Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,138,409 T→G 9.2% L171R (CTG→CGG)  yadG → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,138,4090TG 9.2% 181.3 / 7.9 63L171R (CTG→CGG) yadGABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base G (2/4);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAG  >  NC_002947/2138263‑2138553
                                                                                                                                                  |                                                                                                                                                
tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTgc                                                                                                                                                >  8:128184/1‑149 (MQ=255)
    cTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGcgccg                                                                                                                                            >  8:290506/1‑149 (MQ=255)
       ggCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCt                                                                                                                                          <  1:166477/148‑1 (MQ=255)
        gCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCg                                                                                                                                        <  7:119315/149‑1 (MQ=255)
         ccTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGa                                                                                                                                       <  3:244084/149‑1 (MQ=255)
          cTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                      <  8:7824/149‑1 (MQ=255)
          cTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGAGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                      <  7:244050/149‑1 (MQ=255)
               ggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGa                                                                                                                                 <  3:56797/149‑1 (MQ=255)
               ggACAAACGTGATGGGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCAGTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGa                                                                                                                                 <  5:70478/149‑1 (MQ=255)
                gACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAg                                                                                                                                <  6:143258/149‑1 (MQ=255)
                           tgtgCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTg                                                                                                                     <  6:346684/149‑1 (MQ=255)
                                aGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCt                                                                                                                 >  2:285286/1‑148 (MQ=255)
                                aGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCt                                                                                                                 >  3:213999/1‑148 (MQ=255)
                                   cgcgTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAAc                                                                                                             <  3:189217/149‑1 (MQ=255)
                                          gttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAgg                                                                                                      >  8:57411/1‑149 (MQ=255)
                                             gTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGc                                                                                                    <  4:213999/148‑1 (MQ=255)
                                              tCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCAt                                                                                                  <  5:187353/149‑1 (MQ=255)
                                               ccGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCAt                                                                                                  >  1:176490/1‑148 (MQ=255)
                                                 ggcggcATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCAc                                                                                               >  5:39580/1‑149 (MQ=255)
                                                    ggcATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcat                                                                                            >  1:3055/1‑149 (MQ=255)
                                                       aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcatcat                                                                                         >  1:192370/1‑149 (MQ=255)
                                                       aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcatcat                                                                                         >  7:18320/1‑149 (MQ=255)
                                                       aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCAACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAACGGCATCACcatcat                                                                                         >  5:242379/1‑149 (MQ=255)
                                                        tGAAGCGGCGCCTGATGTTTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCatcatc                                                                                        <  7:57411/149‑1 (MQ=255)
                                                        tGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCatcatc                                                                                        <  7:290506/149‑1 (MQ=255)
                                                        tGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCatcatc                                                                                        <  6:39580/149‑1 (MQ=255)
                                                         gAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                      <  6:323818/102‑1 (MQ=255)
                                                         gAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                      >  5:323818/1‑102 (MQ=255)
                                                                cgcCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTcaccac                                                                                <  2:192370/149‑1 (MQ=255)
                                                                     gatgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCAccacccac                                                                            >  7:193234/1‑148 (MQ=255)
                                                                      atgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTa                                                                          >  7:179018/1‑149 (MQ=255)
                                                                        gatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCCCCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCAATGAGCTGAACCAGAAGGGCATCACCATCATCATAACCCACCACtctc                                                                        >  4:246584/1‑149 (MQ=255)
                                                                         atTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGGGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCt                                                                       <  5:330107/149‑1 (MQ=255)
                                                                            gCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCAGTGAGCTGAACGAGAAGGGCATCACCATCATCCTCACCACCCACTATCTgg                                                                     <  8:179018/148‑1 (MQ=255)
                                                                            gCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAGCCAGACCGGCATCACCATCCTCCTGCCCAGCCACTATCTgg                                                                     >  8:306872/1‑148 (MQ=255)
                                                                                    aTTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCt                                                             >  8:336032/1‑148 (MQ=255)
                                                                                         tCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGGATAGCCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagcag                                                       >  1:313003/1‑149 (MQ=255)
                                                                                         tCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagcag                                                       <  7:336032/149‑1 (MQ=255)
                                                                                            aCGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtg                                                    >  7:79983/1‑149 (MQ=255)
                                                                                             cGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGTGCTGCGCCGCTCGCTGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTACCCACCCACGATCTGGAAGAGGCTGAGCAGCtgt                                                   >  1:92805/1‑149 (MQ=255)
                                                                                             cGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtgt                                                   <  1:146538/149‑1 (MQ=255)
                                                                                                aCCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGcc                                                <  7:66975/149‑1 (MQ=255)
                                                                                                aCCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGcc                                                >  1:299859/1‑149 (MQ=255)
                                                                                                aCCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGcc                                                >  2:103152/1‑149 (MQ=255)
                                                                                                 ccGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCg                                               <  2:176490/149‑1 (MQ=255)
                                                                                                               ccTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGcatca                                 >  6:256818/1‑149 (MQ=255)
                                                                                                                 tGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCatcatc                               >  4:8008/1‑149 (MQ=255)
                                                                                                                 tGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCatcatc                               >  8:4888/1‑149 (MQ=255)
                                                                                                                       tatccACCCCGGCGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCAt                         >  6:179148/4‑149 (MQ=255)
                                                                                                                         cccACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCg                              >  1:256433/1‑142 (MQ=255)
                                                                                                                         cccACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCg                              <  2:256433/142‑1 (MQ=255)
                                                                                                                         cccACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATgg                       <  6:195368/149‑1 (MQ=255)
                                                                                                                          ccACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGag                                                          >  1:245744/1‑113 (MQ=255)
                                                                                                                          ccACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGag                                                          <  2:245744/113‑1 (MQ=255)
                                                                                                                                ccgGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCAtc                <  3:202225/149‑1 (MQ=255)
                                                                                                                                     gTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCga           <  7:306872/149‑1 (MQ=255)
                                                                                                                                      tccacatCGAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCAtcgtc             >  4:200487/6‑146 (MQ=255)
                                                                                                                                          tatCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCga           >  8:320558/1‑144 (MQ=255)
                                                                                                                                          tatCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCga           <  7:320558/144‑1 (MQ=255)
                                                                                                                                              gAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCa   <  7:4888/148‑1 (MQ=255)
                                                                                                                                              gAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAg  <  2:299859/149‑1 (MQ=255)
                                                                                                                                              gAGCTGCGCCGCGCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCa   <  7:128184/148‑1 (MQ=255)
                                                                                                                                              gAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCa   <  3:200487/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAG  >  NC_002947/2138263‑2138553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑