Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,063,221 T→G 12.9% intergenic (+78/‑129) PP_1838 → / → PP_1839 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,063,2210TG12.9% 118.9 / 7.1 52intergenic (+78/‑129)PP_1838/PP_1839membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base G (2/5);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

ATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAGTCAT  >  NC_002947/2063078‑2063369
                                                                                                                                                 |                                                                                                                                                    
atCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgt                                                                                                                                                 <  8:36224/149‑1 (MQ=255)
 tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgtg                                                                                                                                                <  8:190096/149‑1 (MQ=255)
 tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgtg                                                                                                                                                <  4:242233/149‑1 (MQ=255)
 tCCAGACCGCGACCAAGAGCCTGCTCGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTATGACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCCCGCCCACAGGCATTGCCCAGGGTTAGGACCTTGTGCAGGTCCagt                                                                                                                                                 >  1:298058/1‑149 (MQ=255)
  ccAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGGCCTGTGc                                                                                                                                               >  5:127111/1‑149 (MQ=255)
   cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCtgtgg                                                                                                                                               <  4:259464/148‑2 (MQ=21)
      aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCCCGGGCTTTCCCGCTCCCACAGGCATTGCACCGGGTTCGGACCATGTGCAGGTCCTGTgcgcgc                                                                                                                                           >  4:236611/1‑149 (MQ=255)
      aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCAT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGc                                                                                                                                           <  1:69321/149‑1 (MQ=18)
       ccGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCg                                                                                                                                          >  3:45540/1‑149 (MQ=255)
        cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCgg                                                                                                                                         <  6:156739/149‑1 (MQ=18)
              cAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGc                                                                                                                                   >  3:248965/1‑149 (MQ=255)
                agagCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCGCCCCCAGGCCTTGCACAGGGTTCGGGCCTTGTGCAGGTGCTGTGCGCGCGGGCCAGccc                                                                                                                                 >  3:146470/1‑149 (MQ=255)
                      tgctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTTCCCGCGCCCCCAGGCATTGCCCAGGGGTAGGACCATGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt                                                                                                                           >  3:67382/1‑149 (MQ=255)
                        ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACTGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt                                                                                                                           <  5:88268/147‑1 (MQ=255)
                              aaCACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGGCATTGCACAGGGTTAGGACCTTGTGCAGGTACTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCg                                                                                                                   >  8:176725/1‑149 (MQ=255)
                                         cGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTcc                                                                                                        <  1:93259/149‑1 (MQ=255)
                                          gtggtgCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGTTAGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc                                                                                                       >  8:100691/1‑149 (MQ=255)
                                          gtggtgCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc                                                                                                       <  4:45540/149‑1 (MQ=255)
                                             gtgCACGCCACACCGAAGTA‑AACCGGGGCCGCGT‑GGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGCACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                                                    <  5:303888/149‑1 (MQ=255)
                                                cACGGCACACCGAAGTA‑AACCGGGGCCGCGTATGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACATTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                                                  >  1:33803/1‑149 (MQ=255)
                                                        acCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                                                             >  5:81218/1‑145 (MQ=255)
                                                        acCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                                                             <  6:81218/145‑1 (MQ=255)
                                                          cGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATcc                                                                                       >  3:269080/1‑149 (MQ=255)
                                                           gAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATccc                                                                                      <  4:248965/149‑1 (MQ=255)
                                                                       gggCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGTGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGt                                                                           <  5:140122/149‑1 (MQ=255)
                                                                         gCCGCGT‑TGCGGCCCATGCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTGCGGACCTGGTCCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCa                                                                         <  1:43949/149‑1 (MQ=255)
                                                                                   cGGCCCATTCGCGGGCTTGCCGGCTCCCACCGGCATGGCACAGGGTTCGGACCTTTTGCTGGGCCTGTGCTCGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCTAGGTCAGTTATTCa                                                                 <  5:220218/148‑1 (MQ=38)
                                                                                   cGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGCGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                                 <  6:127111/148‑1 (MQ=255)
                                                                                       ccATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCTGGGCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCAt                                                            <  7:135292/149‑1 (MQ=255)
                                                                                                  cTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTgg                                                 <  2:33803/149‑1 (MQ=255)
                                                                                                     gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt                                              >  3:74106/1‑149 (MQ=255)
                                                                                                       ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc                                            >  7:157520/1‑149 (MQ=255)
                                                                                                         gCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                                          >  1:328441/1‑149 (MQ=18)
                                                                                                              cacaGGCATTGCACAGGGATCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAACCCGGCGAATGGGCCCAGCACAATATTCCCCGACTCAACATTATCCCCGCCCCAACGTCAGTTATACAGGCATCACACACCTGGTGTTCGTAGACt                                     >  4:103200/1‑149 (MQ=255)
                                                                                                                caGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt                                     >  7:326843/1‑147 (MQ=255)
                                                                                                                caGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga                                   <  1:139141/149‑1 (MQ=255)
                                                                                                                 aGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCGGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag                                  <  4:269080/149‑1 (MQ=255)
                                                                                                                   gctttGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgaga                                 <  3:236611/145‑1 (MQ=255)
                                                                                                                           caGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACTCCTGGTGTTCGTTGTCTGAGAGGGTCCGt                         >  7:334959/1‑148 (MQ=255)
                                                                                                                            aGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt                        >  2:25067/1‑148 (MQ=255)
                                                                                                                              ggTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCaa                     >  6:156525/1‑149 (MQ=255)
                                                                                                                               gTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCaa                     <  3:103200/148‑1 (MQ=255)
                                                                                                                               gTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt                    <  4:74106/149‑1 (MQ=255)
                                                                                                                               gTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt                    <  7:176725/149‑1 (MQ=255)
                                                                                                                                ttCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCACGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt                    >  3:344473/1‑148 (MQ=255)
                                                                                                                                   ggACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGaaa                <  8:326843/149‑1 (MQ=255)
                                                                                                                                      ccTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAagag             >  4:76239/1‑149 (MQ=255)
                                                                                                                                             cAGGTCCTGTGCGAGCGGGCACGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGt            <  8:174802/143‑1 (MQ=255)
                                                                                                                                             cAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGaa       <  5:156525/148‑1 (MQ=255)
                                                                                                                                             cAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAg      <  7:100691/149‑1 (MQ=255)
                                                                                                                                             cAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTAGTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGt            >  7:174802/1‑143 (MQ=255)
                                                                                                                                                gTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAGTCa   >  2:274829/1‑149 (MQ=255)
                                                                                                                                                 tCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAGTCAt  >  8:11085/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTA‑AACCGGGGCCGCGT‑TGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAGTCAT  >  NC_002947/2063078‑2063369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑