Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,117,893	T→C	6.5%	C523C (TGT→TGC)	PP_1884 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,117,893	0	T	C	6.5%	180.4 / 2.4	61	C523C (TGT→TGC)	PP_1884	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/31);  new base C (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

GCGGCCCCCTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAGTCGGTAGAGGGCTCCTCGGAT  >  NC_002947/2117745‑2118040                                                                                                                                                     |                                                                                                                                                    gcgGCCCCCTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGACGGCGAGCGGCTCATGCGCCGCGCCATTGCCTGt                                                                                                                                                     >  7:276272/1‑149 (MQ=255)       cccTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTggc                                                                                                                                               <  1:366323/149‑1 (MQ=255)        ccTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTggcg                                                                                                                                              <  4:155567/149‑1 (MQ=255)        ccTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTggcg                                                                                                                                              <  4:149857/149‑1 (MQ=255)        ccTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTggcg                                                                                                                                              <  6:139494/149‑1 (MQ=255)         cTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGATATTGCCTGTTTTggcgg                                                                                                                                             >  4:257190/1‑149 (MQ=255)            gCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTggcggc                                                                                                                                            <  4:317335/147‑1 (MQ=255)              cGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGtttt                                                                                                                                                  <  6:335773/139‑1 (MQ=255)              cGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGtttt                                                                                                                                                  >  5:335773/1‑139 (MQ=255)                cagcaAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCt                                                                                                                                      <  4:145753/149‑1 (MQ=255)                cagcaAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCt                                                                                                                                      >  1:48719/1‑149 (MQ=255)                 agcaAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGATATTGCCTGTTTTGGCGGCGTCGCTc                                                                                                                                     <  3:257190/149‑1 (MQ=255)                 agcaAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTc                                                                                                                                     <  6:111021/149‑1 (MQ=255)                  gcaAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTc                                                                                                                                     >  7:148520/1‑148 (MQ=255)                    aaGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCca                                                                                                                                   >  5:129285/1‑148 (MQ=255)                     agagCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCcaca                                                                                                                                 <  6:337385/149‑1 (MQ=255)                      gagCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCTCTCCACAg                                                                                                                                <  5:247034/149‑1 (MQ=255)                         ccGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCt                                                                                                                              >  1:36039/1‑148 (MQ=255)                         ccGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGAGGCGTCGCTCCACAGCt                                                                                                                              >  3:241607/1‑148 (MQ=255)                           gCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGAc                                                                                                                           >  4:318535/1‑149 (MQ=255)                                   cAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGAGGCGTCGCTCCACAGCTGACCACCTTCg                                                                                                                   >  8:72073/1‑149 (MQ=255)                                            tCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGAAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCAccc                                                                                                          >  1:336303/1‑149 (MQ=255)                                               tttAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCAcccccc                                                                                                       >  8:36816/1‑149 (MQ=255)                                               tttAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCAcccccc                                                                                                       <  5:236198/149‑1 (MQ=255)                                                        gTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGaaaaaa                                                                                              >  1:345765/1‑149 (MQ=255)                                                         tACATGGACGAATTGCTCGATAAGACCCTCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAAt                                                                                             <  1:226062/149‑1 (MQ=255)                                                           cATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCg                                                                                           >  5:172326/1‑149 (MQ=255)                                                            ttGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGt                                                                                          <  7:87688/148‑1 (MQ=255)                                                             tGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTg                                                                                         <  2:48719/149‑1 (MQ=255)                                                              ggaggaATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGa                                                                                        >  2:7448/1‑149 (MQ=255)                                                              ggaggaATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGa                                                                                        >  6:144084/1‑149 (MQ=255)                                                               gaggaATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGa                                                                                        <  4:90436/148‑1 (MQ=255)                                                               gaggaATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGGCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGa                                                                                        <  8:67789/148‑1 (MQ=255)                                                                  gaATTGATCGGTAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCa                                                                                    >  6:345374/1‑149 (MQ=255)                                                                         tCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGgcagc                                                                             <  6:12409/149‑1 (MQ=255)                                                                           gATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCAc                                                                           >  7:182297/1‑149 (MQ=255)                                                                               aGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTg                                                                       <  8:148520/149‑1 (MQ=255)                                                                                 aCCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGCCATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTg                                                                       >  2:214941/1‑147 (MQ=255)                                                                                 aCCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCa                                                                     <  6:129285/149‑1 (MQ=255)                                                                                  ccATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAg                                                                    <  1:7448/149‑1 (MQ=255)                                                                                           tGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCAcc                                                           >  6:28887/1‑149 (MQ=255)                                                                                                tttGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTccc                                                      >  4:299914/1‑149 (MQ=255)                                                                                                tttGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTccc                                                      >  1:227384/1‑149 (MQ=255)                                                                                                  tggtggCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCg                                                     <  1:375449/148‑1 (MQ=255)                                                                                                       gcgcGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCa                                                <  2:36039/148‑1 (MQ=255)                                                                                                         gcgATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCa                                                             <  6:203770/133‑1 (MQ=255)                                                                                                         gcgATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCa                                                             >  5:203770/1‑133 (MQ=255)                                                                                                         gcgATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGgc                                                   <  6:224675/143‑1 (MQ=255)                                                                                                         gcgATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGGTGCAGCTTGCCACCGTCCCGgc                                                   >  5:224675/1‑143 (MQ=255)                                                                                                             tACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAAc                                         <  6:172326/149‑1 (MQ=255)                                                                                                                    aGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCt                                  <  8:182297/149‑1 (MQ=255)                                                                                                                        gAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTc                              >  1:178352/1‑149 (MQ=255)                                                                                                                         aGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCg                             <  5:345374/149‑1 (MQ=255)                                                                                                                          gCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCgg                            <  7:36816/149‑1 (MQ=255)                                                                                                                             gCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCAACAGCTGACCACGTTCGCCGTCACACCCCTGGAAAAAATCGTGACCCCCGGCAGCAAGTTGCAGCTTGCCACCGTCCCGGCGCACGCCAACGTACCCTGGACGGcc                          >  6:97508/1‑148 (MQ=255)                                                                                                                               acaTGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAg                       <  5:144084/149‑1 (MQ=255)                                                                                                                                 aTGCCCCGCGACATTGCCTGCTTTGGCGGCGTCGCTCCACAGCTGCCCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGc                           <  7:124655/143‑1 (MQ=255)                                                                                                                                 aTGCCCCGCGACATTGCCTGCTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGc                           <  7:129828/143‑1 (MQ=255)                                                                                                                                 aTGCCCCGCGACATTGCCTGCTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGc                           >  8:129828/1‑143 (MQ=255)                                                                                                                                 aTGCCCCGCGACATTGCCTGCTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGACACCGTCCCGGCGCAAGCCACCGTACCCTGGTCgga                           >  8:124655/1‑142 (MQ=255)                                                                                                                                      ccGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAGTCGGTag                <  2:227384/149‑1 (MQ=255)                                                                                                                                      ccGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTGCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAGTCGGTag                <  6:349624/149‑1 (MQ=255)                                                                                                                                       cgcgACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAGTCGGTa                 >  7:10715/1‑147 (MQ=255)                                                                                                                                                    gTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAGTCGGTAGAGGGCTCCTCGGAt  >  8:7496/1‑149 (MQ=255)                                                                                                                                                     ttttGGCGGCGTCGCTCCACAGCTGCCCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAGTCGGTAGAGGGCTCCTCGGa   <  6:283125/147‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCGGCCCCCTTGCCGCAGCAAGAGCCGCACTCGCCAAGGACCTTCGTTTAGACCTGTACATGGAGGAATTGATCGATAAGACCATCAAACTGAACTTTGGTGGCGCGATACTCGCAGGCGAGCGGCACATGCCCCGCGACATTGCCTGTTTTGGCGGCGTCGCTCCACAGCTGACCACGTTCGCCGTCACCCCCCTGGAAAAAATCGTGACCCACGGCAGCACGTTGCAGCTTGCCACCGTCCCGGCGCAAGCCAACGTAACCTGGTCGGCCGAGTCGGTAGAGGGCTCCTCGGAT  >  NC_002947/2117745‑2118040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑