Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,063,192	C→A	9.0%	intergenic (+49/‑158)	PP_1838 → / → PP_1839	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,063,192	0	C	A	9.0%	142.9 / 6.7	56	intergenic (+49/‑158)	PP_1838/PP_1839	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base A (2/3);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

CGGAAATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAG  >  NC_002947/2063049‑2063337                                                                                                                                                  |                                                                                                                                                  cGGAAATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg                                                                                                                                               >  1:161994/1‑148 (MQ=255)    aaaTGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGcaca                                                                                                                                           <  8:48107/149‑1 (MQ=255)       tGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAggg                                                                                                                                        <  4:244599/149‑1 (MQ=255)        gCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg                                                                                                                                               <  1:336817/141‑1 (MQ=255)        gCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg                                                                                                                                               >  2:336817/1‑141 (MQ=255)         cTCAAGAAGCTGGAAGAGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAgtgt                                                                                                                                       <  4:35633/148‑1 (MQ=255)          tCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAAGGtcc                                                                                                                                     >  2:76163/1‑147 (MQ=255)            aagaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg                                                                                                                                               <  5:321181/137‑1 (MQ=255)            aagaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGAACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg                                                                                                                                               >  6:321181/1‑137 (MQ=255)              gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGcaca                                                                                                                                           <  1:37861/139‑1 (MQ=255)              gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGcaca                                                                                                                                           >  2:37861/1‑139 (MQ=255)                 gCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                              <  2:161994/149‑1 (MQ=255)                 gCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGACCTGCACAAGGTCCGAAcc                                                                                                                                >  3:259464/1‑147 (MQ=38)                   tGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTtgt                                                                                                                            >  7:190096/1‑149 (MQ=255)                    ggAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTtgtg                                                                                                                           >  2:93259/1‑149 (MQ=255)                            atatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGt                                                                                                                                       <  4:60462/129‑1 (MQ=255)                            atatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGt                                                                                                                                       >  3:60462/1‑129 (MQ=255)                             tatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCaag                                                                                                                       >  3:93579/1‑142 (MQ=255)                             tatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGGGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCaag                                                                                                                       <  4:93579/144‑3 (MQ=255)                              atCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgt                                                                                                                 <  8:36224/149‑1 (MQ=255)                               tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgtg                                                                                                                <  8:190096/149‑1 (MQ=255)                               tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgtg                                                                                                                <  4:242233/149‑1 (MQ=255)                               tCCAGACCGCGACCAAGAGCCTGCTCGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTATGACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCCCGCCCACAGGCATTGCCCAGGGTTAGGACCTTGTGCAGGTCCagt                                                                                                                 >  1:298058/1‑149 (MQ=255)                                ccAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGGCCTGTGc                                                                                                               >  5:127111/1‑149 (MQ=255)                                 cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCtgtgg                                                                                                               <  4:259464/148‑2 (MQ=21)                                    aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCCCGGGCTTTCCCGCTCCCACAGGCATTGCACCGGGTTCGGACCATGTGCAGGTCCTGTgcgcgc                                                                                                           >  4:236611/1‑149 (MQ=255)                                    aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCAT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGc                                                                                                           <  1:69321/149‑1 (MQ=18)                                     ccGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCg                                                                                                          >  3:45540/1‑149 (MQ=255)                                      cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCgg                                                                                                         <  6:156739/149‑1 (MQ=18)                                            cAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGc                                                                                                   >  3:248965/1‑149 (MQ=255)                                              agagCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCGCCCCCAGGCCTTGCACAGGGTTCGGGCCTTGTGCAGGTGCTGTGCGCGCGGGCCAGccc                                                                                                 >  3:146470/1‑149 (MQ=255)                                                    tgctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTTCCCGCGCCCCCAGGCATTGCCCAGGGGTAGGACCATGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt                                                                                           >  3:67382/1‑149 (MQ=255)                                                      ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACTGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt                                                                                           <  5:88268/147‑1 (MQ=255)                                                            aaCACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGGCATTGCACAGGGTTAGGACCTTGTGCAGGTACTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCg                                                                                   >  8:176725/1‑149 (MQ=255)                                                                       cGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTcc                                                                        <  1:93259/149‑1 (MQ=255)                                                                        gtggtgCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGTTAGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc                                                                       >  8:100691/1‑149 (MQ=255)                                                                        gtggtgCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc                                                                       <  4:45540/149‑1 (MQ=255)                                                                           gtgCACGCCACACCGAAGTA‑AACCGGGGCCGCGT‑GGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGCACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                    <  5:303888/149‑1 (MQ=255)                                                                              cACGGCACACCGAAGTA‑AACCGGGGCCGCGTATGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACATTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                  >  1:33803/1‑149 (MQ=255)                                                                                      acCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                             >  5:81218/1‑145 (MQ=255)                                                                                      acCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                             <  6:81218/145‑1 (MQ=255)                                                                                        cGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATcc                                                       >  3:269080/1‑149 (MQ=255)                                                                                         gAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATccc                                                      <  4:248965/149‑1 (MQ=255)                                                                                                     gggCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGTGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGt                                           <  5:140122/149‑1 (MQ=255)                                                                                                       gCCGCGT‑TGCGGCCCATGCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTGCGGACCTGGTCCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCa                                         <  1:43949/149‑1 (MQ=255)                                                                                                                 cGGCCCATTCGCGGGCTTGCCGGCTCCCACCGGCATGGCACAGGGTTCGGACCTTTTGCTGGGCCTGTGCTCGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCTAGGTCAGTTATTCa                                 <  5:220218/148‑1 (MQ=38)                                                                                                                 cGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGCGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa                                 <  6:127111/148‑1 (MQ=255)                                                                                                                     ccATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCTGGGCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCAt                            <  7:135292/149‑1 (MQ=255)                                                                                                                                cTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTgg                 <  2:33803/149‑1 (MQ=255)                                                                                                                                   gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt              >  3:74106/1‑149 (MQ=255)                                                                                                                                     ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc            >  7:157520/1‑149 (MQ=255)                                                                                                                                       gCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt          >  1:328441/1‑149 (MQ=18)                                                                                                                                            cacaGGCATTGCACAGGGATCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAACCCGGCGAATGGGCCCAGCACAATATTCCCCGACTCAACATTATCCCCGCCCCAACGTCAGTTATACAGGCATCACACACCTGGTGTTCGTAGACt     >  4:103200/1‑149 (MQ=255)                                                                                                                                              caGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt     >  7:326843/1‑147 (MQ=255)                                                                                                                                              caGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga   <  1:139141/149‑1 (MQ=255)                                                                                                                                               aGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCGGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag  <  4:269080/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CGGAAATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAG  >  NC_002947/2063049‑2063337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑