| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,166,708 | T→A | 10.7% | intergenic (‑552/‑229) | PP_5483 ← / → PP_1921 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,166,708 | 0 | T | A | 10.7% | 116.2 / 6.2 | 47 | intergenic (‑552/‑229) | PP_5483/PP_1921 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/21); new base A (3/2); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
CCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGC > NC_002947/2166561‑2166853 | ccTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCtt > 3:332243/1‑149 (MQ=255) gATCAGTAAGTTAGTCTCAGCAACCTATTGCTGAGGGTTTAAGGATCGCCGTTGGCCTTTATGTGAAACGACCCGCTCGTGGAGGGGGATGATCCGAGGGCTGTTGGGGTTTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAg < 2:130734/149‑1 (MQ=255) gATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAg < 6:25771/149‑1 (MQ=255) gATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAg > 7:195038/1‑149 (MQ=255) tCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGt > 3:87727/1‑149 (MQ=255) gAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTg > 7:350755/1‑148 (MQ=255) ctatgtctctCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCa < 7:301382/142‑1 (MQ=255) gTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgattga < 5:243199/149‑6 (MQ=255) ctctCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgta < 1:362905/149‑1 (MQ=255) tctcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCa > 8:301382/1‑141 (MQ=255) tctcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtag > 2:126205/1‑149 (MQ=255) ttGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagta < 8:195038/149‑1 (MQ=255) ttGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGAGGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagta < 7:196702/149‑1 (MQ=255) cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg < 7:30472/148‑1 (MQ=255) cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg > 8:30472/1‑148 (MQ=255) agagGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtaggga < 4:87727/149‑1 (MQ=255) agagGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtaggga < 8:350755/149‑1 (MQ=255) gggTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATTCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggag > 2:330392/1‑147 (MQ=255) ggTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtag < 4:332243/149‑1 (MQ=255) tAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGtagggagtagggagtagggagtagggag > 2:8273/1‑149 (MQ=255) gATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGTGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGgagtagggagtagggagtagggagtagg < 1:126205/149‑1 (MQ=255) aaCGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGc > 1:249579/1‑88 (MQ=255) aaCGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGc < 2:249579/88‑1 (MQ=255) cgcccgtcTCCGGGTGCGGGATGATCCTAGGGCTGTTGGGGTGTCGCTGTTTGATTGAAGCTCAATCAAGCTGGCTTCCAGGTTGCAGGTCGTAGTTATTAGGTAGTAGGGAGTTGGGATTAGGGGCAGGGAGCGTCGGCGCTGGTGCa < 8:151530/142‑1 (MQ=255) tcccGCTCCAGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCACGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTg > 2:102358/2‑145 (MQ=255) ccGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCGTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGCtt > 4:350068/1‑149 (MQ=255) gCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgt > 7:133678/1‑87 (MQ=255) gCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgt < 8:133678/87‑1 (MQ=255) cTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGt < 5:171909/147‑1 (MQ=255) gCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCg > 3:89689/1‑148 (MQ=255) ggATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAg > 3:262364/1‑148 (MQ=255) ggATGATCCGAGGGCTGTTCGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGCTTCTGTCGAGCGAAg > 2:375267/1‑148 (MQ=255) gatgatCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAg > 5:114848/1‑147 (MQ=255) tCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGgaga < 1:8273/149‑1 (MQ=255) tCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGga > 5:300836/1‑147 (MQ=255) gAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGagagag < 6:114848/149‑1 (MQ=255) gCTGTTGGGGTGTGGGTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGCGTAGGGGCAGGGAGAGGCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGaggagg < 3:350068/149‑1 (MQ=255) ttGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGGCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGtt < 7:351424/148‑1 (MQ=255) caggTTGATTGAGCTTCACTCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTg > 4:109707/3‑149 (MQ=255) gTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGct < 1:330392/148‑1 (MQ=255) aaGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCGGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGGTactatgaa > 2:296582/1‑147 (MQ=255) ctgcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTactatgactat > 7:191577/4‑145 (MQ=255) ctgcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTactatgactat < 8:191577/142‑1 (MQ=255) tgcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCa > 2:3497/3‑149 (MQ=255) tcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGATAGGAGGGTTGGTGTTATTTTGCTACTATGACGATGCGCac > 7:38746/1‑149 (MQ=255) gcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCac > 3:46146/2‑149 (MQ=255) cTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCACGAGATACGAGGGTTGGTGTTATTTTGCTaatatga > 3:346596/1‑134 (MQ=255) gCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGc < 4:89689/148‑1 (MQ=255) | CCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGC > NC_002947/2166561‑2166853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |