Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,045,698 G→A 6.1% A304A (GCG→GCA)  PP_1819 → methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,045,6980GA 6.1% 196.7 / 2.2 65A304A (GCG→GCA) PP_1819methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base A (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAGGTGGGGCAACT  >  NC_002947/2045550‑2045844
                                                                                                                                                     |                                                                                                                                                  
cccGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCg                                                                                                                                                    <  6:88332/149‑1 (MQ=255)
    cgAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                                                <  4:340172/149‑1 (MQ=255)
    cgAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                                                <  4:32314/149‑1 (MQ=255)
    cgAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                                                <  4:310146/149‑1 (MQ=255)
           gggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCgg                                                                                                                                         >  1:200142/1‑149 (MQ=255)
           gggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAACGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTg                                                                                                                                                 >  6:359313/1‑141 (MQ=37)
           gggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAACGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTg                                                                                                                                                 <  5:359313/141‑1 (MQ=37)
            ggCTGCAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGc                                                                                                                                        >  4:278451/1‑149 (MQ=255)
            ggCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGc                                                                                                                                        <  7:97516/149‑1 (MQ=255)
                      gcAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTggcgg                                                                                                                              >  7:15342/1‑149 (MQ=255)
                          ttGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTg                                                                                                                          >  6:86639/1‑149 (MQ=255)
                                 ccAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTGcg                                                                                                                                               >  8:325860/1‑120 (MQ=25)
                                 ccAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCAGCGCCGT‑GGCCAGGCCGATGGCGATGGCATTGcg                                                                                                                                               <  7:325860/121‑2 (MQ=25)
                                   aGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTAc                                                                                                                 >  6:231731/1‑149 (MQ=255)
                                     cgcCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTAccc                                                                                                               <  2:307884/149‑1 (MQ=255)
                                           gCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCtg                                                                                                         <  5:231731/149‑1 (MQ=255)
                                                  aCCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCg                                                                                                   <  2:200142/148‑1 (MQ=255)
                                                   ccGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCgg                                                                                                  <  1:6086/148‑1 (MQ=255)
                                                   ccGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGc                                                                                                 >  8:238061/1‑149 (MQ=255)
                                                        cGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGtt                                                                                            <  2:173925/149‑1 (MQ=255)
                                                           gtCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCg                                                                                                   <  2:63503/139‑1 (MQ=255)
                                                           gtCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCg                                                                                                   >  1:63503/1‑139 (MQ=255)
                                                            tCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                                                >  2:178272/1‑93 (MQ=255)
                                                            tCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGc                                                                                                                                                <  1:178272/93‑1 (MQ=255)
                                                            tCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGaa                                                                                         <  7:70251/148‑1 (MQ=255)
                                                              gACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCt                                                                                                                           >  2:183338/1‑112 (MQ=255)
                                                               aCCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCAt                                                                                     >  5:328250/1‑149 (MQ=255)
                                                                    gCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGAt                                                                                <  6:66322/149‑1 (MQ=255)
                                                                    gCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGAt                                                                                <  6:328250/149‑1 (MQ=255)
                                                                        aTCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGGTTGTCGGCGTGTTGGCGGCCTGGGTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCTGGTGATCGcc                                                                            >  6:63399/1‑149 (MQ=255)
                                                                               gCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGccc                                                                     <  7:239732/149‑1 (MQ=255)
                                                                               gCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGcc                                                                      >  1:321583/1‑148 (MQ=255)
                                                                                  ggAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAgc                                                                  <  6:209266/149‑1 (MQ=255)
                                                                                      cAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCAt                                                                                     <  4:144544/126‑1 (MQ=255)
                                                                                      cAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCAt                                                                                     >  3:144544/1‑126 (MQ=255)
                                                                                      cAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCAt                                                              <  3:278451/149‑1 (MQ=255)
                                                                                         gCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATc                                                                               >  1:181438/1‑129 (MQ=255)
                                                                                         gCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATc                                                                               <  2:181438/129‑1 (MQ=255)
                                                                                         ctccTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCAtt                                                             <  5:268246/145‑1 (MQ=255)
                                                                                              gCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCTCCCGCGCCCAGCGCATTGcggcgg                                                      <  7:52665/149‑1 (MQ=255)
                                                                                              gCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGcggcgg                                                      <  5:63399/149‑1 (MQ=255)
                                                                                                   gCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGAGTACCCGCGCTGTGGGCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCCGCGCATTGCGGCGGGTGaa                                                 >  4:208373/1‑149 (MQ=255)
                                                                                                          gCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTggc                                                                                                                                >  8:159830/1‑63 (MQ=255)
                                                                                                          gCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTggc                                                                                                                                <  7:159830/63‑1 (MQ=255)
                                                                                                           cAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCtt                                         >  8:161496/1‑149 (MQ=255)
                                                                                                            aGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTc                                        >  3:256623/1‑149 (MQ=255)
                                                                                                             gcgcAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTc                                        >  2:50978/1‑148 (MQ=255)
                                                                                                             gcgcAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCg                                       <  2:321583/149‑1 (MQ=255)
                                                                                                                   tGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGccc                                 <  7:161496/149‑1 (MQ=255)
                                                                                                                    gccatcgccatcGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGcc                                  >  5:29763/1‑147 (MQ=255)
                                                                                                                       atcgccatcgGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTg                                              <  2:201161/132‑1 (MQ=255)
                                                                                                                       atcgccatcgGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTg                                              >  1:201161/1‑132 (MQ=255)
                                                                                                                        tcgccatcgGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGgcg                            >  3:37525/1‑149 (MQ=255)
                                                                                                                            catcgGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGgcgccgc                        >  3:220862/1‑149 (MQ=255)
                                                                                                                                 gCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCTCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCgg                   <  8:15342/149‑1 (MQ=255)
                                                                                                                                 gCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGACCTGGGCTTCGACGCCCAGGCGCCGCGCCgg                   >  4:273308/1‑149 (MQ=255)
                                                                                                                                 gCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCgg                   >  4:77278/1‑149 (MQ=255)
                                                                                                                                   cTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGAGTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGa                 <  3:208373/149‑1 (MQ=255)
                                                                                                                                      gCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCt                                          <  7:163666/121‑1 (MQ=255)
                                                                                                                                      gCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCt                                          >  8:163666/1‑121 (MQ=255)
                                                                                                                                       cc‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAg             >  5:336962/1‑149 (MQ=255)
                                                                                                                                        c‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGAATACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAgg            >  6:311623/1‑149 (MQ=255)
                                                                                                                                              cgcTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAGGTgggg       <  7:238061/149‑1 (MQ=255)
                                                                                                                                               gcTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAGGTGGGGc      <  6:336962/149‑1 (MQ=255)
                                                                                                                                                   gCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAGGTGGGGCAAc   <  3:77278/148‑1 (MQ=255)
                                                                                                                                                   gCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAGGTGGGGCAACt  >  2:21079/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCCGCGAGCAGGGGCTGAAGGCGCAGTTGCAAGCCAGCGCCGAGCGGGTTACCGGGCGTGTCGACCAAGCCTATCTTGGGCAGGAGCAGGCCCTGCAAGGCGAGCTGCAGCGCAATGCCATCGCCATCGGCCTGGCC‑ACGGCGCTGGCGTTGCTTGTCGGCGTGTTGGCGGCCTGGCTGATTACCCGCGCTGTGGTCGGCCCGTTGAAGCATGTGATCGCCCGCGCCCAGCGCATTGCGGCGGGTGAACTGGGCTTCGACGCCCAGGCGCCGCGCCGGGATGAGGTGGGGCAACT  >  NC_002947/2045550‑2045844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑