  8:238061/1‑149 (MQ=255)
                                                                                cGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGtt                                                                  <  2:173925/149‑1 (MQ=255)
                                                                                   gtCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCg                                                                         >  1:63503/1‑139 (MQ=255)
                                                                                   gtCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCg                                                                         <  2:63503/139‑1 (MQ=255)
                                                                                    tCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                      <  1:178272/93‑1 (MQ=255)
                                                                                    tCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                      >  2:178272/1‑93 (MQ=255)
                                                                                    tCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGaa                                                               <  7:70251/148‑1 (MQ=255)
                                                                                      gACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCt                                                                                                 >  2:183338/1‑112 (MQ=255)
                                                                                       aCCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCAt                                                           >  5:328250/1‑149 (MQ=255)
                                                                                            gCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGAt                                                      <  6:328250/149‑1 (MQ=255)
                                                                                            gCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGAt                                                      <  6:66322/149‑1 (MQ=255)
                                                                                                aTCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGGTTGTCGGCGTGTTGGCGGCCTGGGTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCTGGTGATCGcc                                                  >  6:63399/1‑149 (MQ=255)
                                                                                                       gCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGccc                                           <  7:239732/149‑1 (MQ=255)
                                                                                                       gCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGcc                                            >  1:321583/1‑148 (MQ=255)
                                                                                                          ggAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAgc                                        <  6:209266/149‑1 (MQ=255)
                                                                                                              cAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCAt                                                           >  3:144544/1‑126 (MQ=255)
                                                                                                              cAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCAt                                                           <  4:144544/126‑1 (MQ=255)
                                                                                                              cAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCAt                                    <  3:278451/149‑1 (MQ=255)
                                                                                                                 gCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATc                                                     >  1:181438/1‑129 (MQ=255)
                                                                                                                 gCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATc                                                     <  2:181438/129‑1 (MQ=255)
                                                                                                                 ctccTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCAtt                                   <  5:268246/145‑1 (MQ=255)
                                                                                                                      gCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCTCCCGCGCCCAGCGCATTGcggcgg                            <  7:52665/149‑1 (MQ=255)
                                                                                                                      gCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGcggcgg                            <  5:63399/149‑1 (MQ=255)
                                                                                                                           gCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGAGTACCCGCGCTGTGGGCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCCGCGCATTGCGGCGGGTGaa                       >  4:208373/1‑149 (MQ=255)
                                                                                                                                  gCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTggc                                                                                                      <  7:159830/63‑1 (MQ=255)
                                                                                                                                  gCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTggc                                                                                                      >  8:159830/1‑63 (MQ=255)
                                                                                                                                   cAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCtt               >  8:161496/1‑149 (MQ=255)
                                                                                                                                    aGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTc              >  3:256623/1‑149 (MQ=255)
                                                                                                                                     gcgcAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTc              >  2:50978/1‑148 (MQ=255)
                                                                                                                                     gcgcAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCg             <  2:321583/149‑1 (MQ=255)
                                                                                                                                           tGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGccc       <  7:161496/149‑1 (MQ=255)
                                                                                                                                            gccatcgccatcGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGcc        >  5:29763/1‑147 (MQ=255)
                                                                                                                                               atcgccatcgGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTg                    >  1:201161/1‑132 (MQ=255)
                                                                                                                                               atcgccatcgGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTg                    <  2:201161/132‑1 (MQ=255)
                                                                                                                                                tcgccatcgGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGgcg  >  3:37525/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TCAACGAATACACCGACCAGCTGGCCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCG  >  NC_002947/2045526‑2045818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑