Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,107,083 A→T 7.3% C12S (TGT→AGT)  PP_1880 ← outer membrane autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,107,0830AT 7.3% 157.2 / 2.5 55C12S (TGT→AGT) PP_1880outer membrane autotransporter
Reads supporting (aligned to +/- strand):  ref base A (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

TGTCAGAACGGCCGGGCCGATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGCAAAAAATGTAA  >  NC_002947/2106937‑2107224
                                                                                                                                                   |                                                                                                                                             
tGTCAGAACGGCCGGGCCGATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGt                                                                                                                                             >  8:18686/1‑149 (MQ=255)
  tCAGAACGGCCGGGCCGATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTgg                                                                                                                                           >  3:363400/1‑149 (MQ=255)
  tCAGAACGGCCGGGCCGATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTgg                                                                                                                                           >  7:293765/1‑149 (MQ=255)
    aGAACGGCCGGGCCGATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGc                                                                                                                                          >  3:140913/1‑148 (MQ=255)
           ccgggccgATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCGGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGGAGTGGCG‑AAAATCAACGGGCCCGCCAGCACGAGATGAGCGGGTGATGAACACGGGCGAAGGGcc                                                                                                                                  >  4:79330/1‑148 (MQ=255)
            cgggccgATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGt                                                                                                                                 <  8:3958/149‑1 (MQ=255)
             gggccgATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTAAGAGGGTTTCTGCACAGCAGGCACTGGCG‑AAAACCAACGGGCCCGACAGCACGCGATGAGAGGGTGTGCGACAGTGGCGCAGGGCGTg                                                                                                                                >  4:300908/1‑149 (MQ=255)
                ccgATCACACGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGaaa                                                                                                                             <  6:146597/149‑1 (MQ=255)
                  gATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGaaa                                                                                                                             >  7:271537/1‑147 (MQ=255)
                   aTCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGATGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGACCGCCAGCACGATATGAGCGGGTGTTGAAAAGTGGCGAAGGGCGTGAAAAAg                                                                                                                          >  5:124268/1‑149 (MQ=255)
                       cccGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAggcgg                                                                                                                      >  5:210753/1‑149 (MQ=255)
                         cGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCt                                                                                                                    >  7:277145/1‑149 (MQ=255)
                           gTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCg                                                                                                                  <  2:235026/149‑1 (MQ=255)
                              gCTGTCGAGGGTGCGGCCGGCATCCCGCTGAGTATCGGCATCTACGCGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGc                                                                                                                <  4:267304/148‑1 (MQ=255)
                                  tCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCAtt                                                                                                             <  6:162055/147‑1 (MQ=255)
                                         tgctgcCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAg                                                                                                                          >  6:382781/1‑127 (MQ=255)
                                         tgctgcCGGCATCCAGCTGAGTATCGGCATCCACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAg                                                                                                                          <  5:382781/127‑1 (MQ=255)
                                              ccGGCATACAGCTGAGGATCGGCAGCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCGCGGCATTCAGGGAAAACCTc                                                                                                <  6:124268/148‑1 (MQ=255)
                                               cGGCATCCAGCTGAGTATCGGCATCTACGAGGATTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCg                                                                                               >  1:144695/1‑148 (MQ=255)
                                                   aTCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTg                                                                                          <  7:18686/149‑1 (MQ=255)
                                                     ccAGCTGAGTATCGGCATCTACGAGGGTTTCTGTCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGa                                                                                         >  5:285034/1‑148 (MQ=255)
                                                              tATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCGAAAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAAtttt                                                                                <  4:171132/149‑1 (MQ=255)
                                                                tCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTg                                                                              <  4:22754/148‑1 (MQ=255)
                                                                tCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTg                                                                              >  4:168417/1‑148 (MQ=255)
                                                                  ggCATCTACGAGTGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAAAAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCACTCAGGGAAAACCTc                                                                                                >  6:186102/1‑128 (MQ=255)
                                                                  ggCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGGGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTaa                                                                           <  1:261595/149‑1 (MQ=255)
                                                                  ggCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCACTCAGGGAAAACCTc                                                                                                <  5:186102/128‑1 (MQ=255)
                                                                  ggCATCTACGACGCTTTCTGCCCAGCAGTCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAGGTGAACAAATTTTTGTaa                                                                           <  3:300908/149‑1 (MQ=255)
                                                                   gCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGt                                                                             >  6:136685/1‑146 (MQ=255)
                                                                    cATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGa                                                                         <  6:210753/149‑1 (MQ=255)
                                                                         aCGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAg                                                                                                          >  7:305742/1‑111 (MQ=255)
                                                                         aCGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAg                                                                                                          <  8:305742/111‑1 (MQ=255)
                                                                         aCGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGtt                                                                    >  5:62160/1‑149 (MQ=255)
                                                                                   cTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTc                                                           <  5:136685/148‑1 (MQ=255)
                                                                                      cccATCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAATGGCGGCTCGGCATTCAGGGAAAACCTCGAAGt                                                                                           <  3:321214/113‑1 (MQ=255)
                                                                                      cccAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAGGGCGGCTCGGCATTCAGGGAAAACCGCGAAgg                                                                                           >  4:321214/1‑112 (MQ=255)
                                                                                      cccAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTg                                                       <  3:93853/149‑1 (MQ=255)
                                                                                         agcagGCACTGGCG‑AAAAACAACGGGCACGCCATCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGgag                                                    <  7:179483/149‑1 (MQ=255)
                                                                                             ggCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAAc                                                 >  2:257883/1‑148 (MQ=255)
                                                                                              gCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGga                                                     <  4:255566/143‑1 (MQ=255)
                                                                                              gCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGga                                                     >  3:255566/1‑143 (MQ=255)
                                                                                              gCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTG‑AAAAGGCGGCTCGGCATTCAGGGAAACCCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGG‑GAACAATc                                             >  4:52790/1‑149 (MQ=255)
                                                                                                      g‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATa                                       >  4:152129/1‑149 (MQ=255)
                                                                                                         aaaaCAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATAcc                                     <  6:285034/149‑1 (MQ=255)
                                                                                                             caacGGCCACGCCAGCACTACATGAGCGTGGTTTGAACAGGTGAGAAGGGCGTGAACAAGGCTGCTCGGCATTCAGGGAAAACCTCGAATTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGATAACAATCCGCATACCGCGa                                 <  8:125888/149‑1 (MQ=255)
                                                                                                                 gggCCCGCCAGCACGAGATGAGCGGGTGTTGAACTGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGc                              >  2:132413/1‑148 (MQ=255)
                                                                                                                 gggCCCGCCAGCACGAGATGAGCGGGTGTTGAACTGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGc                              >  2:128384/1‑148 (MQ=255)
                                                                                                                             aCGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAg                 >  8:299144/1‑149 (MQ=255)
                                                                                                                                 gaTGAGCGGGTGTTGAACTGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGc             <  1:128384/149‑1 (MQ=255)
                                                                                                                                 gaTGAGCGGGTGTTGAACTGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGc             <  1:132413/149‑1 (MQ=255)
                                                                                                                                 gaTGAGCGGGTGTTGAACAGTGGCGAAGGGCGTTAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGc             <  3:79330/149‑1 (MQ=255)
                                                                                                                                      gCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGCaaaa         >  7:96607/1‑148 (MQ=255)
                                                                                                                                       cGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGCaaaaaa       <  2:144695/149‑1 (MQ=255)
                                                                                                                                       cGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAAGCTAAGCGCGTTAAGTCGCaaaaaa       <  8:271537/149‑1 (MQ=255)
                                                                                                                                             ttGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGCAAAAAATGTaa  <  7:299144/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
TGTCAGAACGGCCGGGCCGATCACCCGGTAGCTGTCGAGTGTGCTGCCGGCATCCAGCTGAGTATCGGCATCTACGAGGGTTTCTGCCCAGCAGGCACTGGCG‑AAAAACAACGGGCCCGCCAGCACGAGATGAGCGGGTGTTGAACAGTGGCGAAGGGCGTGAAAAAGGCGGCTCGGCATTCAGGGAAAACCTCGAAGTGAACAAATTTTTGTAAGACCGTTCTGCTTTTCGCTGGAGAACAATCCGCATACCGCGAGGCTAAGCGCGTTAAGTCGCAAAAAATGTAA  >  NC_002947/2106937‑2107224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑