| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,063,194 | T→C | 8.9% | intergenic (+51/‑156) | PP_1838 → / → PP_1839 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,063,194 | 0 | T | C | 8.9% | 160.0 / 4.7 | 56 | intergenic (+51/‑156) | PP_1838/PP_1839 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (25/26); new base C (2/3); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01 | |||||||||||
CGGAAATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGA > NC_002947/2063049‑2063338 | cGGAAATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg > 1:161994/1‑148 (MQ=255) aaaTGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGcaca < 8:48107/149‑1 (MQ=255) tGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAggg < 4:244599/149‑1 (MQ=255) gCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg < 1:336817/141‑1 (MQ=255) gCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg > 2:336817/1‑141 (MQ=255) cTCAAGAAGCTGGAAGAGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAgtgt < 4:35633/148‑1 (MQ=255) tCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAAGGtcc > 2:76163/1‑147 (MQ=255) aagaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg < 5:321181/137‑1 (MQ=255) aagaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGAACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTg > 6:321181/1‑137 (MQ=255) gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGcaca < 1:37861/139‑1 (MQ=255) gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGcaca > 2:37861/1‑139 (MQ=255) gCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCtt < 2:161994/149‑1 (MQ=255) gCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGACCTGCACAAGGTCCGAAcc > 3:259464/1‑147 (MQ=38) tGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTtgt > 7:190096/1‑149 (MQ=255) ggAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTtgtg > 2:93259/1‑149 (MQ=255) atatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGt > 3:60462/1‑129 (MQ=255) atatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGt < 4:60462/129‑1 (MQ=255) tatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCaag > 3:93579/1‑142 (MQ=255) tatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGGGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCaag < 4:93579/144‑3 (MQ=255) atCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgt < 8:36224/149‑1 (MQ=255) tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgtg < 4:242233/149‑1 (MQ=255) tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgtg < 8:190096/149‑1 (MQ=255) tCCAGACCGCGACCAAGAGCCTGCTCGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTATGACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCCCGCCCACAGGCATTGCCCAGGGTTAGGACCTTGTGCAGGTCCagt > 1:298058/1‑149 (MQ=255) ccAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGGCCTGTGc > 5:127111/1‑149 (MQ=255) cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCtgtgg < 4:259464/148‑2 (MQ=21) aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCCCGGGCTTTCCCGCTCCCACAGGCATTGCACCGGGTTCGGACCATGTGCAGGTCCTGTgcgcgc > 4:236611/1‑149 (MQ=255) aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCAT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGc < 1:69321/149‑1 (MQ=18) ccGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCg > 3:45540/1‑149 (MQ=255) cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCgg < 6:156739/149‑1 (MQ=18) cAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGc > 3:248965/1‑149 (MQ=255) agagCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCGCCCCCAGGCCTTGCACAGGGTTCGGGCCTTGTGCAGGTGCTGTGCGCGCGGGCCAGccc > 3:146470/1‑149 (MQ=255) tgctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTTCCCGCGCCCCCAGGCATTGCCCAGGGGTAGGACCATGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt > 3:67382/1‑149 (MQ=255) ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACTGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt < 5:88268/147‑1 (MQ=255) aaCACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGGCATTGCACAGGGTTAGGACCTTGTGCAGGTACTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCg > 8:176725/1‑149 (MQ=255) cGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTcc < 1:93259/149‑1 (MQ=255) gtggtgCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGTTAGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc > 8:100691/1‑149 (MQ=255) gtggtgCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc < 4:45540/149‑1 (MQ=255) gtgCACGCCACACCGAAGTA‑AACCGGGGCCGCGT‑GGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGCACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa < 5:303888/149‑1 (MQ=255) cACGGCACACCGAAGTA‑AACCGGGGCCGCGTATGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACATTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt > 1:33803/1‑149 (MQ=255) acCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa < 6:81218/145‑1 (MQ=255) acCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa > 5:81218/1‑145 (MQ=255) cGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATcc > 3:269080/1‑149 (MQ=255) gAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATccc < 4:248965/149‑1 (MQ=255) gggCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGTGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGt < 5:140122/149‑1 (MQ=255) gCCGCGT‑TGCGGCCCATGCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTGCGGACCTGGTCCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCa < 1:43949/149‑1 (MQ=255) cGGCCCATTCGCGGGCTTGCCGGCTCCCACCGGCATGGCACAGGGTTCGGACCTTTTGCTGGGCCTGTGCTCGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCTAGGTCAGTTATTCa < 5:220218/148‑1 (MQ=38) cGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGCGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa < 6:127111/148‑1 (MQ=255) ccATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCTGGGCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCAt < 7:135292/149‑1 (MQ=255) cTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTgg < 2:33803/149‑1 (MQ=255) gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt > 3:74106/1‑149 (MQ=255) ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc > 7:157520/1‑149 (MQ=255) gCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt > 1:328441/1‑149 (MQ=18) cacaGGCATTGCACAGGGATCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAACCCGGCGAATGGGCCCAGCACAATATTCCCCGACTCAACATTATCCCCGCCCCAACGTCAGTTATACAGGCATCACACACCTGGTGTTCGTAGACt > 4:103200/1‑149 (MQ=255) caGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt > 7:326843/1‑147 (MQ=255) caGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga < 1:139141/149‑1 (MQ=255) aGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCGGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag < 4:269080/149‑1 (MQ=255) gctttGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgaga < 3:236611/145‑1 (MQ=255) | CGGAAATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGA > NC_002947/2063049‑2063338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |